Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/10315
標題: Vancomycin/磷酸鈣鹽複合塗層於已披覆生醫陶瓷Ti6Al4V植入材之研究
The Study of Vancomycin/CaP Composite Coating on Post Bioceramics Coated Ti6Al4V
作者: Chen, Yi-An
陳逸安
關鍵字: vancomycin
萬古黴素
CaP
titanium
implant
磷酸鈣鹽
植入材
鈦合金
出版社: 材料科學與工程學系所
引用: 1. E. Cevher, Z. Orhan, L. Mülazimoğlu, D. Şensoy, M. Alper, A. Yildiz, and Y. Özsoy. Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres. Int J Pharm 2006;24:127-135. 2. Waldvogel FA, Medoff G, and Swartz MN. Osteomyelitis: a review of clinical features, therapeutic considerations and unusual aspects. N Engl J Med 1970;282(6):316-322. 3. M. Lucke, G. Schmidmaier, S. Sadoni, B. Wildemann, R. Schiller, N.P. Haas, and M. Raschke. Gentamycin coating of metallic implants reduces implant-related osteomyelitis in rats. Bone 2003;32:521-531. 4. A. Sudo, M. Hasegawa, A. Fukuda, and A. Uchida. Treatment of infected hip arthroplasty with antibiotic-impregnated calcium hydroxyapatite. J Arthro 2008;23(1):145-150. 5. V. Alt, A. Bitschnau, J. Österling, A. Sewing, C. Meyer, R. Kraus, S. A. Meissner, S. Wenisch, E. Domann, and R. Schnettler. The effects of combined gentamicin-hydroxyapatite coating for cementless joint prostheses on the reduction of infection rates in a rabbit infection prophylaxis. Biomaterials 2006;27:4627-4634. 6. M. Stigter, J. Bezemer, K. de Groot, and P. Layrolle. Incorporation of different antibiotics into carbonated hydroxyapatite coatings on titanium implants, release and antibiotic efficacy. J Controlled Release 2004;99:127-137. 7. R. W. King. Osteomyelitis. eMedicine from WebMD 2006. 8. M. S. Rouse, J. M. Steckelberg, and R. Patel. In vitro activity of ceftobiprole, daptomycin, and vancomycin against methicillin-resistant Staphylococci associated with endocarditis and bone and joint infection. Diag Micro Infec Dis 2007;58:363-365. 9. S. Radin and P. Ducheyne. Controlled release of vancomycin from thin sol-gel films on titanium alloy fracture plate material. Biomaterials 2007;28:1721-1729. 10. S. Radin, J. T. Campbell, P. Ducheyne, and J. M. Cuckler. Calcium phosphate ceramic coating as carriers of vancomycin. Biomaterials 1997;18:777-782. 11. F. Chai, J. C. Hornez, N. Blanchemain, C. Neut, M. Descamps, and H. F. Hildebrand. Antibacterial activation of hydroxyapatite (HA) with controlled porosity by different antibiotics. Biomolecular Engineering 2007;24:510-514. 12. B. S. Ng, I. Annergren, A. M. Soutar, K. A. Khor, and A. E. W. Jarfors. Characterisation of a duplex TiO2/CaP coating on Ti6Al4V for hard tissue replacement. Biomaterials 2005;26:1087-1095. 13. C. M. Lin, and S. K. Yen. Characterization and bond strength of electrolytic HA/TiO2 double layers for orthopaedic applications. J Mater Sci-Mater Med 2005;16:889-897. 14. Y. Li, I. S. Lee, F. Z. Cui, and S. H. Choi. The biocompatibility of nanostructured calcium phosphate coated on micro-arc oxidized titanium. Biomaterials 2008;29:2025-2032. 15. S. K. Yen, and C. M. Lin. Cathodic reactions of electrolytic hydroxyapatite coating on pure titanium. Mater Chem Phys 2002;77:70-76. 16. S. K. Yen, and C. M. Lin. Characterization of electrolytic Al2O3/CaP composite coating on pure titanium. J Electrochem Soc 2002;149(5):D79-D87. 17. C. M. Lin, and S. K. Yen. Charaterization of electrolytic TiO2 coating on Ti for biomedical applications. J Electrochem Soc 2004;151(12):D127-D133. 18. K. L. Kirkwood, R. Dziak, and P. G. Bradford. Hormonal regulation of inositol trisphosphate recepter gene expression in human osteoblastic-like cells and rat primary osteoblastic cell cultures. J Bone Mineral Res 1996;11:1889-1896. 19. R. A. Ramires, A. Romito, F. Cosentino, and E. Milella. The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour. Biomaterials 2001;22:1467-1474. 20. O. H. Lowry, N. R. Roberts, M. L. Wu, W. S. Hixon, and E. J. Crawford. The quantitative histochemistry of brain II. J Biolog Chem 1953:19-37. 21. G. Holzer, T. A. Einhorn, and R. J. Majeska. Estrogen regulation of growth and alkaline phosphatase expression by cultured human bone marrow stromal cells. J Orthopaedic Res 2002;20(2):281-288. 22. A. Dion, M. Langman, G. Hall, M. Filiaggi. Vancomycin release behaviour from amorphous calcium polyphosphate matrices intended for osteomyelitis treatment. Biomaterials 2005;26:7276-7285. 23. G. S. Stein, J. B. Lian, J. L. Stein, A. J. Van Wijnen and M. Montecino. Transcriptional control of osteoblast growth and differentiation. Physiological Reviews 1996;76:593-629. 24. J. Billiard, R. A. Moran, M. Z. Whitley, M. Chatterjee-Kishore, K. Gillis, E. L. Brown, B. S. Komm, and P. V. N. Bodine. Transcriptional profiling of human osteoblast differentiation. J Cellular Biochem 2003;89:389-400.
摘要: To minimize the probability of bacteria caused infections and to enhance osteointergration between bone and implants, vancomycin-CaP composite coating on post bioceramics coated titanium was carried out in this study. The vancomycin-CaP composite coated specimens were characterized by XRD for the crystal structures, FESEM/EDS analysis for the morphology and the composition, FTIR for the qualitative analysis, UV/Vis analysis for the loading and releasing of vancomycin, and cell culture test for the bioactivity. The cell proliferation, differentiation, and mineralization were evaluated by methyl thiazole tetrazolium (MTT), alkaline phosphatase (ALP), and osteocalcin assays, respectively. The average content of vancomycin on the composite coating is 46.4 μg/cm2, and the release of vancomycin will reach over 90%, higher than the minimum bactericidal concentration (MBC) but less than the minimum toxicity concentration (MTC), in the in vitro release test with 3 ml PBS solution for 24 hours. The results of cell culture tests also indicated that this coating, containing antibiotic vancomycin, is not harmful to the bioactivity of osteoblast-like cell, such as adhesion, proliferation, differentiation, and mineralization.
本研究主要為了降低手術置入植入材時所伴隨的感染風險,將vancomycin與磷酸鈣鹽(vancomycin-CaP)複合塗層進ㄧ步塗佈於已具生醫陶瓷鍍層之Ti6Al4V合金上,希望植入後能夠抑制細菌感染並且隨著CaP的降解釋放出鈣離子促進細胞鈣化。分別利用X光繞射儀分析晶粒結構、以FTIR定性,再以掃描式電子顯微鏡(SEM/EDS)進行成分和表面型態之分析,針對藥物以UV做含量測定及釋放試驗,最後以細胞培養、MTT、ALP及Osteocalcin測試成骨細胞在試片上貼附、增殖、分化與鈣化之情形。結果顯示vancomycin-CaP之複合塗層,含有vancomycin 26.6 μg/cm2,並且在24小時內於3 ml之磷酸鹽緩衝溶液中釋放超過90%,高於最低殺菌濃度且不超過最低致毒濃度,而攜帶vancomycin之塗層於細胞培養實驗也顯示塗層並不會對骨細胞之貼附、增殖、分化與鈣化造成負面影響。
URI: http://hdl.handle.net/11455/10315
其他識別: U0005-2507200609451000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2507200609451000
Appears in Collections:材料科學與工程學系

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