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Isolation and charactization of very virluent Marek's disease virus in Taiwan and establishment of its vaccination program
very virluent MDV
|摘要:||採集自疑似雞馬立克氏病(Marek's disease,MD)病雞血液及脾臟病材 ,
接種於雞胚胎纖維芽母細胞及小雞腹腔,共得到 7 株馬立克氏病毒(
Marek's disease virus,MDV)。由 MDV 感染細胞之病變細胞,瓊脂沉降反
Inocula from blood and spleen of Marek's disease (MD) chickens were inoculated into chicken embryo fibroblast (CEF) and abominal cavity of day-old chicks. Seven isolates of Marek's diease virus (MDV) were isolated during the experiment.By the results of cyto- pathic effect, agar gel precipitation and fluorescent antibody tests , 7 isolates were divided into 3 serotypes : serotye 1 of strains LTB-1, LTB-2, LTB-3, LTS-1, LTS-2 ; serotype 2 of strains LTB-4; serotype 3 of strain LTB-5. Strain LTB-1, LTS-1 and Md/5 of the serotype 1 were used to inoculate into the experimental chickens of layers, broilers and six lines of Taiwan local chickens. By the results of pathogenic tests and protection tests of HVT vaccine , strain LTB-1, LTS-1 were considered to be the very virluent MDV (vvMDV). From the results of protection experiments, chickens were ino- culated by monovalent or polyvalent vaccine at 1 day- old,bivalent and trivalent vaccine showed highest efficacy .
|Appears in Collections:||獸醫學系所|
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