Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13681
標題: 以PCR技術調查台灣乳牛焦蟲症及邊蟲症之流行病學
Epidemiological Study of Bovine Babesiosis and Anaplasmosis by PCR-based technology in Taiwan
作者: 林怡孜
Lin, I-Tzu
關鍵字: B. bovis
邊蟲症
B. bigemina
A. marginale
PCR
聚合酶鏈鎖反應
出版社: 獸醫學系暨研究所
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摘要: 乳牛焦蟲症(babesiosis)及邊蟲症(anaplasmosis)均是牛隻非常重要的壁蝨媒介性疾病(tick-borne diseases),在熱帶及亞熱帶國家造成非常嚴重的經濟損失。造成乳牛焦蟲症最主要的焦蟲種類(Babesia spp.)為B. bovis及B. bigemina,它們主要是由一些Rhipicephalus屬的硬蜱所傳播。而造成乳牛邊蟲症的主要病原為Anaplasma marginale,它主要是由吸血節肢動物生物性或機械性的傳播。本實驗目的乃欲利用高敏感性及高特異性的PCR技術偵測B. bovis、B. bigemina及A. marginale,調查台灣泌乳牛焦蟲症及邊蟲症的流行病學特性。在台灣總共進行12個牧場的採樣工作,共蒐集了520個血液樣本。其中一個在北台灣的牧場,在採樣期間爆發焦蟲症,於牛隻身上共採集到8隻壁蝨,鑑定皆為Rhipicephalus microplus。比較診斷焦蟲症的PCR方法,nested-PCR與duplex nPCR比single-step PCR有較高之陽性檢出率,具統計學顯著差異 (P<0.05)。另外,只有一個在南台灣曾感染過R. microplus的牧場,以nPCR有檢測出B. bovis及B. bigemina(檢出率分別為12/46,26.1%及2/46,4.3%)。在邊蟲症的流行病學方面,採樣的12個牧場皆有檢測出A. marginale,盛行率為53.3%(277/520);比較各地區的盛行率,南部地區的盛行率(83.9%)明顯高於其他地區(北、中及東部地區分別為44.6%、42.5%及47.8%),地區間具有顯著差異(P<0.05)。比較焦蟲症及邊蟲症的盛行率,邊蟲症的盛行率(53.3%)顯著高於焦蟲症(B. bovis及B. bigemina,3.1%)的盛行率。根據本研究結果,我們可得到結論,邊蟲是台灣牧場中普遍存在的血液寄生蟲,而焦蟲症的感染分佈則與R. microplus的感染有關。
Bovine babesiosis and anaplasmosis are important tick-borne diseases that results in economic losses in tropical and subtropical regions. The two most important species of Babesia in cattle are B. bovis and B. bigemina which are mainly transmitted by some species of Rhipicephalus genus ticks. The main Anaplasma species in cattle is Anaplasma marginale which is mainly transmitted mechanically and/or biologically by arthropod. We tried to apply PCR-based methods with high sensitivity and specificity to detect B. bovis, B. bigemina, and A. marginale to survey epidemiology of babesiosis and anaplasmosis in Taiwan. A total of 520 blood samples from 12 dairy farms were collected. One of these farms in north Taiwan during the experiemental period had an outbreak of babesiosis. A total of 8 ticks were collected in that farm and all were identified as Rhipicephalus microplus. Comparison of sensitivity with single-step PCR, nested-PCR and duplex nested-PCR for diagnosis of babesiosis, nested-PCR and duplex nested-PCR were significantly more sensitive than single-step PCR (P<0.05). Morever, in another farm with a history of R. microplus infestation in south Taiwan, it was demonstracted by nested-PCR that B. bovis and B. bigemina infection in the cattle were 26.1% (12/46) and 4.3% (2/46), respectively. A. marginale was detected from all sampling farms with the prevalence of 53.3% (277/520). Comparison of different regions, showed south Taiwan (83.9%) was significantly higher prevalence of anaplasmosis than north (44.6%), middle (42.5%), and east (47.8%) Taiwan (P<0.05). The prevalence of anaplasmosis (53.3%) was significantly higher than babesiosis (B. bovis and B. bigemina, 3.1% among the 11 investigated faarms). We concluded that anaplasmosis is a common hemoparasite disease in Taiwan farms and babesiosis correlates closely with R. microplus.
URI: http://hdl.handle.net/11455/13681
其他識別: U0005-3007200816155000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-3007200816155000
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