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標題: 評估市售寧素製劑處理Eimeria tenella卵囊對雞之免疫效能
Evaluation of the immunization effects of Eimeria tenella oocysts treated with commercial neem in chicken
作者: 施國偉
See, Koh-Vi
關鍵字: neem
Eimeria tenella
Eimeria tenella
出版社: 獸醫學系暨研究所
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摘要: 雞之球蟲症是由Eimeria屬原蟲感染造成,是養雞事業中常見的寄生蟲性疾病。球蟲症造成的損失於臨床上可見雞隻體重減輕、飼料換肉率下降及高度的死亡率;於經濟上需花費鉅額的費用在球蟲的治療及預防。 本研究利用未芽胞化的E. tenella卵囊,將其置入0.2%由印度苦楝萃取之市售寧素製劑讓球蟲卵囊芽胞化,當這些E. tenella卵囊充分芽胞化後,將其以不同的劑量接種雞隻。兩週後,再用50,000顆2.5%重鉻酸鉀芽胞化的E. tenella卵囊進行攻毒實驗。結果顯示,0.2%市售寧素製劑不但可以讓球蟲卵囊芽胞化,經放置3個月後仍然具有感染能力。而在對雞隻之保護性免疫效果方面,可見雞隻接種經寧素製劑處理之芽胞化卵囊,其卵囊抑制指數、體重及增重的表現皆比攻毒後的陽性對照組來得好。 綜合上述,接種3,000顆寧素製劑處理組的雞隻,能使其得到較好的免疫保護力。此外,發現0.2%寧素對於雞球蟲卵囊的芽胞化效果良好,因此基於環境的保護,降低重金屬的污染,未來可以使用寧素來取代重鉻酸鉀,作為球蟲卵囊的孵化液。
Coccidiosis, caused by various apicomplexan parasites of the genus Eimeria, is a common disease of commercially reared chickens. The major injures of coccidiosis include the reductive weight gain, poor feed-conversion, high levels of mortality, economic loss which is caused by the production losses and costs for treatment or prevention. In this study, unsporulated oocysts of E. tenella were treated with 0.2% commercial neem for sporulation and the chickens were then infected with different doses of treated sporulated oocysts. Two weeks later, all chickens were challenged with 50,000 wild-type E. tenella oocysts which were sporulated in 2.5% potassium dichromate solution. Results indicated that sporulation occurred in the 0.2% commercial neem and three months later the oocysts still possessed infectious activity. The result of immunoresponsitivity showed that the non-immunized positive group had lower reduction percentage in oocyst output, lighter body weight and lower weight gain compared with immunized groups. In summary, the group of chicken inoculated with neem-treated 3,000 oocysts has shown better recovering activity than other groups. In addition, neem shows high performance to sporulated E. tenella oocysts. The use of neem to sporulate could be a substitute for potassium dichromate in experimental infection model in the future, to reduce the risk of residual heavymetals on environment.
其他識別: U0005-2807201117073000
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