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Serial changes of the polymorphic cell in Actinobacillus pleuropneumonia
|摘要:||中 文 摘 要
Abstract The polymorphic cells, arranged in swirling pattern, are usually in the pneumonic lesion of Actinobacillus pleuropneumoniae (App)-infected pig. Specific pathogen free pigs were inoculated intratracheally with App. at a concentration of 5×105 CFU, in order to understand the origin and formation processes of polymorphic cells. The pigs either died spontaneously, or were sacrificed at 12th and 24th hour after inoculation. The cells in alveolar spaces were observed with light and electron microscopes. The results indicated that in the early stage after App. inoculation, a lot of neutrophils were observed in alveolar spaces. Twenty-four hours later, polymorphic cell appeared abundantly. Although most cells were necrotic and their organelles disappeared, some cells still could be identified. Ultrastructural findings indicated that some were monocytes and macrophages, in which lysosomes were observed, and others were type Ⅱ pneumocytes, in which, lamellar bodies were observed. Two formation processes of polymorphic cells were observed. One was that monocytes migrated to alveolar spaces after endothelial cells were damaged or ruptured. The other was that alveolar macrophages and type Ⅱ pneumocytes gradually deformed after injured by App. The mechanisms of deformation were nuclear envelope blebbing, mitochondria swelling, rough endoplasmic reticulum dilatation, and cytoplasmic swelling to squeeze the nucleus. Conclusively, the appearance of polymorphic cells was relatively specific lesion of Actinobacillus pleuropneumonia. It was verified that these cells were derived from monocytes, macrophages and type Ⅱ pneumocytes.
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