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標題: 台灣中部地區乳羊繁殖季節期間糞孕酮濃度之變化
Fecal progesterone profile during the breeding season of dairy goats in central Taiwan
作者: 介美莉
Jack, Amelia MM
關鍵字: dairy goats
fecal progesterone
出版社: 獸醫學系暨研究所
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摘要: 糞便荷爾蒙分析為一種以非侵襲性的方式來達到監測動物繁殖內分泌的新方法,主要用來分析雌性動物性類固醇荷爾蒙的變化,以瞭解其動情週期,懷孕狀態或繁殖障礙等問題,因採樣操作簡單,近年來逐漸受到廣泛的應用。本研究以糞孕酮濃度之分析來進行乳羊的早期懷孕診斷、建立動情週期及懷孕期孕酮濃度變化的輪廓、以及預測乳羊分娩日期及其胎仔數。本實驗從2008年十月至2010年四月於中興大學的乳羊場進行試驗,共對17隻自然交配的母山羊定期進行糞便採樣,採樣時間由交配第一天開始固定每周採樣3次,共22周,利用酵素結合免疫吸附分析方法來檢測其孕酮濃度,並於配種後2個月以腹部超音波掃描來確診羊隻的懷孕。實驗結果顯示,懷孕與未懷孕羊隻於交配後19-20天的平均糞孕酮濃度達顯著性的差異 (2492.4 ± 69.0 vs 577.8 ± 82.0 ng/g,p < 0.05),且其敏感度、特異性及正確度皆達100%。以孕酮濃度檢測母羊平均動情週期長度為20.8 ± 0.6天。孕酮濃度的基礎值為344.3 ng/g,而黃體期孕酮濃度於週期第9-13天達最高,且於下個動情期來臨的前4天呈現顯著性的下降 (p < 0.05)。母羊懷孕期平均為149.3 ± 0.2天,其每周平均孕酮濃度變化顯示於配種後第7周開始慢慢上升,至第14周達最高,且維持高值至第21周,於分娩前6天急遽下降,並於分娩前2天下降幅度呈現顯著性的差異 (3884.3 vs 1205.0 ng/g,p < 0.05)。本實驗並未觀察到母羊懷孕期與其胎仔數、產次、品種或交配月份的相關性。平均產仔數為2.1胎,且孕酮濃度高低與產仔數的多寡只有呈現非顯著性的相關趨勢。根據本實驗之結果可知,以山羊糞孕酮濃度的分析可作為監控其繁殖狀態的新方法。
There is an increasing interest in the application of fecal hormone analysis as an alternative approach to non-invasive reproductive assessment, primarily owing to the relative ease of sample collection from animals. Fecal steroid assays have mainly been used to study female reproduction and provide information regarding the estrus cycle, pregnancy and reproductive disorders. The present study was undertaken to evaluate the use of fecal progesterone (FP4) concentration for early pregnancy diagnosis, mapping the FP4 profile during the estrous cycle and gestation period, in predicting the parturition date and the litter size in dairy goats. This experiment was realized on the dairy goat farm at Chung Hsing University between October 2008 and April 2010. Fecal samples were collected from 17 hand-mated goats 3 times weekly for 22 - 23 weeks, beginning on the day of mating. The levels of FP4 were determined by enzyme linked immunosorbent assay (ELISA). Pregnancy diagnosis was performed at 2 months post-mating by trans-abdominal ultrasonography and confirmed upon birth of offsprings. The results indicated a significant difference in the mean FP4 concentration obtained during days 19 - 20 post-mating between pregnant and non-pregnant does (2492.4 ± 69.0 vs 577.8 ± 82.0 ng/g, p < 0.05). A sensitivity, specificity and accuracy of 100% each was obtained applying FP4 concentration analysis on days 19 - 20 post-mating for pregnancy diagnosis. Mean estrous cycle length was 20.8 ± 0.6 days. A baseline FP4 value of 344.3 ng/g was obtained. The highest FP4 concentrations were recorded during days 9 to 13 of the luteal phase. A significant drop in the FP4 concentration was noted 4 days before the estimated day of estrus (p < 0.05). A gestation length averaging 149.3 ± 0.2 days was observed. The mean weekly FP4 profile obtained in this study showed a progressive increase from week 7 to 14 until a plateau was reached between weeks 15 and 21, and then a rapid decline beginning 6 days before with a significant drop (p < 0.05) 2 days (from 3884.3 to 1205.0 ng/g) pre-partum. In the present study, a correlation between gestation length and litter size, parity, breed or month of mating was not observed. Average litter size was 2.1 kids. No significant correlation was observed between FP4 concentration and the number of kids born. In conclusion, the measurement of FP4 concentration is a potentially alternative method for monitoring reproductive status in goats.
其他識別: U0005-2110201011074500
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