Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/14351
標題: 以冷光化學免疫試驗偵測各種腫瘤患犬之α型胎兒蛋白受體血清量
Serum level of alpha-fetoprotein receptor using chemiluminescence immunoassay in dog with various tumor types
作者: 莊正瑋
Chuang, Cheng-Wei
關鍵字: α型胎兒蛋白受體
alpha-fetoprotein
α型胎兒蛋白
腫瘤偵測
alpha-fetoprotein receptor
tumor detection
出版社: 獸醫學系暨研究所
引用: Aagaard-Tillery, K.M., Buchbinder, A., Boente, M.P., Ramin, K.D., 2007. Beckwith-Wiedemann syndrome presenting with an elevated triple screen in the second trimester of pregnancy. Fetal Diagn Ther 22, 18-22. Abelev, G.I., Elgort, D.A., 1982. Alpha-fetoprotein, in Cancer. Medicine Holland J, Frei E, eds II Edition,. Lea and Febiger, New York Alpert E. Human alpha-1 fetoprotein. In: Okuda K, Peters RL, eds. Hepatocellular carcinoma. New York: John Wiley & Sons, 1976. 353-367. Abelev, G.I., Eraiser, T.L., 1999. Cellular aspects of alpha-fetoprotein reexpression in tumors. Semin Cancer Biol 9, 95-107. Abelev, G.I., Perova, S.D., Khramkova N.I., Postnikova E.A., Irlin I.S., 1963. Production of embryonal alpha-globulin by transplantable mouse hepatoma. Transplantation 1, 174–178. Alexander, P., 1972. Fetal antigens in cancer. Nature 235, 137–140. Ball, D., Rose, E., Alpert, E., 1990. Biological variation of alpha-fetoprotein levels in normal adults I Abstract. Gastroenterology 9, 271. Ball, D., Rose, E., Alpert, E., 1992. Alpha-fetoprotein levels in normal adults. Am J Med Sci 303:157-159. Baker, M.E., 1988. Evolution of alpha-fetoprotein: sequence comparisons among AFP species and with albumin species. Tumour Biol 9, 123-36. Bakker, J., De Mees, C., Douhard, Q., Balthazart, J., Gabant, P., Szpirer, J., Szpirer, C., 2006. Alpha-fetoprotein protects the developing female mouse brain from masculinization and defeminization by estrogens. Nat Neurosci 9, 220–226. Baschat, A.A., Harman, C.R., Farid, G., Chodirker, B.N., Evans J.A., 2002. Very low second-trimester maternal serum alpha-fetoprotein: association with high birth weight. Obstet Gynecol 99, 531–536. Benno, R.H., Williams, T.H., 1978. Evidence for intracellular localization of AFP in the developing rat brain. Brain Res. 142–146. Bergstrand, C.G., 1986. Alphafetoprotein in paediatrics. Acta Paediatr Scand 75:1– 9. Bergstrand ,C.G., Czar, B., 1957. Paper electrophoretic study of human fetal serum proteins with demonstration of a new protein fraction. Scand J Clin Lab Invest 9, 277–281 Blair, J.I., Carachi, R., Gupta, R., Sim, F.G., McAllister, E.J., Weston, R., 1987. Plasma alpha fetoprotein reference ranges in infancy: effect of prematurity. Arch Dis Child 62, 362 –369. Brock, D.J.H., Sutcliffe, R.G., 1972. Alpha-fetoprotein in the antenatal diagnosis of anencephaly and spina bifida. Lancet 2, 191–194. Brumm, C., Schulze, C., Charels, K., Morohoshi, T., Kloppel, G., 1989. The significance of alpha-fetoprotein and other tumor markers in differential immunocytochemistry of primary liver tumors. Histopathology 14, 503-513. Caballero, C., Vekemans, M., Lopez del Campo, J.G., Robyn, C., 1977. Serum alpha-fetoprotein in adults, in women during pregnancy, in children at birth, and during the first week of life: a sex difference. Am J Obstet Gynecol 127, 384–389. Chandra, R.K., Madhavankutty, K., Way, R.C., 1975. Serum alpha-fetoprotein levels in patients with cystic fibrosis and their parents and siblings. Br Med J 29,714-716. Cho, S., Durfee, K.K., Keel, B.A., Parks, L.H., 1997. Perinatal outcomes in a prospective matched pair study of pregnancy and unexplained elevated or low AFP screening. J Perinat Med 25, 476-483. Christiansen, M., Andersen, J.R., Terning, J., Overg~rd, O., Jensen, S.P., Magid, E., Negaard-Pedersen B., 1994. Serum alpha-fetoprotein and alcohol consumption. Scand J Clin Lab Invest 54, 215-220. Chung, W.C., Pak, T.R., Weiser, M.J., Hinds, L.R., Andersen, M.E., Handa, R.J., 2006 Progestin receptor expression in the developing rat brain depends upon activation of estrogen receptor alpha and not estrogen receptor beta. Brain Res 1082, 50–60. Crandall, B.F., 1981. Alpha-fetoprotein: a review. CRC Crit Rev Clin Lab Sci 15, 127-185. Crandall, B.F., Chua, C., 1997. Risks for fetal abnormalities after very and moderately elevated AF-AFPs. Prenat Diagn 17, 837–841. Cuckle, H., 2000. Biochemical screening for Down syndrome. Eur J Obstet Gynecol Reprod Biol 92, 97–101 Deutsch, H.F., 1991. Chemistry and biology of a-fetoprotein. Adv Cancer Res 56, 253-312. Dziadek M.A., Andrews G.K., 1983. Tissue specificity of alpha-fetoprotein messenger RNA expression during mouse embryogenesis. EMBO J 2, 549-554. Entezami, M., Runkel, S., Sarioglu, N., Hese, S., Weitzel, H.K., 1997. Diagnostic dilemma with elevated level of alpha-fetoprotein in an undiagnosed twin pregnancy with a small discordant holoacardius acephalus. Am J Obstet Gynecol 177, 466–468. Fryer, A.A,, Jones, P., Strange, R., Hume, R., Bell, J.E., 1993. Plasma protein levels in normal human fetuses: 13 to 41 weeks’ gestation. British journal of obstetrics and gynaecology 100, 850- 855. Geuskens, M., Naval, J., Uriel, J., 1986. Ultra-structural studies of the intracellular translocation of endocytosed alpha-fetoprotein (AFP) by cytochemistry and of the uptake of H-arachidonic acid bound to AFP by autoradiography in rat rhabdomyosarcoma cells. J Cell Physiol 128, 389–396. Gillespie, J.R., Uversky, V.N., 2000. Structure and function of alpha-fetoprotein: a biophysical overview. Biochim Biophys Acta 1480, 41-56. Gitlin, D., 1975. Normal biology of α-fetoprotein. Annals of the New York Academy of Sciences 259, 7-16. Gleiberman, A.S., Abelev, G.I., 1985. Cell position and cell interactions in expression of fetal phenotype of hepatocyte. Int Rev Cytol 95, 229-266. Hajeri-Germond, M., Trojan, J., Uriel, J., Hauw, J.J., 1983. In vitro uptake of exogenous AFP by chicken dorsal root ganglia. Dev Neurosci 6, 11–15. Hida, S., Nishimura, K., Nishio, Y., Oishi, K., Takeuchi, H., Yoshida, O., 1991. VAB-6 chemotherapy causes spurious elevation of alpha-fetoprotein associated with liver dysfunctions. Urol Int 46, 1-5. Hirai, H., 1979. Model systems of AFP and CEA expression. Methods Cancer Res 18, 39-97. Kanevsky, VY., Pozdnyakova, L.P., Aksenova, O.A., Severin, S.E., Karakov, V.Y., Severin E.S., 1997. Isolation and characterization of AFP binding proteins from tumor and fetal tissues. Biochem Mol Biol Internl 41, 1143–1151. Kao, W.Y., Chiou, Y.Y., Hung, H.H., Su, C.W., Chou, Y.H., Wu, J.C., Huo, T.I., Huang Y.H., Wu W.C., Lin H.C., Lee S.D., 2012. Serum alpha-fetoprotein response can predict prognosis in hepatocellular carcinoma patients undergoing radiofrequency ablation therapy. Clin Radiol 67, 429-436. Kiran, T.S., Bethel, J., Bhal, P.S., 2005. Correlation of abnormal second trimester maternal serum alpha-fetoprotein (MSAFP) levels and adverse pregnancy outcome. J Obstet Gynaecol 25, 253–256. Kirkitadze, M.D., Narizhieva, N.V., Tomashevskiĭ, A.Iu., Potekhin, S.A., Uverskiĭ, V.N., 1996. Stabilization of alpha-fetoprotein structure by sucrose, Bioorgan. Chem 22, 408-414. Kondo, F., Wada, K., Nagato, Y., Nakajima, T., Kondo, Y., Hirooka, N., Ebara, M., Ohto, M., Okuda, K., 1989. Biopsy diagnosis of well-differentiated hepatocellular carcinoma based on new morphologic criteria. Hepatology. 9, 751-755. Laderoute, M.P., 1991. The characterization of a novel, widespread, PNA-reactive tumor associated antigen: The alphafetoprotein receptor/binding protein. Ph.D. Thesis, Faculty of Graduate Studies and Research, University of Alberta, Canada, pp 1–256. Laderoute, M.P., Wilans, D., Wegmann, T., Longenecker, M., 1994. The identification, isolation, and characterization of a 67-kilodalton, PNA-reactive autoantigen commonly expressed in human adenocarcinomas. Anticancer Res. 14, 1233–1246. Lambert, J.C., Seralini, G.E., Stora, C., Vallette, G., Vranckx, R., Nunez, E.A., 1986. Effects of alphafetoprotein on isolated mouse oocytes. Tumour Biol 7, 91–97. Lechowski, R., Jagielski, D., Hoffmann-Jagielska, M., Zmudzka, M., Winnicka, A., 2002. Alpha-fetoprotein in canine multicentric lymphoma. Vet Res Commun 26, 285-296. Lehmann, F-G., Wegener, T., 1979. Alpha-fetoprotein in liver cirrhosis. I. Prognostic and diagnostic significance of the transient rise of serum alpha-fetoprotein. In: Lehmann F-G, ed. Carcinoembryonic proteins. Vol I. Amsterdam:Elsevier/ North-Holland Biomedical Press, 219-231. Lei, U., Wohlfahrt, J., Christens, P., Westergaard, T., Lambe, M., Norgaard-Pedersen, B., Melbye, M., 2004. Reproductive factors and extreme levels of maternal serum alpha-fetoprotein: a population-based study. Acta Obstet Gynecol Scand 83,1147-1151. Li, M., Zhu, M., Li, W., Lu, Y., Xie, X., Wu, Y., Zheng, S., 2013. Alpha-fetoprotein receptor as an early indicator of HBx-driven hepatocarcinogenesis and its applications in tracing cancer cell metastasis. Cancer Lett 330, 170-180. Lowseth, L.A., Gillett, N.A., Chang, I-Y., Muggenburg, B.A., Boecker B.B., 1991. Detection of serum α-fetoprotein in dogs with hepatic tumors. J. Am. Vet. Med. Assoc 199, 735–741. Madsen, A.C., Rikkers, L.F., Moody, F.R. Moody, F.G., Wu, J.T., 1980. α-Fetoprotein as a marker for hepatic regeneration in the dogs. J. Surg. Res 28, 71–76. Mizejewski, G.J, 1985. New insights into AFP structure and function: potential biomedical applications. In: Mizejewski GJ, Porter IH, Eds. Alpha-fetoprotein and Congenital Disorders. Orlando: Academic Press, pp5–34. Mizejewski, G.J, 1995. The phylogeny of alpha-fetoprotein in vertebrates:survey of biochemical and physiological data. Crit Rev Eukaryot Gene Expr 5, 281–316. Mizejewski, G.J, 1997. Alpha-fetoprotein as a biologic response modifier: relevance to domain and subdomain structure. Proc Soc Exp Biol 215, 333–362.. Mizejewski, G.J., 2003.Levels of alpha-fetoprotein during pregnancy and early infancy in normal and disease states. Obstet Gynecol Surv 58, 804–826. Mizejewski, G.J., 2007. Physiology of alpha-fetoprotein as a biomarker for perinatal distress: relevance to adverse pregnancy outcome. Exp Biol 8, 993-1004. Mizjewswki, G.J., 2009. Mapping on structure-function peptide sites on the human alpha-fetoprotein amino acid sequence. Atlas of Genetics and Cytogenetics in Oncology and Haematology. Deep Insight Section, 1–65.254 Tumor Biol. (2011) 32:241–258, 2009. Mizejewski, G.J, 2011. Review of the putative cell-surface receptors for alpha-fetoprotein: identification of a candidate receptor protein family. Tumour Biol 32, 241-258. Moawad, A.H., Goldenberg, R.L., Mercer, B., Meis, P.J., Iams, J.D., Das A., Caritis, S.N., Miodovnik M., Menard M.K., Thurnau G.R., Dombrowski M., Roberts J.M.; NICHD MFMU Network, 2002. The Preterm Prediction Study: the value of serum alkaline phosphatase, alpha-fetoprotein, plasma corticotropin-releasing hormone, and other serum markers for the prediction of spontaneous preterm birth. Am J Obstet Gynecol 186, 990-996. Morinaga, T., Sakai, M., Wegmann, T.G., Tamaoki, T., 1983. Primary structures of human alpha-fetoprotein and its mRNA. Proc Natl Acad Sci 80, 4604-4608. Moro, R., Uriel, J., 1981. Early localization of AFP in the developing nervous system of the chicken. Onco-develop. Biol Med 2, 391–399. Moro, R., Gulyaeva-Tcherkassova, J., Stieber, P., 2012. Increased alpha-fetoprotein receptor in the serum of patients with early-stage breast cancer. Curr Oncol 19, e1-8. Muller, F., Dreux, S., Sault, C., Galland, A., Puissant, H., Couplet, G., Lemay, C., Larcher, M.E., Renom, G., 2003. Very low alpha-fetoprotein in Down syndrome maternal serum screening. Prenat Diagn 23, 584–587. Murakami, Y., Ohhigashi, S., Kohmo, N., Nakai, S., Kado, S., Masuda, T., Kohama, Y, et al. 1985. A case of a-fetoprotein (AFP)-producing gastric cancer: the review of 114 cases of AFP-producing gastric cancer in Japan. J Hiroshima Med Assoc 38, 1204-1212. Nishi, S., Hirai, H., 1972. Purification of human, dog and rabbit α-fetoprotein by immunoadsorbents of Sepharose coupled with anti-human α-fetoprotein. Biochemi. Biophys. Acta 278, 293–298. Naidu, S., Peterson, M.L., Spear, B.T., 2009. Alpha-fetoprotein related gene (ARG): a new member of the albumin gene family that is no longer functional in primates. Gene 449, 95–102. Nishi, S., Watabe, H., Hirai, H., 1973. Species crossreaction of α-fetoprotein and break-down of the tolerance toα-fetoprotein by immunization with heterologous α-fetoprotein.Tumor Res 8, 17–22. Nomura, F., Ohnishi, K., Tanabe, Y., 1989. Clinical features and prognosis of hepatocellular carcinoma with reference to serum alpha-fetoprotein levels. Cancer 64, 1700- 1707. Obiekwe, B.C., Malek, N., Kitau, M.J., Chard, T., 1985. Maternal and fetal alphafetoprotein (AFP) levels at term. Relation to sex, weight and gestation of the infant. Acta Obstet Gynecol Scand 64, 251-253. Odibo, A.O., Sehdev, H.M., Stamilio, D.M., Macones, G.A., 2006. Evaluating the thresholds of abnormal second trimester multiple marker screening tests associated with intra-uterine growth restriction. Am J Perinatol 23, 363–367. Okimoto, Y., Fujihara, T., ''I''suda, M., Kinoshita, M., Takaoka, M., Uchida,Y., Fukuda, Y., Imura H., 1989. An extremely rare adult case with high alpha-fetoprotein in serum and with normal liver cells stained partially with alpha-fetoprotein. Nihon Shokakibyo Gakkai Zasshi. 86, 2812-2815. Pecchio, F., Rapellino, M., Torre, G.C., 1989. International workshop on alpha-fetoprotein: new trends and perspectives. J Nucl Med Allied Sci 33 1-137. Pham, T.T., Benirschke, K., Masliah, E., Stocker, J.T., Yi, E.S., 2004. Congenital pulmonary airway malformation (congenital cystic adenomatoid malformation) with multiple extrapulmonary anomalies: autopsy report of a fetus at 19 weeks of gestation. Pediatr Dev Pathol 7, 661–666. Puts, D.A., Jordan, C.L., Breedlove, S.M., 2006. Defending the brain from estrogen. Nat Neurosci 9, 155–156. Rausch, D.N., Lambert-Messerlian, G.M., Canick, J.A., 2000. Participation in maternal serum screening for Down syndrome, neural tube defects, and trisomy 18 following screen-positive results in a previous pregnancy. West J Med 173, 180–183. Ruoslahti, E., Hiria, H., 1978. Alpha-fetoprotein. In: Norgaard-Pedersen B, Axelsen NH, editors. Cacinombryonic proteins: recent progress. Blackwell Scientific Publications, pp. 3e36. Ruoslahti, E., Salaspuro, M., Pihko, H., Anderson, L., Seppala, M., 1974. Serum alpha-fetoprotein: diagnostic significance in liver disease. Br Med J 8, 527-529. Seralini, G.E., Lafaurie, M., Krebs, B., Stora, C., 1986. Alphafetoprotein and atretic follicles in the ovary of the pregnant rat. Tumour Biol 7, 1–8. Seralini, G.E., Stora, C., 1987.Inhibition of murine genital function by alphafetoprotein. Tumour Biol 8, 292–293 Shenhav, S., Gemer, O., Volodarsky, M., Zohav, E., Segal, S., 2003. Midtrimester triple test levels in women with severe preeclampsia and HELLP syndrome. Acta Obstet Gynecol Scand 82, 912–915. Silver, H.K.B., Deneault, J., Gold, P., Thompson, W.G., Shuster, J., Freedman, S.O., 1974. The detection of cr,-fetoprotein in patients with viral hepatitis. Cancer Res 34, 244-247. Sritippayawan, S., Vachirasrisoontra, C., 2005. Adverse pregnancy outcomes after a false-positive second trimester serum screen for Down syndrome in Thai pregnant women. J Med Assoc Thai 88, 449–454. Sugmoto, M., Itsukaichi, T., lshii, K., Sumino, Y., Sugano, S., Abei, T., Imamura, Y., 1987. Marked increase in serum a-fetoprotein level in cirrhosis: a case report. Am J Gastroenterol 82,69-71. Suzuki, Y., Zeng, C.Q.Y., Alpert, E., 1992. Isolation and characterization of a specific alpha-fetoprotein receptor on human monocytes. J Clin Invest 90, 1530-1536. Taketa, K., 1990. Alpha-fetoprotein: reevaluation in hepatology. Hepatology 12, 1420-1432. Taketa, K., Watanabe, A., Kosaka, K., 1975.Different mechanisms of increased alpha-fetoprotein production in rats following CCI, intoxication and partial hepatectomy. Ann NY Acad Sci 259, 80-84. Tatarinov, Y.S., 1965. Content of embryo-specific alpha-globulin in the blood serum of the human fetus, newborn, and adult man in primary cancer of the liver. Vop Khim SSR 11, 20–24. Tcherkassova, J., Abramovich, C., Moro, R., Chen, C., Schmit, R., Gerber, A., Moro, R., 2011. Combination of CA125 and RECAF biomarkers for early detection of ovarian cancer. Tumour Biol 32, 831-838. Torres, J.M., Geuskens, M., Uriel, J., 1991. Receptor-mediated endocytosis and recycling of alpha-fetoprotein in human β-lymphoma and T-leukemia cells. Int J Cancer 47, 110–117. Trojan, J., Uriel, J., 1982. Immunocytochemical localization of AFP and serum albumin in ecto-, meso-, and endodermal tissue derivatives of the developing rat. Oncodevelop Biol Med 3,13–22. Tsuchida, Y., Endo, Y., Saito, S., Kaneko, M., Shiraki, K., Ohmi, K., 1978. Evaluation of alpha-fetoprotein in early infancy. J Pediatr Surg 13,155-156. Tsuboi. S., Taketa, K., Nouso, K., Fujikawa, T., Manabe, K., Ohmori, H., Higashi, T., 2006. Shiratori, Y., High level of expression of alpha-fetoprotein receptor in gastric cancers. Tumour Biol 27, 283-288. Ehrhart, E.J., Powers, B.E., 2007. The Pathology of Neoplasia. In: Withrow, S.J., Vail, D.M., Ed. Withrow and MacEwen’s Small Animal Clinical Oncology, Fourth Ed. Saunders Elsevier, St. Louis, MO, USA, pp. 503-510. Um, SH, Mulhall, C., Alisa, A., Ives, A.R., Karani, J., Williams, R., Bertoletti, A., Behboudi, S.,2004. Alpha-fetoprotein impairs APC function and induces their apoptosis. J Immunol 173, 1772–1778. Uriel, J., Failly-Crepin, C., Villacampa, M.J., Pineiro, A., Geuskens., 1984. Incorporation of AFP by the MCF-7 human breast cancer cell line. Tumor Biol 5, 41–51. Uriel, J., Faivre-Bauman, A., Trojan, J., Doiret, D., 1981. Immunocytochemical demonstration of alpha-fetoprotein uptake by primary cultures of fetal hemisphere cells from mouse brain. Neurosci Lett 27, 171–175. Uriel, J., Trojan, J., Moro, R., Pineiro, A., 1983. Intracellular uptake of alpha-fetoprotein: a marker of neural differentiation. Ann NY Acad Sci 417, 321–329. Villacampa, M.J., Lampreave, F., Calvo, M., Naval, J., Pineiro, A., 1984. Uriel, J. Incorporation of radiolabelled alpha-fetoprotein in the brain and other tissues of the developing rat. Dev Brain Res 12, 77–82. Vollmer, C.M. Jr., Eilber, F.C., Butterfield, L.H., Ribas, A., Dissette, VB., Koh, A., Montejo, L.D., Lee, M.C., Andrews, K.J., McBride, W.H., Glaspy J.A., Economou J.S., 1999. Alpha-fetoprotein-specific genetic immunotherapy for hepatocellular carcinoma. Cancer Res 59, 3064–3067. Wald, N.J., Morris, J.K., Ibison, J., Wu, T., George, L.M., 2006. Screening in early pregnancy for pre-eclampsia using Down syndrome quadruple test markers. Prenat Diagn 26, 559–564. Walter, B.N.J., Love, T., Smith, V., De Swiet, M., 1985. Alpha-fetoprotein elevation in proteinuric pre-eclampsia. Br J Obstet Gynecol 92, 341. Watanabe, A., Miyazaki, M., Taketa, K., 1976. Differential mechanisms of increased a,-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy. Cancer Res 217, 1-5. Yamada, T., Fujita, M., Kitao, S., Ashida, Y., Nishizono, K., Tsuchiya, R., Shida, T., Kobayashi, K., 1999. Serum alphafetoprotein values in dogs with various hepatic diseases. J Vet Med Sci 61, 657–659. Yamada, T., Kakinoki, M., Totsuka, K., Ashida, Y., Nishizono, K., Tsuchiya, R., Kobayashi, K., 1995. Purification of canine alpha-fetoprotein and alpha-fetoprotein values in dogs. Vet Immunol Immunopathol 47, 25–33.
摘要: α型胎兒蛋白簡稱AFP,是一哺乳動物胎兒時期主要的血清蛋白,此蛋白是一種運輸蛋白並會參與胎兒組織的生長調節,一般認為此蛋白會經由受體媒介的胞飲作用進入細胞進而參與腫瘤細胞和胚胎細胞的生長發育。α型胎兒蛋白受體簡稱AFPR,最早於人類的MCF-7乳癌細胞株發現,並陸續在人類單核球細胞株以及其他腫瘤的細胞膜上發現此特異性受體的存在,因此AFPR逐漸被認為是一種泛腫瘤表現的膜蛋白受體。雖然AFPR已被部分分離及純化,但其特性及生理機制仍未完全明朗。然而隨著抗AFPR的單株抗體發展,已有文獻指出人類AFPR的血清量具有偵測人類早期乳腺癌的能力。另外,合併使用AFPR及CA125亦能提高早期診斷人類卵巢癌的敏感度及特異度。目前在犬AFPR血清量的相關研究甚少。因此,本實驗的目的為利用冷光化學免疫試驗偵測並定量在健康犬及各種腫瘤病犬的AFPR血清量,以及比較腫瘤患犬在治療前後血清AFPR量的變化。結果顯示在26隻健康犬AFPR血清量為4953±1220 (Mean ± SD, median 5284) units;24隻良性腫瘤病犬的AFPR血清量為4770±1256 (Mean ± SD, median 4601) units;94隻惡性腫瘤病犬的AFPR血清量為4742±1497 (Mean ± SD, median 4669) units,上述結果顯示AFPR 血清量於健康犬及腫瘤患犬間並無顯著差異。上皮來源腫瘤、間質來源腫瘤、血淋巴來源腫瘤及性腺來源腫瘤的腫瘤患犬AFPR 血清量亦未見明顯差異。此外,比較各種類別間良惡性腫瘤的AFPR血清量,僅有惡性上皮腫瘤患犬有較高的趨勢。在比較治療前後的AFPR血清量變化上發現,化療前後AFPR血清量的變化與疾病進展有明顯相關,若AFPR血清量上升則代表惡化,下降則代表好轉。然而,手術切除局部病灶的患犬並未見到此AFPR血清量的變化現象。總結,犬 AFPR血清量在區別良惡性腫瘤的效力並不顯著。然而犬AFPR血清量應能使用在全身性腫瘤病犬化療後的復發追蹤,以及用於評估惡性腫瘤的預後。然而此結果仍待更進一步研究。
Alpha-fetoprotein (AFP), assigned as an onco-fetal protein in mammalian, were single-chain glycoproteins with molecular masses ranging from 69 kDa and 3%–5% carbohydrate (glycan) content. The entry of AFP into malignant cells may occurred by a process of receptor-mediated endocytosis. The specific binding of alpha-fetoprotein was first demonstrated on human MCF-7 breast cancer cell line, and then subsequently on the membrane of various neoplastic cells. Followed, the putative alpha-fetoprotein receptor (AFPR) was partially purified and characterized from human monocytes. Several studies had been demonstrated that the presence of AFPR on tumor cell membranes. By the developing of monoclonal antibody against the AFP receptor, AFPR was considered to provide with the better sensitivity and specificity in detection for some human early-stage breast cancers than conventional serum marker. Combination of AFPR and CA-125 also elevated the efficacy on ovarian tumor detection. However, researches about AFPR in canine sera were rare. The aim of our study was to explore the difference of serum AFPR level by the quantitative chemiluminescence immunoassay in dogs with healthy condition and tumor-bearing dogs and to compare the serum AFPR values of untreated dogs with treated dogs. Results showed that there were neither a significant difference in serum AFPR level between healthy group and tumor-bearing group nor obvious differences among epithelial tumor group, mesenchymal tumor group, hematopoietic/ lymphoreticular tumor group, and gonadal tumors group. Except for the malignant epithelial tumors having higher tendency than benign epithelial tumors (p=0.098), there is no statistical difference between different types of neoplasm. Intriguingly, serum AFPR level in 14 of 15 dogs after chemotherapy showed highly corresponding along with course of disease, increased level for progression and decreased level for remission, respectively. However, there was no significant difference between serum AFPR level in those dogs with localized tumor before and after surgery. In conclusion, although serum AFPR level have unremarkable efficacy in screening and detecting canine tumors, serum AFPR level may be able to use on monitoring the dogs with the relapse of systemic malignant tumors after chemotherapy. Furthermore, AFPR level may have potential use to evaluate the prognosis of the dogs with advanced tumors but it need further investigation.
URI: http://hdl.handle.net/11455/14351
其他識別: U0005-1707201315124700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1707201315124700
Appears in Collections:獸醫學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.