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標題: 犬貓乳腺腫瘤類似小鼠乳腺腫瘤病毒序列之偵測
Investigation of Mouse Mammary Tumor Virus (MMTV)-Like Gene Sequences in Canine and Feline Mammary Tumors
作者: 林杏怡
Lin, Hsing-Yi
關鍵字: MMTV
breast cancer

出版社: 獸醫學系暨研究所
引用: Acha-Orbea H, Palmer E. Mls- a retrovirus exploits the immune system. Immunol Today.12:356-361, 1991. Allred DC, Harvey JM, Berardo M, Clark GM. Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol. 11:155-68, 1998. Bhadra S, Lozano MM, Dudley JP. Conversion of mouse mammary tumor virus to a lymphomagenic virus. J Virol. 79:12592-12596, 2005. Bindra A, Muradrasoli S, Kisekka R, Nordgren H, Warnberg F, Blomberg J. Search for DNA of exogenous mouse mammary tumor virus-related virus in human breast cancer samples. J Gen Virol. 88:1806-1809, 2007. Bittner JJ. Some possible effects of nursing on the mammary tumor incidence in mice. Science. 84:162-169, 1936. Bittner JJ. The milk influence of breast tumor in mice. Science. 95:462-463, 1942. Broussard DR, Mertz JA, Lozano M, Dudley JP. Selection for cmyc integration sites in polyclonal T-cell lymphomas. J Virol. 76:2087-2099, 2000. Burzyn D, Rassa JC, Kim D, Nepomnaschy I, Ross SR, Piazzon I. Toll-like receptor 4-dependent activation of dendritic cells by a retrovirus. J Virol. 78:576-584, 2004. Chang CC, Tsai MH., Liao JW, Chan JP-W, Wong ML, Chang SC. Evaluation of hormone receptor expression for use in predicting survival of female dogs with malignant mammary gland tumors, J Am Vet Med Assoc. 235: (in press), 2009. Choi Y, Kappler JW, Marrack R. A superantigen encoded in the open reading frame of the 3'' long terminal repeat of the mouse mammary tumor virus. Nature. 350: 203- 207, 1991. Cohen JC, Varmus HE. Endogenous mammary tumour virus DNA varies among wild mice and segregates during inbreeding. Nature. 278:418-423, 1979. Courreges MC, Burzyn D, Nepomnaschy I, Piazzon I, Ross SR. Critical role of dendritic cells in mouse mammary tumor virus in vivo infection. J Virol. 81: 3769-3777, 2007. Dion AS, Girardi AJ, Williams CC, Pomenti AA, Redfield ES. Responses of serum from breast cancer patients to murine mammary tumour virus: fact or artefact? J Natl Cancer Inst. 79:207-211, 1987. Etkind P, Du J, Khan A, Pillitteri J, Wiernik PH. Mouse mammary tumor virus-like env gene sequences in human breast tumors and in a lymphoma of a breast cancer patient. Clin Cancer Res. 6:1273-1278, 2000. Etkind PR, Stewart AF, Dorai T, Purcell DJ, Wiernik PH. Clonal isolation of different strains of mouse mammary tumor virus-like DNA sequences from both the breast tumors and non-Hodgkin's lymphomas of individual patients diagnosed with both malignancies. Clin Cancer Res. 10:5656-5664, 2004. Etkind PR, Stewart AF, Wiernik PH. Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter. Infect Agent Cancer. 3: 1-11, 2008. Faedo M, Ford CE, Mehta R, Blazek K, Rawlinson WD. Mouse mammary tumor-like virus is associated with p53 nuclear accumulation and progesterone receptor positivity but not estrogen positivity in human female breast cancer. Clin Cancer Res. 10:4417-4419, 2004. Faschinger A, Rouault F, Sollner J, Lukas A, Salmons B, Indik S. Mouse mammary tumor virus integration site selection in human and mouse genomes. J Virol. 82:1360-1367, 2008. Finke D, Acha-Orbea H. Differential migration of in vivo primed B and T lymphocytes to lymphoid and non-lymphoid organs. Eur J Immunol. 31:2603-2611, 2001. Ford CE, Faedo M, Crouch R, Lawson JS, Rawlinson WD. Progression from normal breast pathology to breast cancer is associated with increasing prevalence of mouse mammary tumor virus-like sequences in men and women. Cancer Res. 64:4755-4759, 2004a. Ford C, Faedo M, Delprado W, Rawlinson W. Mouse mammary tumor virus-like RNA transcripts and DNA are found in affected cells of human breast cancer. Clin Cancer Res. 10:7284-7289, 2004b. Ford CE, Tran D, Deng Y, Ta VT, Rawlinson WD, Lawson JS. Mouse mammary tumor virus-like gene sequences in breast tumors of Australian and Vietnamese women. Clin Cancer Res. 9:1118-1120, 2003. Frank O, Verbeke C, Schwarz N, Mayer J, Fabarius A, Hehlmann R, Leib-Mosch C, Seifarth W. Variable transcriptional activity of endogenous retroviruses in human breast cancer. J Virol. 82:1808-1818, 2008. Fukuoka H, Moriuchi M, Yano H, Nagayasu T, Moriuchi H. No association of mouse mammary tumor virus-related retrovirus with Japanese cases of breast cancer. J Med Virol. 80:1447-51, 2008. Goedert JJ, Rabkin CS, Ross SR. Prevalence of serologic reactivity against four strains of mouse mammary tumour virus among US women with breast cancer. Br J Cancer. 94:548-551, 2006. Golovkina TV, Dudley JP, Ross SR. Superantigen activity is need for mouse mammary tumor virus spread within the mammary gland. J Immunol. 161:2375-82, 1998. Golovkina TV, Jaffe AB, Ross SR. Coexpression of exogenous and endogenous mouse mammary tumor virus RNA in vivo results in viral recombination and broadens the virus host range. J Virol. 68:5019-5026, 1994. Golovkina TV, Piazzon I, Nepomnaschy I, Buggiano V, de Olano Vela M, Ross SR. Generation of a tumorigenic milk-borne mouse mammary tumor virus by recombination between endogenous and exogenous viruses. J Virol. 71:3895-3903, 1997. Grone A, Weckmann MT, Capen CC, Rosol TJ. Canine glyceraldehyde-3-phosphate dehydrogenase complementary DNA: polymerase chain reaction amplification, cloning, partial sequence analysis, and use as loading control in ribonuclease protection assays. Am J Vet Res. 57:254-257, 1996. Hachana M, Trimeche M, Ziadi S, Amara K, Gaddas N, Mokni M, Korbi S. Prevalence and characteristics of the MMTV-like associated breast carcinomas in Tunisia. Cancer Lett. 271:222-230, 2008. Hankinson SE, Colditz GA, Willett WC. Towards an integrated model for breast cancer etiology: the lifelong interplay of genes, lifestyle, and hormones. Breast Cancer Res. 6:213-218, 2004. Hayashiya S, Tani K, Morimoto M, Hayashi T, Hayasaki M, Nomura T, Une S, Nakaichi M, Taura Y. Expression of T helper 1 and T helper 2 cytokine mRNAs in freshly isolated peripheral blood mononuclear cells from dogs with atopic dermatitis. J Vet Med A Physiol Pathol Clin Med. 49:27-31, 2002. Held W, Waanders G, Shakhov AN, Scarpellino L, Acha-Orbea H, MacDonald HR. Superantigen-induced immune stimulation amplifies mouse mammary tumor virus infection and allows virus transmission. Cell 74:529-540, 1993. Holder WD and Wells SA. Antibody reacting with murine mammary tumour virus in the serum of patients with breast cancer: possible serological detection method for breast cancinoma. Cancer Res. 43:239-244, 1983. Holland JF and Pogo BG. Mouse mammary virus-like viral infection and human breast cancer. Clin Cancer Res. 10:5647-5649, 2004. Howard DK, Schlom J. Isolation of host-range variants of mouse mammary tumor viruses that efficiently infect cells in vitro. Proc Natl Acad Sci USA. 75:5718-5722, 1978. Howard DK, Schlom J. Isolation of a series of novel variants of murine mammary tumor viruses with broadened host ranges. Int J Cancer. 25:647-654, 1980. Hsu WL, Huang HM, Liao JW, Wong ML, Chang S-C. Increased survival in dogs with malignant mammary tumours overexpressing HER-2 protein and detection of a silent single nucleotide polymorphism in the canine HER-2 gene, Vet. J. 180: 116-123, 2009. Indik S, Gunzburg WH, Salmons B. Mouse mammary tumor virus infects human cells. Cancer Res. 65:6651-6659, 2005. Indik S, Gunzburg WH, Kulich P. Rapid spread of mouse mammary tumor virus in cultured human breast cells. Retrovirology. 4:73, 2007. Jacobs TW, Gown AM, Yaziji H, Barnes MJ, Schnitt SJ. Specificity of Hercep Test in determining HER-2/neu status of breast cancers using the United States Food and Drug Administration-approved scoring system. J Clin Oncol. 17:1983-1987, 1999. Jakobovits A, Shackleford GM, Varmus HE, Martin GR. Two proto-oncogenes implicated in mammary carcinogenesis, int-1 and int-2, are independently regulated during mouse development. Proc Natl Acad Sci USA. 83:7806-7810, 1986. Katz E, Lareef MH, Rassa JC, Grande SM, King LB, Russo J, Ross SR, Monroe JG. MMTV Env encodes an ITAM responsible for transformation of mammary epithelial cells in three-dimensional culture. J Exp Med. 201:431-439, 2005. Kozak C, Peters G, Pauley R,Morris V, Michalides R, Dudley J, GreenM, Davisson M, Prakash O, Vaidya A, et al. A standardized nomenclature for endogenous mouse mammary tumor viruses. J Virol. 61:1651-1654, 1987. Labrecque LG, Barnes DM, Fentiman IS, Griffin BE. Epstein-Barr virus in epithelial cell tumors: a breast cancer study. Cancer Res. 55:39-45, 1995. Laumbacher B, Fellerhoff B, Herzberger B, Wank R. Do dogs harbour risk factors for human breast cancer? Med Hypotheses 67:21-26, 2006. Lawson JS, Dinh D, Tran DD, Ford CE, Rawlinson WD. Elevated expression of the tumor suppressing protein p53 is associated with the presence of mouse mammary tumor-like env gene sequences (MMTV-like) in human breast cancer. Breast Cancer Res. Treat. 87:13-17, 2004. Lawson JS, Tran DD, Carpenter E, Ford CE, Rawlinson WD, Whitaker NJ, Delprado W. Presence of mouse mammary tumour-like virus gene sequences may be associated with morphology of specific human breast cancer. J Clin Pathol. 59:1287-1292, 2006. Levine PH, Pogo BG, Klouj A, Coronel S, Woodson K, Melana SM, Mourali N, Holland JF. Increasing evidence for a human breast carcinoma virus with geographic differences. Cancer. 101:721-726, 2004. Liu B, Wang Y, Melana SM, Pelisson I, Najfeld V, Holland JF, Pogo BG. Identification of a proviral structure in human breast cancer. Cancer Res. 61: 1754-1759, 2001. Luo T, Wu XT, Zhang MM, Qian K. Study of mouse mammary tumor virus-like gene sequences expressing in breast tumors of Chinese women. Sichuan Da Xue Xue Bao Yi Xue Ban. 37:844-846, 851, 2006. Mant C, Cason J. A human murine mammary tumour virus-like agent is an unconvincing etiological agent for human breast cancer. Cancer Res. 61:1754-1759, 2004. Mant C, Gillett CD, Arrigo C, Cason J. Human murine mammary tumor virus-like agents are genetically distinct from endogenous retroviruses and are not detectable in breast cell lines or biopsies. Virology. 318:393-403, 2004. Martin P, Ruiz SR, del Hoyo GM, Anjuere F, Vargas HH, Lopez-Bravo M, Ardavin C. Dramatic increase in lymph node dendritic cell numbers during infection by the mouse mammary tumor virus occurs by a CD62L-dependent blood-borne DC recruitment. Blood. 99:1282-8, 2002. Matsuzawa A, Nakano H, Yoshimoto T, Sayama K. Biology of mouse mammary tumor virus (MMTV). Cancer Lett. 90:3-11, 1995. Melana SM, Holland JF, Pogo BG. Search for mouse mammary tumor virus-like env sequences in cancer and normal breast from the same individuals, Clin. Cancer Res. 7:283-284, 2001. Melana SM, Picconi MA, Rossi C, Mural J, Alonio LV, Teyssie A, Holland JF, Pogo BG. Detection of murine mammary tumor virus (MMTV) env gene-like sequences in breast cancer from Argentine patients. Medicina (B Aires). 62:323-327, 2002. Melana SM, Nepomnaschy I, Sakalian M. Characterization of viral particles isolated from primary cultures of human breast cancer cells. Cancer Res. 67:8960-8965, 2007. Melana SM, Picconi MA, Rossi C, Mural J, Alonio LV, Teyssie A, Holland JF, Pogo BG. Detection of murine mammary tumor virus (MMTV) env gene-like sequences in breast cancer from Argentine patients, Medicina. (B Aires) 62:323-327, 2002. Michalides R, van Nie R, Nusse R, Hynes NE, Groner B. Mammary tumor virus induction loci in GR and DBAf mice contain one provirus of the mouse mammary tumor virus. Cell. 23:165-73, 1981. Michalides R, Wagenaar E, Hilkens J, Hilgers J, Groner B, Hynes NE. Acquisition of proviral DNA of mouse mammary tumor virus in thymic leukemia cells from GR mice. J Virol. 43:819-829, 1982. Misdorp, W, Else, RW, Hellme'n E, Lipscomb TP. Histological Classification of Mammary Tumors of the Dog and the Cat, 2nd series, Vol. VII, Armed Forces Institute of Pathology, American Registry of Pathology, Washington, DC and the World Health Organization Collaborating Center for Worldwide Reference on Comparative Oncology. 1-59, 1999. Mok MT, Lawson JS, Iacopetta BJ. Mouse mammary tumor virus-like env sequences in human breast cancer. Int J Cancer. 122:2864-2870, 2008. Nandi S, McGrath CM. Mammary neoplasia in mice. Adv Cancer Res.17:353-414, 1973. Nusse R. Insertional mutagenesis in mouse mammary tumorigenesis. Curr Top Microbiol Immunol. 171:43-65,1991. Nusse R, van Ooyen A, Rijsewijk F, van Lohuizen M, Schuuring E, van't Veer L. Retroviral insertional mutagenesis in murine mammary cancer. Proc R Soc Lond B Biol Sci. 226:3-13, 1985. O'Neill LAJ. The interleukin-1 receptor/Toll-like receptor superfamily: signal transduction during inflammation and host defense. Sci STKE. 44:1-11, 2000. Ono M, Yasunaga T, Miyata T, Ushikubo H: Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome. J Virol. 60:589-598, 1986. Pogo BG, Melana SM, Holland JF, Mandeli JF, Pilotti S, Casalini P, Menard S. Sequences homologous to the mouse mammary tumor virus env gene in human breast carcinoma correlate with overexpression of laminin receptor. Clin. Cancer Res. 5:2108-2111, 1999. Rassa JC, Meyers JL, Zhang Y, Kudaravalli R, Ross SR. Murine retroviruses activate B cells via interaction with Toll-like receptor 4. Proc Natl Acad Sci U S A. 99: 2281-2286, 2002. Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologe. 8:138-140, 1987. Ross SR. Using genetics to probe host-virus interactions: the mouse mammary tumor virus model. Microbes Infect. 2:1215-23, 2000. Ross SR. MMTV Infectious Cycle and the Contribution of Virus-encoded Proteins to Transformation of Mammary Tissue. J Mammary Gland Biol Neoplasia. 13:299-307, 2008. Ross SR, Schmidt JW, Katz E, Cappelli L, Hultine S, Gimotty P, Monroe JG. An immunoreceptor tyrosine activation motif in the Mouse Mammary Tumor Virus envelope protein plays a role in virus induced mammary tumors. J Virol. 80:9000-9008, 2006. Segev N, Hizi A, Kirenberg F and Keydar I. Characterization of a protein, released by T47D cell-line, immunologically related to the major envelope protein of the mouse mammary tumour virus. Proc Nat Acad Sci USA. 82:1531-1535, 1985. Shackleford GM, Varmus HE. Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos. Cell. 50:89-95, 1987. Shackleford GM, MacArthur CA, Kwan HC, Varmus HE. Mouse mammary tumor virus infection accelerates mammary carcinogenesis in Wnt-1 transgenic mice by insertional activation of int-2/Fgf-3 and hst/Fgf-4. Proc Natl Acad Sci USA. 90:740-744, 1993. Stewart TH, Sage RD, Stewart AF, Cameron DW. Breast cancer incidence highest in the range of one species of house mouse, Mus domesticus. Br J Cancer. 82:446-451, 2000. Szabo S, Haislip AM, Garry RF. Of mice, cats, and men: is human breast cancer a zoonosis? Microsc Res Tech. 68:197-208, 2005a. Szabo S, Haislip AM, Traina-Dorge V, Costin JM, Crawford BE, II, Wilson RB, Garry RF. Human, rhesus macaque, and feline sequences highly similar to mouse mammary tumor virus sequences. Microsc Res Tech. 68:209-221, 2005b. Szabo CI and King MC. Population genetics of BRCA1 and BRCA2. Am J Hum Genet. 60:1013-1020, 1997. Vacheron S, Luther SJ, Acha-Orbea H. Preferential infection of immature dendritic cells and B cells by mouse mammary tumor virus. J Immunol. 168:3470-3476, 2002. Velin D, Fotopopulos G, Luthi F, Kraehenbuhl JP. The nasalassociated lymphoid tissue of adult mice acts as an entry site for the mouse mammary tumor retrovirus. J Exp Med. 185:871-876, 1997. Wang Y, Go V, Holland JF, Melana SM, Pogo BG. Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer. Clin Cancer Res. 4:2565-2568, 1998. Wang Y, Holland JF, Bleiweiss IJ, Melana S, Liu X, Pelisson I, Cantarella A, Stellrecht K, Mani S, Pogo BG. Detection of mammary tumor virus env gene-like sequences in human breast cancer. Cancer Res. 55:5173-5179, 1995. Wang Y, Jiang JD, Xu D. A mouse mammary tumor viruslike long terminal repeat superantigen in human breast cancer. Cancer Res. 64:4105-4111, 2004. Wang Y, Melana SM, Baker B, Bleiweiss I, Fernandez-Cobo M, Mandeli JF, Holland JF, Pogo BG. High prevalence of MMTV-like env gene sequences in gestational breast cancer. Med Oncol. 20:233-236, 2003. Wang Y, Pelisson I, Melana SM, Go V, Holland JF, Pogo BG. MMTV-like env gene sequences in human cancer. Arch Virol. 146: 171-180, 2001. Witt A, Hartmann B, Marton E, Schreiber M, Kubista. The mouse mammary tumor virus-like env gene sequence is not detectable in breast cancer tissue of Austrian patients. Oncology Rep. 10:1025-102, 2003. Yanagawa S, Kakimi K, Tanaka H, Murakami A, Nakagawa Y, Kubo Y, Yamada Y, Hiai H, Kuribayashi K, Masuda T. Mouse mammary tumor virus with rearranged long terminal repeats causes murine lymphomas. J Virol. 67:112-118, 1993. Yanagawa S, Lee JS, Kakimi K, Matsuda Y, Honjo T, Ishimoto A. Identification of Notch1 as a frequent target for provirus insertional mutagenesis in T-cell lymphomas induced by leukemogenic mutants of mouse mammary tumor virus. J Virol. 74: 9786-9791, 2000. Zammarchi F, Pistello M, Piersigilli A, Murr R, Di Cristofano C, Naccarato AG, Bevilacqua G. MMTV-like sequences in human breast cancer: a fluorescent PCR/laser microdissection approach. J Pathol. 209:436-444, 2006. Zangen R, Harden S, Cohen D, Parrella P, Sidransky D.Mouse mammary tumor-like env gene as a molecular marker for breast cancer? Int J. Cancer. 102:304-307, 2002. Zapata-Benavides P, Saavedra-Alonso S, Zamora-Avila D, Vargas-Rodarte C, Barrera-Rodriguez R, Salinas-Silva J, Rodriguez-Padilla C, Tamez-Guerra R, Trejo-Avila L. Mouse mammary tumor virus-like gene sequences in breast cancer samples of Mexican women. Intervirology. 50:402-407, 2007.
摘要: 目前推測許多環境因子皆可能為乳癌的形成原因,其中包括老鼠乳腺腫瘤病毒(mouse mammary tumor virus, MMTV)。老鼠乳腺腫瘤病毒為致癌B型反轉錄病毒,傳染途徑分為內源性及外源性感染,皆能誘導小鼠乳癌之形成。已有許多研究報告發現人類乳癌中高度表現類似老鼠乳腺腫瘤之病毒序列,由此顯示老鼠乳腺腫瘤病毒與人類乳癌的關係。遂有假說推斷因犬貓經常與人類接觸,而從老鼠媒介MMTV或MMTV-like virus傳播至人類。但目前尚無實驗室研究犬貓之乳癌組織中是否有老鼠乳腺腫瘤病毒或類似老鼠乳腺腫瘤病毒之序列。由於此假說仍未有進一步的研究證實,遂本實驗之研究目的為使用nested PCR方法,偵測犬貓乳腺腫瘤組織是否有類似老鼠乳腺腫瘤病毒之序列。若能從組織中偵測到類似序列,將會與老鼠乳腺腫瘤病毒比對序列相似性。另外同時檢視臨床與病理因子,進行多重分析,觀察結果是否與序列的出現有相關性。 實驗樣本的取得是來自中興大學獸醫教學醫院 (於西元1995年至2008年) 經由外科手術切除之乳腺腫瘤組織,來自113隻狗及11隻貓,結果發現在MMTV-like env and LTR gene sequences在犬惡性乳癌組織中的出現比例分別為3.49%及18.6%,而在貓惡性乳腺腫瘤組織中的出現比例分別為22.2%及22.2%;DNA定序結果,與陽性對照組及MMTV的基因相似性有94–98 %,且在犬隻偵測的MMTV-like LTR gene sequences與MMTV的基因相似性較低 (大約89%)。另外,可以在犬貓正常乳腺組織中偵測出序列之動物,其鄰近之乳腺腫瘤也同樣偵測出序列,遂推測此序列或許為內源性來源。我們無法排除此序列為來自老鼠之可能性,目前也仍不清楚犬貓從老鼠媒介MMTV或MMTV-like virus之傳染途徑,因此仍須進一步證實犬貓乳腺腫瘤組織中是否出現老鼠乳腺腫瘤病毒顆粒。
Mouse mammary tumor virus (MMTV), an oncogenic B retrovirus, contributes to occurrence of breast cancer in mice, acquired horizontally through the milk (exogenous) to susceptible offspring or vertically through the germ line (endogenous provirus). The MMTV-like sequences have been found in human breast cancer, implicating the involvement of MMTV in human breast cancer. Dogs and cats with frequent human contacts were proposed to contribute to transmission of this virus between mouse and man; however, this hypothesis has not yet been defined. Hence, the goal of this study was to detect MMTV-like gene sequences in canine and feline mammary tumors. MMTV-like env and LTR sequence were amplified by means of nested PCR. The sequences of resultant DNA fragments were verified by direct automated sequencing. Moreover, we also examine clinical and pathological parameters by multivariate analysis, and find the association with the presence of the MMTV-like sequences. In total of 124 samples from 113 dogs and 11 cats who had undergone surgery were obtained from 1995 to 2008 in Veterinary Medical Teaching Hospital, National Chung Hsing University. Specific MMTV-like env and LTR sequences were found in 3 (3.49%) and 16 (18.6%) of dogs with malignant mammary tumors, respectively. Besides this, specific MMTV-like env and LTR sequences were also detected in 2 (22.2%) and 2 (22.2%) of feline malignant mammary tumors, respectively. The homology of these sequences to MMTV identified from NIH 3T3 (MMTV-positive murine cell line) and MMTV-like sequences approximately range from 94% to 98%; however, a lower similarity (approximately 89%) was observed when MMTV-like LTR sequences from canine were aligned with those of MMTV identified from mouse (HeJ strain). Moreover, sequences of LTR present in 2 dogs (13.3%, 2/15) and sequences of LTR present in one cat (50.0%, 1/2), both in mammary tumor and in mammary normal tissue suggesting that it may be an endogenous origin. Nevertheless, it can't be ruled out completely the possibility of the transmission from mice. The role of dog and cat plays in transmission of MMTV from mice to human remains unclear. In this respect, isolation of MMTV particles from such animals would be crucial.
其他識別: U0005-3006200901503300
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