Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13794
標題: 以巢式聚合酶鏈鎖反應調查台灣反芻動物Q熱之流行病學
Use of Nested-PCR to Investigate the Epidemiology of Q fever in Ruminants in Taiwan
作者: 池有容
Chih, Yu-Jung
關鍵字: Q fever
巢式聚合酶鏈鎖反應
Nested-PCR
Ruminants
Epidemiology
反芻動物
流行病學
出版社: 獸醫學系暨研究所
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摘要: Q fever是一種廣泛分佈於世界的人畜共通傳染病,由立克次體病原Coxiella burnetii所引起,此疾病具有造成大規模急性感染的潛在危險。家畜動物如牛、山羊及綿羊目前被認為是人類最重要感染來源。不同於動物感染Q fever後通常無明顯臨床症狀,僅偶爾引起流產或繁殖障礙,Coxiella burnetii在人類造成嚴重疾病與慢性心內膜炎。本實驗目的欲使用具有高度敏感度及特異度的巢式聚合酶鏈鎖反應(nested-PCR)技術,調查台灣反芻類家畜動物Q fever的流行病學,並評估分別針對C. burnetii的com1基因及IS1111基因為標的之兩組引子對,對於病原能力是否有差異。自2007年5月起至2009年2月,在台灣15個牛場及3個山羊場,共採集574個牛血液及134個山羊血液樣本,以及11個流產檢體及72個陰道拭子樣本。以兩組引子對檢測標準菌株DNA之敏感度皆為1 pg/μl。在全部791個經nested-PCR檢測的檢體,共有9個檢體檢測出IS1111基因,然而僅有1個檢體檢測出com1基因。陽性檢體在各採樣分類中的陽性率,以山羊流產胎盤檢體的60% (3/5)為最高、其次為山羊流產胎兒組織檢體25% (1/4)、山羊陰道拭子6.25% (4/64)。牛及山羊血液樣本的陽性率分別為0.2% (1/574)與0%。另外年齡及地理位置因子與陽性率皆無顯著相關。依據本實驗結果,以nested-PCR方式針對IS1111基因所設計之引子對來檢測C. burnetii,相較於com1基因有較高的敏感度,並適合用於實驗室Q fever的診斷。台灣反芻動物的C. burnetii陽性率相對較低,然而山羊流產檢體如胎盤及胎兒有較高的陽性檢出率分別為60%及25%,顯示接觸此類流產產物可能為人類感染重要危險因子,建議畜牧相關人員在處理此類檢體時應特別謹慎。
Q fever is a ubiquitous zoonosis caused by Coxiella burnetii which has the potential risk to cause large outbreaks of acute infection. Domestic animals, such as cattle, goat, and sheep, have been considered to be the most important source of transmission to humans. Whereas most of infected animals show no clinical signs except for occasional abortions or other reproductive anomalies, C. burnetii can cause severe illness and chronic endocarditis in human. The aim of the present study was to apply nested polymerase chain reactions (nested-PCR) with high sensitivity and specificity to investigate the epidemiology of Q fever in ruminants in Taiwan, and to compare the use of two different nested-PCR targeting; com1 and IS1111 gene for C. burnetii identification. A total of 574 bovine and 134 caprine blood samples were collected from 15 cattle farms and 3 goat farms from May 2007 to February 2009. Moreover, 11 aborted specimens and 72 vaginal swabs were also collected in this study. The sensitivity of both primers were 1 pg/μl when detecting standard DNA sample. From all of 791 specimens tested by nested-PCR, 9 were positive for IS1111, with only 1 positive result for com1. Aborted placenta from does had the highest positive rate 60% (3/5), followed by aborted fetus 25% (1/4) and vaginal swabs 6.25% (4/64). The prevalence in cattle and goat blood samples were 0.2% and 0% respectively. By chi-square test, there was no significant difference between the positive rate by age and geographic distribution. In conclusion, the results of this study suggest that the nested-PCR with primers targeted to the IS1111 gene is more sensitive than primers targeted to the com1 gene for the detection of C. burnetii, and nested-PCR is a useful tool for laboratory Q fever diagnosis. In Taiwan, the prevalence rate of C. burnetii was relatively low in ruminants. Aborted specimens, such as placenta and fetus form does, revealed a high percentage of positive results (60% and 25%, respectively) which may indicate that contact with those genital products from aborted animal could be an important risk factor of human infection.
URI: http://hdl.handle.net/11455/13794
其他識別: U0005-1308200916544700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1308200916544700
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