Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13525
標題: 圈飼雌性台灣水鹿之繁殖性狀及其全年糞孕酮濃度波動之監測
Reproductive performance and monitoring annual pattern of fecal progesterone in farmed female formosan sambar deer (Cervus unicolor swinhoei)
作者: 蔡函穎
Tsai, Han-Ying
關鍵字: Formosan sambar deer
台灣水鹿
Cervus unicolor swinhoei
fecal progesterone
pregnancy diagnosis
ELISA
糞孕酮
妊娠診斷
酵素連結免疫吸附法
出版社: 獸醫學系暨研究所
引用: 許松豪。台灣黑熊糞中性類固醇與其繁殖狀況之研究。碩士論文。國立中興大學獸醫學系。台中市。中華民國。2002。 楊吉宗,毛嘉洪, 張耿瑞,何東輯,詹芳澤。利用糞類固醇激素監測圈養台灣黑熊的繁殖狀態。特有生物研究 8:1-11,2006。 楊錫坤,陳俊吉。舍飼台灣水鹿之生殖及生長性狀與鹿角週期。東海學報 35:187-200,1994。 Adam CL, Moir CE, Atkinson T. Plasma concentrations of progesterone in female red deer (Cervus elaphus) during the breeding season, pregnancy and anoestrus. J Reprod Fertil 74: 631-636, 1985. Asher GW. Oestrous cycle and breeding season of farmed fallow deer, Dama dama. J Reprod Fertil 75: 521-529, 1985. Asher GW, Muir PD, Semiadi G, O''Neill KT, Scott IC, Barry TN. Seasonal patterns of luteal cyclicity in young red deer (Cervus elaphus) and sambar deer (Cervus unicolor). Reprod Fertil Dev 9:587-96, 1997. Asher GW, O''Neill KT, Scott IC, Mockett BG, Fisher MW. Genetic influences on reproduction of female red deer (Cervus elaphus) (1) seasonal luteal cyclicity. Anim Reprod Sci 59: 43-59, 2000. Audigé L, Wilson PR, Morris RS. Reproductive performance of farmed red deer (Cervus elaphus) in New Zealand. I. Descriptive data. Anim Reprod Sci 55: 127-141, 1999. Audigé L, Wilson PR, Morris RS. Risk factors for dystocia in farmed red deer (Cervus elaphus). Aust Vet J 79: 352-357, 2001. Capezzuto A, Chelini MO, Felippe EC, Oliveira CA. Correlation between serum and fecal concentrations of reproductive steroids throughout gestation in goats. Anim Reprod Sci 103: 78-86, 2008. Cook RC, Cook JG, Garrott RA, Irwin LL, Monfort SL. Effects of diet and body condition on fecal progestagen excretion in elk. J Wildl Dis 38: 558-565, 2002. Curlewis JD, Loudon AS, Coleman AP. Oestrous cycles and the breeding season of the Père David''s deer hind (Elaphurus davidianus). J Reprod Fertil 82: 119-126, 1988. Creel S, Christianson D, Liley S, Winnie JA Jr. Predation risk affects reproductive physiology and demography of elk. Science 315: 960, 2007. Crowther JR. Basic principles of ELISA. In: ELISA: theory and practice. Humana Press Inc, Totowa, New Jersey, U.S.A., 35-61, 1995. English AW, Mulley RC. Causes of perinatal mortality in farmed fallow deer (Dama dama). Aust Vet J 69: 191-193, 1992. Flood PF, Tyler NJ, Read EK, Rodway MJ, Chedrese PJ. Ovarian and placental production of progesterone and oestradiol during pregnancy in reindeer. Anim Reprod Sci 85: 147-162, 2005. García A, Landete-Castillejos T, Garde J, Gallego L. Changes in plasma progesterone during pregnancy in Iberian red deer (Cervus elpahus hispanicus). Vet Rec 150: 550-551, 2002a. García AJ, Landete-Castillejos T, Carrión D, Gaspar-López E, Gallego L. Compensatory extension of gestation length with advance of conception in red deer (Cervus elaphus). J Exp Zoolog A Comp Exp Biol 305: 55-61, 2006. García AJ, Landete-Castillejos T, Garde JJ, Gallego L. Reproductive seasonality in female Iberian red deer (Cervus elaphus hispanicus). Theriogenology. 58: 1553-1562, 2002b. Garcia AJ, Landete-Castillejos T, Gomez-Brunet A, Garde JJ, Gallego L. Characteristics of the oestrous cycle of Iberian red deer (Cervus elaphus hispanicus) assessed by progesterone profiles. J Exp Zoolog A Comp Exp Biol 298: 143-149, 2003. Garrott RA, Monfort SL, White PJ, Mashburn KL, Cook JG. One-sample pregnancy diagnosis in elk using fecal steroid metabolites. J Wildl Dis 34: 126-131, 1998. Guinness FE, Albon SD, Clutton-Brock TH. Factors affecting reproduction in red deer (Cervus elaphus) hinds on Rhum. J Reprod Fertil. 54: 325-334, 1978. Haigh JC. The gestation length of wapiti (Cervus elaphus) revisited. Anim Reprod Sci 31: 65: 89-93, 2001. Hamasaki S, Yamauchi K, Ohki T, Murakami M, Takahara Y, Takeuchi Y, Mori Y. Comparison of various reproductive status in Sika deer (Cervus nippon) using fecal steroid analysis. J Vet Med Sci 63: 195-198, 2001. Harder JD, Moorhead DL. Development of corpora lutea and plasma progesterone levels associated with the onset of the breeding season in white-tailed deer (Odocoileus virginianus). Biol Reprod 22: 185-191, 1980. Hirata S, Mori Y. Monitoring reproductive status by fecal progesterone analysis in ruminants. J Vet Med Sci 57: 845-850, 1995. Isobe N, Akita M, Nakao T, Yamashiro H, Kubota H. Pregnancy diagnosis based on the fecal progesterone concentration in beef and dairy heifers and beef cows. Anim Reprod Sci 90: 211-218, 2005a. Isobe N, Nakao T, Yamashiro H, Shimada M. Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle. Anim Reprod Sci 87: 1-10, 2005b. Kapke CA, Arcese P, Ziegler TE, Scheffler GR. Estradiol and progesterone metabolite concentration in white-tailed deer (Odocoileus virginianus) feces. J Zoo Wildl Med 30: 361-371, 1999. Kelly RW, McNatty KP, Moore GH, Ross D, Gibb M. Plasma concentrations of LH, prolactin, oestradiol and progesterone in female red deer (Cervus elaphus) during pregnancy. J Reprod Fertil 64: 475-83, 1982. Korndörfer CM, Meirelles CF, Bueno IC, Abdalla AL. Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay. Braz J Vet Res Anim Sci 35: 115-119, 1998. Kornmatitsuk B, Thitaram C, Kornmatitsuk S. Measurement of faecal progesterone metabolites and its application for early screening of open cows post-insemination. Reprod Domest Anim 42: 238-242, 2007. Li C, Jiang Z, Jiang G, Fang J. Seasonal changes of reproductive behavior and fecal steroid concentrations in Père David''s deer. Horm Behav. 40: 518-25, 2001. Liu BT, Cheng SP, Huang MC, Yu JY. Serum progesterone changes in luteal cyclicity and duration of estrous cycle in Formosan sika deer (Cervus nippon taiouanus) hinds. Zoolog Sci 19: 1033-1037, 2002. Martin F, Peltonen J, Laatikainen T, Pulkkinen M, Adlercreutz H. Excretion of progesterone metabolites and estriol in faeces from pregnant women during ampicillin administration. J Steroid Biochem 6: 1339-1346, 1975. Martin F, Peltonen J, Laatikainen T, Tikkanen M, Pulkkinen M. Excretion of unconjugated and conjugated progesterone metabolites in pregnancy urine during ampicillin administration. Clin Chim Acta 55: 71-80, 1974. Matsuura Y, Sasamoto Y, Sato K, Takahashi Y, Suzuki M, Ohtaishi N. Monitoring ovarian cycle and conception by fecal progesterone analysis in sika deer. Ecological Research 19: 397-404, 2004. Mattiello S, Mattiangeli V, Bianchi L, Carenzi C. Feeding and social behavior of fallow deer (Dama dama L.) under intensive pasture confinement. J Anim Sci 75: 339-347, 1997. Mauget R, Mauget C, Dubost G, Charron F, Courcoul A, Rodier A. Non-invasive assessment of reproductive status in Chinese water deer (Hydropotes inermis): Correlation with sexual behaviour. Mamm biol 72: 14–26, 2007. McLeod BJ, Haresign W. Evidence that progesterone may influence subsequent luteal function in the ewe by modulating preovulatory follicle development. J Reprod Fertil 71: 381-386, 1984. Morrow CJ, Monfort SL. Ovarian activity in the scimitar-horned oryx (Oryx dammah) determined by faecal steroid analysis. Anim Reprod Sci 53: 191-207, 1998. Mylrea GE, Mulley RC, English AW, Evans G. Reproductive cycles of farmed female chital deer (Axis axis). Reprod Fertil Dev 11: 167-173, 1999. Noakes DE. Parturition and the care of parturient animals. In: Noakes DE, Parkinson TJ, England GCW, ed. Arthur’s veterinary reproduction and obstetrics, 8th ed. Saunders, Philadelphia, U.S.A., 158, 2001. Osborn DA, Gassett JW, Miller KV, Lance WR. Out-of-season breeding of captive white-tailed deer. Theriogenology 54: 611-619, 2000. Palme R, Fischer P, Schildorfer H, Ismail MN. Excretion of infused 14C-steroid hormones via faeces and urine in domestic livestock. Anim Reprod Sci 43: 43-63, 1996. Parra MD, García AJ, Bernal LJ, Landete-Castillejos T, Cerón JJ. Progesterone determination in Iberian red deer by time-resolved fluorometry: an alternative method to RIA. J Exp Zoolog A Comp Exp Biol 301: 472-476, 2004. Pereira RJ, Polegato BF, de Souza S, Negrão JA, Duarte JM. Monitoring ovarian cycles and pregnancy in brown brocket deer (Mazama gouazoubira) by measurement of fecal progesterone metabolites. Theriogenology 65: 387-399, 2006. Plotka ED, Seal US, Schmoller GC, Karns PD, Keenlyne KD. Reproductive steroids in the white-tailed deer (Odocoileus virginianus borealis). I. Seasonal changes in the female. Biol Reprod 16: 340-343, 1977a. Plotka ED, Seal US, Verme LJ, Ozoga JJ. Reproductive steroids in the white-tailed deer (Odocoileus virginianus borealis). II. Progesterone and estrogen levels in peripheral plasma during pregnancy. Biol Reprod 17: 78-83, 1977b. Qureshi MS, Habib G, Nawab G, Siddiwqui MM, Ahmad N, Samad HA. Milk progesterone profiles in various reproductive states in dairy buffaloes under field conditions. Proc Natl Sci Counc Repub China B 24: 70-75, 2000. Rabiee AR, Macmillan KL, Schwarzenberger F. The effect of level of feed intake on progesterone clearance rate by measuring faecal progesterone metabolites in grazing dairy cows. Anim Reprod Sci 67: 205-214, 2001. Rioux P, Rajotte D. Progesterone in milk: a simple experiment illustrating the estrous cycle and enzyme immunoassay. Adv Physiol Educ 28: 64-67, 2004. Roffe TJ. Perinatal mortality in caribou from the Porcupine herd, Alaska. J Wildl Dis 29: 295-303, 1993. Ropstad E, Veiberg V, Säkkinen H, Dahl E, Kindahl H, Holand O, Beckers JF, Eloranta E. Endocrinology of pregnancy and early pregnancy detection by reproductive hormones in reindeer (Rangifer tarandus tarandus). Theriogenology 63: 1775-1788, 2005. Sanders H, Rajamahendran R, Burton B. The development of a simple fecal immunoreactive progestin assay to monitor reproductive function in swine. Can Vet J 35: 355-358, 1994. Schwarzenberger F. The many uses of non-invasive faecal steroid monitoring in zoo and wildlife species. Int Zoo Yb 41: 52-74, 2007. Semiadi, G, Barry, TN, Muir PD. General biology of sambar deer (Cervus unicolor) in captivity. New Zealand Jour Agric Res 37: 79-85, 1994. Shipka MP, Rowell JE, Ford SP. Reindeer bull introduction affects the onset of the breeding season. Anim Reprod Sci 72: 27-35, 2002. Shipka MP, Rowell JE, Sousa MC. Steroid hormone secretion during the ovulatory cycle and pregnancy in farmed Alaskan reindeer. J Anim Sci 85: 944-951, 2007. Stoops MA, Anderson GB, Lasley BL, Shideler SE. Use of Fecal Steroid Metabolites to estimate the pregnancy rate of a free-ranging herd of tule Elk. J Wildl Manage 63: 561-569, 1999. Stewart-Scott IA, Pearce PD, Moore GH, Fennessy PF. Freemartinism in red deer (Cervus elaphus L.). Cytogenet Cell Genet 54: 58-59, 1990. Suzuki M, Ohtaishi N. Reproduction of female Sika deer (Cervus nippon yesoensis Heude, 1884) in Ashoro District, Hokkaido. J Vet Med Sci 55: 833-836, 1993. Takahashi T, Hamanaka S, Imai K, Hashizume K. Fecal progesterone analysis by time-resolved fluoroimmunoassay (TR-FIA) for monitoring of luteal function in the sika doe (Cervus nippon centralis). J Vet Med Sci 64: 565-569, 2002. Thompson KV, Mashburn KL, Monfort SL. Characterization of estrous cyclicity in the sable antelope (Hippotragus niger) through fecal progestagen monitoring. Gen Comp Endocrinol 112: 129-137, 1998. Yamauchi K, Hamasaki S, Miyazaki K, Kikusui T, Takeuchi Y, Mori Y. Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon). J Vet Med Sci 62: 669-671, 2000. Yamauchi K, Hamasaki S, Takeuchi Y, Mori Y. Application of enzyme immunoassay to fecal steroid analysis in sika deer (Cervus nippon). J Reprod Dev 45: 429-434, 1999. Yamauchi K, Hamasaki S, Takeuchi Y, Mori Y. Assessment of reproductive status of sika deer by fecal steroid analysis. J Reprod Dev 43: 221-226, 1997. http://edb.epa.gov.tw/localenvdb/Nantou/climate.htm http://www.cwb.gov.tw/
摘要: 台灣水鹿(Cervus unicolor swinhoei)為台灣特有亞種,本研究之目的在調查雌性台灣水鹿之繁殖性狀表現,第二部分為分析其全年糞孕酮之濃度變化。於本研究中從2000至2008年間共有525筆以上的繁殖紀錄,包含動情週期、懷孕期和產後之情形。繁殖紀錄資料分析結果顯示,雌性台灣水鹿之動情週期長度平均為18.2 ± 0.5日,範圍為10 - 25日。懷孕期長度、分娩間距和哺乳期長度之平均值則分別為259.6 ± 0.5日、369.6 ± 2.3日和81.9 ± 1.3日,表示雌性台灣水鹿每年平均可生產一胎次。依仔鹿性別計算母鹿懷雄性仔鹿和雌性仔鹿之懷孕期平均值分別為259.7 ± 0.6日和259.9 ± 0.8日,懷孕期長度不受仔鹿性別和雙胞胎之影響。每年6月至12月皆有母鹿的配種紀錄,其中93.1%(460/494)集中於7至10月配種,且達顯著性差異。每年2至9月皆有母鹿分娩之紀錄,其中83.1%(261/314)的分娩集中於4至6月,且達顯著性差異。此外,317筆分娩紀錄中,僅出現一對異卵雙胞胎,其發生率為0.32%。出生仔鹿之公母比為1.3 : 1,雄性仔鹿與雌性仔鹿之出生性別並非均等比例分佈,且雄性仔鹿較雌性仔鹿有較高的性別別死亡率(6.7% vs. 5.8%),但不具有顯著性差異。第二部分的實驗動物分別為南投縣A鹿場的11頭母鹿和B鹿場的6頭母鹿,由2007年1月1日至12月31日止,每週收集其糞便3次,利用酵素連結免疫吸附法測定其糞孕酮之濃度。實驗結果顯示,A鹿場之糞孕酮濃度大於380 ng/g視為懷孕,B鹿場之糞孕酮濃度大於130 ng/g視為懷孕。A鹿場於配種後36至44日妊娠診斷之敏感度和特異度分別為83.3 - 100% 和 100%,而B鹿場則為100% 和100%。A鹿場之糞孕酮濃度於繁殖各期(懷孕早期、中期和晚期,產後期、哺乳期和產後發情期)皆顯著地高於B鹿場。以重複測量分析懷孕週數(x)與糞孕酮濃度(y)之線性關係,於A和B鹿場分別為y = 206.30 x和y = 80.17 x,且懷孕期之糞孕酮濃度不受母鹿之年齡、胎次或仔鹿之性別影響。此外,於9頭母鹿在分娩前6.7 ± 2.4日可觀察到糞孕酮濃度顯著地下降。因此,本實驗結果顯示,糞孕酮分析法可成功應用雌性台灣水鹿於懷孕全期糞孕酮濃度之監測,並可作為早期妊娠診斷和預測分娩之指標。
Formosan sambar deer (FSD, Cervus unicolor swinhoei) is a native subspecies in Taiwan and its reproductive traits remains unclear. The aim of this study was to investigate the reproductive performance of female FSD throughout the year, and secondly, to analyze their annual pattern of fecal progesterone. A total of 210 adult female FSD in four deer farms in Nantou County were used for this investigation. Extensive analyses of 525 reproductive records, including the conditions of estrus, gestation, and parturition from these hinds, were collected from 2000 to 2008. The results indicated that the mean estrus cycle was 18.2 0.5 days (n = 56, varying from 10 to 25 days). The mean length of gestation (n = 288), calving interval (n = 122), and length of lactation (n = 87) were 259.6 0.5 days, 369.9 2.3 days, and 81.9 1.3 days, respectively, indicating the characteristic of annual single parity in female FSD. The average lengths of gestation for male and female calves were 259.7 0.6 days (n = 159) and 259.9 0.8 days (n = 129), respectively. The length of gestation was not significantly affected by the gender of calves or twin birth. Rutting occurred from June to December (n = 494), with a peak in July/October (n = 460) (p < 0.05). Calving distributed from February to September (n = 314), with a peak in April/June (n = 261) (p < 0.05). Only one twin birth was documented in the 317 calving data (0.32%). The male/female ratio of calves (1.3:1, n = 318) differed significantly from the equal ratio (p < 0.05). Male calves had a higher gender-specific mortality rate after birth than the females (6.7% vs. 5.8%). In additions, fecal samples of 11 hinds from the Farm A and of 6 hinds from the Farm B in Nantou County were collected 3 times a week from Jan. 01 to Dec. 31, 2007, to determine their fecal progesterone (FP4) concentrations by enzyme-linked immunosorbent assay (ELISA). The hinds were considered pregnant when FP4 > 380 ng/g in the Farm A and FP4 > 130 ng/g in the Farm B, respectively. The sensitivity and specificity for the pregnancy diagnosis of hinds on days 36 - 44 after copulation were 83.3 - 100% and 100% in the Farm A, while these were 100% and 100% in the Farm B. The mean FP4 concentrations of the hinds in the different reproductive phases, including the periods of early, middle and late pregnancy, postpartum, lactation and postpartum estrus in Farm A were higher than these in Farm B (p < 0.05). The analysis of repeated measures showed that the linear model was useful to predict significantly FP4 concentrations from weeks of gestation with y = 206.30 x for Farm A (r2 = 0.5002, p < 0.05) and y = 80.17 x for Farm B (r2 = 0.2575, p < 0.05). The FP4 value of hinds in gestation was neither significantly affected by the age and parity of hinds, nor the sex of calves. Besides, the levels of FP4 started a marked decline 6.7 2.4 days prior to parturition in 9 pregnant hinds (p < 0.05). In the present study, the FP4 analysis was successfully used for non-invasive monitoring of FP4 fluctuation during the pregnancy, early pregnancy diagnosis and the prediction of calving in farmed female FSD.
URI: http://hdl.handle.net/11455/13525
其他識別: U0005-1007200817123400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1007200817123400
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