Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/13986
標題: 台灣地區2007-2008年間分離禽源新城病毒之特性
Characterization of Newcastle Disease Virus Isolated from Poultry in Taiwan during 2007-2008
作者: 陳玉林
Chen, Yu Lin
關鍵字: Newcastle disease virus
新城病
ICPI
waterfowl
transmission
基因型


保毒者
出版社: 獸醫學系暨研究所
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摘要: 在台灣2007-2008年間,於雞、鴿與鴨樣本上總共分離出六株新城病病毒,其中有四株病毒株來自於雞,其命名為TW/07-1113、TW/08-1023、TW/08-1123與TW/08-1203,一株病毒株來源於鴿子,稱TW/08-1017P,與一株來源於鴨,稱TW/08-83D。利用胚胎蛋致死時間、腦內接種致病指數與核苷酸序列的等方法分析所有病毒株,其中除了TW/08-83D為弱毒株外,其餘五株皆為中強毒株,其中又以TW/08-1023、TW/08-1123與TW/08-1203三株病毒株之核苷酸與胺基酸序列相似度最高,根據此結果推測此三株病毒株應為同一來源。此外,利用TW/07-1113雞源NDV強毒株接種107.2 EID50/0.2mL於小鵝與小鴨,於實驗期間水禽皆無出現臨床症狀與死亡之情形,以RT-PCR可檢測出臟器具有病毒核酸的存在,且以淋巴組織器官所偵測的21天為最久,但僅能於攻毒後五天內可再分離出病毒,除此之外,配合於攻毒後3天所放入之SPF雞隻 (Group1)混合飼養的結果,可見水禽在感染雞源NDV強毒株後確實可以排毒並傳染給雞隻,不過由於在攻毒後17天所混合飼養的雞隻 (Group2),並沒有出現有新城病之致病情形,顯示NDV病毒感染水禽僅能在攻毒早期產生體內短暫的複製與排毒。另外在2008年間,由326個水禽共泄腔拭子以RT-PCR監控病毒核酸之結果,其陽性率達20.5%,再配合血清樣本以血球凝集抑制試驗結果,陽性率達80.3% (424/528),顯示水禽類在台灣的環境中,確實有NDV的感染。由於水禽類可以做為NDV的保毒者,因此必須 更加重視水禽類在NDV傳播上所扮演的角色。
Six isolates of Newcastle disease virus (NDV) were isolated from chickens, pigeons and ducks in Taiwan during 2007-2008. Four of six isolates were isolated from chicken, designed TW/07-1113、TW/08-1023、TW/08-1123 and TW/08-1203 respectively. One was isolated from pigeons, designed TW08-1017P, the other was isolated from ducks, designed TW08-83D. We characterized the six isolates by mean death time (MDT) in eggs, intracerebral pathogenicity indexes (ICPI) and nucleotide sequence analysis. Pathogenicity test showed that all isolates expect one (TW/08-83D) were belonged mesogenic and velogenic strain. We compared the nucleotide and amino acid sequences of six isolates and found that three of chicken-origin NDV, TW/08-1023、TW/08-1123 and TW/08-1203, were highly homologious. Based on this result, we concluded that the three chicken-origin NDV strains were the same ancestor. Furthermore, we used 107.2 egg infectious dose (EID50)/0.2mL chicken-origin NDV (TW/07-1113) to challenge goslings and ducklings. Waterfowls did not show any clinical signs and death during the experiment period but we could detect the viral RNA in organs of waterfowls by RT-PCR. Moreover, the longest period of detection was 21 days in lymphoid tissues. The virus also could be recovered within the fifth day post inoculation. In addition, the results that specific pathogen free (SPF) chicken (Group1) were mixed feeding in 3 day post inoculation were confirmed waterfowls could infect chicken-origin NDV and transmitted the virus to in-contact chicken. However, the SPF chicken (Group2) that mixed feeding in 17 day post inoculation did not cause pathogenecity of Newcastle disease. This result suggested that waterfowls could be infected by virulent NDV, and the virus can replicate in vivo and shed virus in early inoculation. On the other hand, we screened 356 cloacal swabs to detect NDV by RT-PCR in 2008 and the positive rate of RT-PCR was 20.5%. In the serological survey results, we examined 528 sera for the presence of NDV antibodies and the positive rate was 80.3% (424/528). Based on this result, we conclude that there is NDV infection in waterfowls in Taiwan and waterfowls can be a reservoir of NDV. Therefore, waterfowls play animportant role in NDV transmission.
URI: http://hdl.handle.net/11455/13986
其他識別: U0005-2707200919333800
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