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標題: 在不同濃度鹼性纖維母細胞生長因子下脈衝電磁場對大鼠骨髓基質細胞之影響
Effects of Pulsed Electromagnetic Fields on Rat Bone Marrow Stromal Cell in Different Concentration of Basic Fibroblast Growth Factor
作者: 張佳玲
Chang, Jia-Ling
關鍵字: 脈衝電磁場
出版社: 獸醫學系暨研究所
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摘要: Pulsed electromagnetic fields have been used in orthopedic disease for thirty years. Bone marrow stromal cells (BMSCs) are multipotent stem cell. In our preliminary study, some researchers found that it seemed that there was no effect when BMSCs were simply exposed to PEMFs. For the reason given above, we have adequate reason to think that BMSCs may need some inducers to become sensitive to PEMFs. Basic fibroblast growth factor (bFGF) is important in the processes of proliferation and differentiation. In the present study, according to different concentrations of bFGF (0, 5, 10 ng/ml) in medium, we divided BMSCs into 3 groups. BMSCs were set in a microincubator between a pair of Helmholtz coils powered by a pulse generator (60 Hz, 25 gauss) for 6 hours a day for a period of 4 days. By using immunochemical staining methods and MTT assay, we found that PEMFs can promote the proliferation and viability of cells, especially in the group that contained bFGF (5 ng/ml). The results of proliferation and viability are similar in bFGF 5 and 10 ng/ml plus PEMFs. To probe into intracellular signal transduction, we used inhibitors which correlated with cell proliferation. The results indicate that treatment with Genistein(protein tyrosine kinase inhibitor) block the effect of PEMFs but not bFGF. Furthermore, another results indicate that treatment with either PD098059 or U0126 (both are MEK inhibitors) block the effect of PEMFs. It means that PEMFs and bFGF seem to enhance cell proliferation and viability via the MEK pathway and have a synergistic effect. We hope the application of PEMFs/bFGF combined with BMSCs transplantation in vivo to enhance cells viability and proliferation in the future. Comparatively, the synergistic effect of PEMFs and bFGF may be worthy to explore.
其他識別: U0005-2407200911391500
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