Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13105
標題: 以水針方式經由不同穴位給藥及低劑量PGF2α對於誘導乳牛黃體溶解的效果
Effects of aquapuncture with different acupuncture point and low dosage of PGF2α on inducing leutolysis in dairy cows
作者: 黃偉嘉
Huang, Wei-Chia
關鍵字: luteolysis
黃體溶解
BAI-HUI acupoint
aquapuncture
百會穴
水針
出版社: 獸醫學系暨研究所
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摘要: 生理緊迫而造成泌乳牛內分泌紊亂,導致其鈍性發情(silent heat)甚至不發情的情形,即便以PGF2α誘導黃體溶解,在臨床上也常觀察到黃體溶解作用延遲或是黃體溶解不完全的情形。本研究之目的,一方面是以高張溶液(50%葡萄糖注射液)注射於乳牛百會穴以刺激穴位,來探討刺激百會穴對於牛隻黃體功能的影響;另一方面則以低劑量的PGF2α藥物施打於牛隻百會穴,以探討低劑量藥物施打於百會穴對於牛隻黃體溶解的效果。本試驗共使用23頭荷氏登乳牛,以超音波確認於其動情週期的第9到第12天之間進行試驗。試驗組1及對照組1,分別以50%葡萄糖注射液10 ml或生理鹽水注射液10 ml注入百會穴,結果顯示,試驗及對照組在施打水針後72小時內黃體面積及血中progesterone皆無明顯變化,而水針後0-4小時間血中13,14-dihydro-15-keto PGF2α (PGFM)濃度也無明顯變化。試驗組2及對照組2,使用50%劑量(250 μg) PGF2α (EstrumateR, Cloprostenol Sodium)分別注入百會穴或後海穴,結果顯示,給藥後兩組皆造成黃體面積減小,試驗組黃體面積減少的程度雖大於對照組,但兩組面積皆未小於試驗前的50%,此結果與progesterone的變化情形一致。,而試驗組血中PGFM濃度在給藥後0-4小時變化未達顯著差異,但試驗組較對照組有較大的上升幅度。試驗組3及對照組3,使用75%劑量(375 μg)的PGF2α分別注入百會穴或後海穴,結果顯示,試驗組0小時與24小時平均黃體面積之間具有顯著差異(P<0.05),且72小時的平均黃體面積減小至0小時的50.6%,而對照組的平均黃體面積在給藥後72小時內並無明顯的變化。而試驗組3血中progesterone濃度也可見到0小時與24小時平均progesterone濃度之間也具有顯著差異(P<0.05),且72小時的progesterone濃度減低至0小時的48.2%;但對照組在給藥後血中平均progesterone濃度反而上升。總結來說,單獨刺激百會穴未發現有助於黃體溶解的證據,但低劑量PGF2α經由百會穴給藥,比起其他穴位有較強的黃體溶解效果,並具有劑量依賴性與個別牛隻的差異性。因此,當臨床上發生肌肉注射PGF2α效果不良時,可以嘗試使用百會穴的替代給藥途徑。
Endocrine dysfunction altering luteal function, may lead to poor efficiency of extraneous PGF2α to induce luteolysis. Previous studies in aquapuncture using low doses of PGF2α at BaiHui acupoint have been proven to cause luteolysis in mares and cows. Therefore, the objective of the present study was to evaluate the aquapuncture at BAI-HUI acupoint for the induction of luteolysis in dairy cows. The experiment was carried out at the middle stage of estrus cycle (day 9~12) in dairy cows. Each cow with normal cycles was injected with 10 ml of 50% glucose (Group 1) or normal saline (Control 1) at the BAI-HUI acupoint, respectively. Administration of PGF2α (cloprostenol 250 μg, Group 2), (cloprostenol 375 μg, Group 3) was aquapunctured at the BAI-HUI acupoint and HOU-HAI (GV-1) acupoint (cloprostenol 250 μg, Control 2 and cloprostenol 375 μg, Control 3), respectively. In Group 1, no differences in CL area (p=0.352), plasma PGFM concentrations (p=0.974) and plasma progesterone concentrations (p=0.641) were observed among cows treated with 50% glucose and normal saline. In Group 2, the results indicated that the administration of 250 μg PGF2α led to a decreased value of CL area (p=0.207) and plasma progesterone concentration (p=0.719), and an increased plasma PGFM concentration (p=0.362). In Group 3, significant differences were observed in CL regression (p=0.017) and decreased concentration of plasma progesterone (p=0.034) between 0 and 24 hours. However, an increased concentration of plasma PGFM was not significant after 375 μg of PGF2α was administrated. In conclusion, the stimulation of BAI-HUI acupoint with 50% glucose had no improvement on luteolysis in cows, however, administering 375 μg of PGF2α via BAI-HUI acupoint was more efficient than that via other acupuncture points. Furthermore, the effect of PGF2α administered via BAI-HUI acupoint dependent on the dosage of PGF2α and the individual difference of cattle.
URI: http://hdl.handle.net/11455/13105
其他識別: U0005-2907201120500700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2907201120500700
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