Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13306
標題: 牛乳中磺胺劑殘留檢測方法之應用研究及臺灣巿售鮮乳與酪農生乳中磺胺劑殘留之調查
Evaluation of three different analytical methods on detectingidues in milk and a survey of sulfonamide residues in UHT milkn Taiwan
作者: 葉潔怡
Yeh, Chai-i
關鍵字: sulfonamide
磺胺劑
tank milk
TLC
HPLC
ELISA
總乳
薄層層析法
高效液相層析法
酵素連接免疫吸附法
出版社: 獸醫學系
摘要: 本試驗以CITE三種磺胺劑酵素連接免疫吸附試劑套組(CITE Sulfa trio test kit;CSTTK)對牛乳中殘留Sulfa-thiazine(STH)、 Sulfamethazine(SMT)、Sulfadimethoxine(SDM)作快速篩選檢測,發現此 檢測法有下列幾項優點:(1)不需樣品前處理,(2)所需檢測時間只要約7 分鐘,(3)最低檢測感度可達10ppb。本試驗並選取七種常用磺胺劑,結果 發現各組之STH、SMT、及SDM,只要分別都低於1ppm,則不會有干擾的現 象。本試驗為求能由牛奶中同時檢出上述七種磺胺劑,分別在(1)樣品萃 取、 (2)薄層色層分析法(Thin layer chlomatography;TLC)及(3)高效 液相層析法(High performance liquid chlomatography;HPLC)三方面進 行探討。結果在樣品萃取方面,發現牛奶先經二氯甲烷(CH2Cl2)振盪處理 ,再以無水硫酸鈉沈澱蛋白質及脂質,萃取液複經過濾蒸發濃縮,並以甲 醇定容成檢液,其萃取效果頗為良好。至於TLC方面,則以氯仿:異丁醇 =85:15 (v/v)為展開劑,併用螢光發色法可同時檢出8種磺胺劑(上述七 種及Sulfa:SCP)此法的優點為:(1)一次可同時分析10個樣品,(自1994 年8月至1995年6月間,本試驗採用上述三種方法對本省12種品牌的市售鮮 乳進行磺胺劑殘留檢驗,前後共分析樣品213個,結果不合格者 (>0.01ppm)高達64個,其不合格率為30%(64/213);當中以SMT的檢出率最 高(81.2%;52/64),其次分別為SMM(31.2%;20/64)、SDM(17.1%;11/64) 、及STH(1.5%;1/64),其殘留濃度都在0.01-1ppm間。本試驗並分析自 1991年12月至1992年7月間所採集之全省七大酪農總乳樣品336個,結果 有 39個不合格(>0.01ppm),其不合格率達11.6% (39/336)。當中以SMT的 檢出率最高(56.4%;22/39),其次分別是STH(23.0%;9/39)、SMM(17.9% ; 7/39)、SDM(10.2%;4/39)、及SMR(5.1%;2/39),其殘留濃度除1個樣 品之 SMM高達外,其餘都在0.01-1ppm間。牛乳磺胺劑污染情形以夏季比 較嚴重,市售鮮乳和總乳的不合格率分別為57.1%及16.3%,而冬季較輕微 ,市售鮮乳和總乳分別為16.7%及7.5%。
A rapid screening test of STH (Sulfathiazole), SMT (Sulfamethazine), and SDM (Sulfadimethoxine) residues in milk was made by employing a CSTTK (CITE Sulfa Trio test kit) of enzyme linked immunosorbent assay in this study. Advantages of CSTTK assay was found to include: (1) pretreatment of milk sample was not required, (2) result could be obtained within 7 minutes, and (3) minimum detectable concentration for each sulfonamide was as low as 10 ppb. With regard to TLC analysis, the use of By employing the developed screening test (CSTTK and TLC analysis) and quantitative test (HPLC analysis), a survey was made on 213 marketed UHT milk samples of 12 brands collected from August, 1994 to April, 1995. A high violation rate was thus seen reaching approximately 30% (64/213). Among these illegal samples, the appearance rates of sulfonamide residue were SMT, 81.25% (52/64); SMM, 31.2% (20/64); SDM, 17.1% (11/64); and STH, 1.5% (1/64) respectively and concentrations detected were all Contamination of tested milk samples by sulfonamide were more severe in summer than in winter. The summer uncertified rate in market milk, tank milk were 57.1%, 16.3%, respectively. The winter uncertified rate in market milk, tank milk were 16.5%, 7.5%, respectively.
URI: http://hdl.handle.net/11455/13306
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