Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13769
標題: DEVELOPMENT AND EVALUATION OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA ) FOR DETECTING AVIAN SALMONELLOSIS
作者: 盧文鴻
Lu, Wen-Hong
關鍵字: AVIAN SALMONELLOSIS
間接酵素連結免疫
LIPOPOLYSACCHARIDE ANTIGEN
FLAGELL
ANTIGEN
VETERINARY
ANIMAL-SCIENCE
家禽沙氏桿菌症
脂多醣抗原
鞭毛抗原
獸醫
動物學
出版社: 獸醫研究所
摘要: 以三種 LPS 抗原檢測 900 個種雞血清樣本, S.pullorum LPS 抗原單 獨檢出陽性比例為 7 ﹪ (63/900) , S.enteritidis LPS 抗原單獨檢 出陽性比例為 5.2 ﹪ (47/900) , S.typhimurium LPS 抗原單獨檢出 陽性比例為 0.22 ﹪ (2/900) , 其中 S.pullorum LPS 抗原與 S. enteritidis LPS 抗原的重複檢出陽性比例高達 75 ﹪ (675/900) , S. pullorum LPS 抗原與 S.typhimurium LPS 抗原重複檢出陽性比例為 31.67 ﹪ (285/900) , S.enteritidis LPS 抗原與 S.typhimurium LPS 抗原重複檢出陽性比例為 31.56 ﹪ (284/900) 。 由此可見,同屬 於 D1 血清群的 S.pullorum 和 S.enteritidis LPS 抗原彼此間有較明 顯的交叉反應產生, 而與屬於 B 血清群的 S.typhimurium LPS 抗原之 間的交叉反應則較不明顯。 三種 LPS 抗原重複檢出陽性的 282 個血清 樣本, 以兩種鞭毛抗原篩檢結果, S.enteritidis 和 S.typhimurium 鞭毛抗原檢出陽性率分別為 95.39 ﹪ (269/282) 、 94.33 ﹪ (266/282) ,重複檢出率為 91.84 ﹪ (259/ 282) ,顯示鞭毛抗原之間 的交叉反應十分明顯。 When the estabished ELISA antigen plates were used to detect 900 field serum samples ,675 out of 900(75 ﹪ )were positive in both S.pullorum and S.enteritidis lipopolysaccharide antigens (LPS Ag) , 285 out of 900(31.67 ﹪ ) were positive in both S. pullorum and S. typhimurum LPS Ag and 31.56 ﹪ (284/900) in both S.enteritidis and S.typhimurium LPS Ag, whereas 63 out of 900 (7 ﹪ ) were positive in S.pullorum LPS Ag only, 47 out of 900 (5.2 ﹪ ) in S.enteritidis LPS Ag only.It was noted that the rate of cross reaction within the same group. There were 282 serum samples which were ELISA positive in all three kinds of lipopolysaccharide antigen. Those sera were subjected to the ELISA tests using flagella antigen from S. enteritidis or S.typhimurium. the positive rate was 95.39 ﹪ (269/ 282) in S.enteritidis flagella antigen only, 94.33 ﹪ (266/282) in S.typhimurium flagella antigen only, and 91.84 ﹪ (259/282) in both flagella antigen. It is evident that there are strong cross reaction in ELISA tests using flagella antigens.
URI: http://hdl.handle.net/11455/13769
Appears in Collections:獸醫學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.