Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/15273
標題: 口腔黏膜低劑量干擾素對豬免疫功能之調控及對PRRSV或PRRSV/PCV2混合感染之保護效益評估
Immunoregulation of Oromucosal Low-Dose rh-Interferon in Pigs, and the Protective Efficiency Against PRRSV and/or PCV2 infections
作者: 楊韻蓉
Yang, Yung-Rong
關鍵字: pig

interferon
low-dose
oromucosal
immunoregulation
干擾素
低劑量
口腔黏膜
免疫調節
出版社: 獸醫病理生物學研究所
引用: 黃億銘。利用流體細胞儀及Time-resolved fluorometer建立非放射性細胞毒殺作用分析法及豬瘟病毒感染與毒殺細胞交互關係之探討。國立中興大學獸醫病理學研究所碩士論文。1999。 鍾文彬、吳大中、林敏雯、黃瓊儀、張志成、張文發及楊平政。台灣地區豬繁殖與呼吸道症候群 III. 流行病學調查。中華民國獸醫學會雜誌 23: 43-50, 1997。 蔡昇達。大腸桿菌表現之第二型豬環狀病毒次單位疫苗保護效益之評估:田間試驗及人工感染試驗。國立中興大學獸醫病理學研究所碩士論文。2007。 游能凱。口服低劑量干擾素對於豬隻免疫功能之調控及對PRRSV與PCV2混合感染之保護效益。國立中興大學獸醫病理學研究所碩士論文。2010。 Ahmed R. Tickling memory T cells. Science 272: 1904, 1996. Akira S. and Takeda K. Toll-like receptor signalling. Nat. Rev. Immunol. 4: 499–511, 2004. Akira S., Uematsu S. and Tacheuchi O. Pathogenic recognition and innate immunity. Cell 124: 783–801, 2006. Albina E., Truong C., Hutet E., Blanchard P., Cariolet R., L''Hospitalier R., Mahe D., Allee C., Morvan H., Amenna N., Le Dimna M., Madec F. and Jestin A. An experimental model for post-weaning multisystemic wasting syndrome (PMWS) in growing piglets. J Comp Pathol 125: 292-303, 2001. Alexopoulos C., Kritas S. K., Kyriakis C. S., Tzika E. and Kyriakis S. C. Sow performance in an endemically porcine reproductive and respiratory syndrome (PRRS)-infected farm after sow vaccination with an attenuated PRRS vaccine. Vet Microbiol 111: 151-157, 2005. Alexopoulou L., Holt A. C., Medzhitov R. and Flavell R. A. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 413: 732–738, 2001. Allan G., McNeilly F. and McNair I. Passive transfer of maternal antibodies to PCV2 protects against development of post-weaning multisystemic wasting syndrome (PMWS): Experimental infections and a field study. Pig J 50: 59-67, 2002. Allan G. M., Caprioli A., McNair I., Lagan-Tregaskis P., Ellis J., Krakowka S., McKillen J., Ostanello F. and McNeilly F. Porcine circovirus 2 replication in colostrum-deprived piglets following experimental infection and immune stimulation using a modified live vaccine against porcine respiratory and reproductive syndrome virus. Zoonoses Public Health 54: 214-222, 2007. Allan G. M. and Ellis J. A. Porcine circoviruses: a review. J Vet Diagn Invest 12: 3-14, 2000. Allan G. M., Kennedy S., McNeilly F., Foster J. C., Ellis J. A., Krakowka S. J., Meehan B. M. and Adair B. M. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121: 1-11, 1999. Allan G. M., McNeilly F., Cassidy J. P., Reilly G. A., Adair B., Ellis W. A. and McNulty M. S. Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet Microbiol 44: 49-64, 1995. Allan G. M., McNeilly F., Ellis J., Krakowka S., Meehan B., McNair I., Walker I. and Kennedy S. Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication. Arch Virol 145: 2421-2429, 2000. Allan G. M., McNeilly F., McMenamy M., McNair I., Krakowka S. G., Timmusk S., Walls D., Donnelly M., Minahin D., Ellis J., Wallgren P. and Fossum C. Temporal distribution of porcine circovirus 2 genogroups recovered from postweaning multisystemic wasting syndrome affected and nonaffected farms in Ireland and Northern Ireland. J Vet Diagn Invest 19: 668-673, 2007. Allan G. M., Phenix K. V., Todd D. and McNulty M. S. Some biological and physico-chemical properties of porcine circovirus. Zentralbl Veterinarmed B 41: 17-26, 1994. Altrock B. W., Fagin K. D., Hockman H. R., Fish E. N., Goldstein L., Chang D., Duker K. and Stebbing N. Antiviral and antitumor effects of a human interferon analog, IFN-alpha Con 1, assessed in hamsters. J Interferon Res 6: 405-415, 1986. An T. Q., Zhou Y. J., Liu G. Q., Tian Z. J., Li J., Qiu H. J. and Tong G. Z. Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: coexistence of two NA-subgenotypes with great diversity. Vet Microbiol 123: 43-52, 2007. Andraud M., Grasland B., Durand B., Cariolet R., Jestin A., Madec F. and Rose N. Quantification of porcine circovirus type 2 (PCV-2) within- and between-pen transmission in pigs. Vet Res 39: 43, 2008. Azaman S. N., Ramakrishnan N. R., Tan J. S., Rahim R. A., Abdullah M. P. and Ariff A. B. Optimization of an induction strategy for improving interferon-alpha2b production in the periplasm of Escherichia coli using response surface methodology. Biotechnol Appl Biochem 56: 141-150, 2010. Balasch M., Segales J., Rosell C., Domingo M., Mankertz A., Urniza A. and Plana-Duran J. Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome. J Comp Pathol 121: 139-148, 1999. Balcerska A., Bohdan Z., Drozynska E., Kozielska E., Szarszewski A. and Georgiades J. A. Evaluation of the efficacy of natural human interferon alpha lozenges on the clinical course of childhood neoplasia and in chronic hepatitis B virus infection in patients who were successfully treated for pediatric malignancies. Arch Immunol Ther Exp (Warsz) 41: 221-227, 1993. Bassaganya-Riera J., Pogranichniy R. M., Jobgen S. C., Halbur P. G., Yoon K. J., O''Shea M., Mohede I. and Hontecillas R. Conjugated linoleic acid ameliorates viral infectivity in a pig model of virally induced immunosuppression. J Nutr 133: 3204-3214, 2003. Batista L., Dee S. A., Rossow K. D., Polson D. D., Xiao Z., Olin M., Murtaugh M. P., Molitor T. W., Joo H. S. and Pijoan C. Detection of porcine reproductive and respiratory syndrome virus in pigs with low positive or negative ELISA s/p ratios. Vet Rec 154: 25-26, 2004. Batista L., Pijoan C. and Torremorell M. Experimental injection of gilts with porcine reproductive and respiratory syndrome virus (PRRSV) during acclimatization. J Swine Health Prod 10: 147–150, 2002. Bautista E. M., Goyal S. M., Yoon I. J., Joo H. S. and Collins J. E. Comparison of porcine alveolar macrophages and CL 2621 for the detection of porcine reproductive and respiratory syndrome (PRRS) virus and anti-PRRS antibody. J Vet Diagn Invest 5: 163-165, 1993. Bautista E. M. and Molitor T. W. IFN gamma inhibits porcine reproductive and respiratory syndrome virus replication in macrophages. Arch Virol 144: 1191-1200, 1999. Beilharz M. W., McDonald W., Watson M. W., Heng J., McGeachie J. and Lawson C. M. Low-dose oral type I interferons reduce early virus replication of murine cytomegalovirus in vivo. J Interferon Cytokine Res 17: 625-630, 1997. Beura L. K., Sarkar S. N., Kwon B., Subramaniam S., Jones C., Pattnaik A. K. and Osorio F. A. Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. J Virol 84: 1574-1584, 2010. Biron C. A. Initial and innate responses to viral infections--pattern setting in immunity or disease. Curr Opin Microbiol 2: 374-381, 1999. Biron C. A. Interferons alpha and beta as immune regulators--a new look. Immunity 14: 661-664, 2001. Bolin S. R., Stoffregen W. C., Nayar G. P. and Hamel A. L. Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived, colostrum-deprived piglets with type 2 porcine circovirus. J Vet Diagn Invest 13: 185-194, 2001. Brassard D. L., Grace M. J. and Bordens R. W. Interferon-alpha as an immunotherapeutic protein. J Leukoc Biol 71: 565-581, 2002. Brod S. A., Kerman R. H., Nelson L. D., Marshall G. D., Henninger E. M., Khan M., Jin R. and Wolinsky J. S. Ingested IFN-α has biological effects in humans with relapsing-remitting multiple sclerosis. Mult Scler. 3: 1-7, 1997. Brunborg I. M., Jonassen C. M., Moldal T., Bratberg B., Lium B., Koenen F. and Schonheit J. Association of myocarditis with high viral load of porcine circovirus type 2 in several tissues in cases of fetal death and high mortality in piglets. A case study. J Vet Diagn Invest 19: 368-375, 2007. Buddaert W., Van Reeth K. and Pensaert M. In vivo and in vitro interferon (IFN) studies with the porcine reproductive and respiratory syndrome virus (PRRSV). Adv Exp Med Biol 440: 461-467, 1998. Buechler C., Ritter M., Orso E., Langmann T., Klucken J. and Schmitz G. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli. J. Leukoc. Biol. 67: 97–103, 2000. Bush E., Corso B., Zimmerman J., Swenson S., Pyburn D. and Burkgren T. Update on the acute PRRS investigative study. Swine Health Prod 7: 179–180, 1999. Caban J., Mossor-Ostrowska J., Zyrkowska-Bieda T., Zejc M., Janas-Skulina U., Ciesla A., Cummins J. M. and Georgiades J. A. Treatment of chronic viral hepatitis type B with oral mucosal administration of natural human interferon alpha lozenges. Arch Immunol Ther Exp (Warsz) 41: 229-235, 1993. Calsamiglia M., Fraile L., Espinal A., Cuxart A., Seminati C., Martin M., Mateu E., Domingo M. and Segales J. Sow porcine circovirus type 2 (PCV2) status effect on litter mortality in postweaning multisystemic wasting syndrome (PMWS). Res Vet Sci 82: 299-304, 2007. Calvert J. G., Slade D. E., Shields S. L., Jolie R., Mannan R. M., Ankenbauer R. G. and Welch S. K. CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. J Virol 81: 7371-7379, 2007. Cancel-Tirado S. M., Evans R. B. and Yoon K. J. Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection. Vet Immunol Immunopathol 102: 249-262, 2004. Cano J. P., Dee S. A., Murtaugh M. P. and Pijoan C. Impact of a modified-live porcine reproductive and respiratory syndrome virus vaccine intervention on a population of pigs infected with a heterologous isolate. Vaccine 25: 4382-4391, 2007. Cano J. P., Dee S. A., Murtaugh M. P., Trincado C. A. and Pijoan C. B. Effect of vaccination with a modified-live porcine reproductive and respiratory syndrome virus vaccine on dynamics of homologous viral infection in pigs. Am J Vet Res 68: 565-571, 2007. Carrasco L., Segales J., Bautista M. J., Gomez-Villamandos J. C., Rosell C., Ruiz-Villamor E. and Sierra M. A. Intestinal chlamydial infection concurrent with postweaning multisystemic wasting syndrome in pigs. Vet Rec 146: 21-23, 2000. Chang H. C., Peng Y. T., Chang H. L., Chaung H. C. and Chung W. B. Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus. Vet Microbiol 129: 281-293, 2008. Chang H. W., Jeng C. R., Lin T. L., Liu J. J., Chiou M. T., Tsai Y. C., Chia M. Y., Jan T. R. and Pang V. F. Immunopathological effects of porcine circovirus type 2 (PCV2) on swine alveolar macrophages by in vitro inoculation. Vet Immunol Immunopathol 110: 207-219, 2006. Chang H. W., Jeng C. R., Liu J. J., Lin T. L., Chang C. C., Chia M. Y., Tsai Y. C. and Pang V. F. Reduction of porcine reproductive and respiratory syndrome virus (PRRSV) infection in swine alveolar macrophages by porcine circovirus 2 (PCV2)-induced interferon-alpha. Vet Microbiol 108: 167-177, 2005. Charerntantanakul W., Platt R., Johnson W., Roof M., Vaughn E. and Roth J. A. Immune responses and protection by vaccine and various vaccine adjuvant candidates to virulent porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 109: 99-115, 2006. Chaung H. C., Chen C. W., Hsieh B. L. and Chung W. B. Toll-Like Receptor expressions in porcine alveolar macrophages and Dendritic Cells in responding to poly IC stimulation and porcine reproductive and respiratory syndrome virus (PRRSV) infection. Comp Immunol Microbiol Infect Dis 33: 197-213, 2010. Chayama K., Suzuki F., Tsubota A., Kobayashi M., Arase Y., Saitoh S., Suzuki Y., Murashima N., Ikeda K., Takahashi N., Kinoshita M. and Kumada H. Association of amino acid sequence in the PKR-eIF2 phosphorylation homology domain and response to interferon therapy. Hepatology 32: 1138-1144, 2000. Chen N., Cao Z., Yu X., Deng X., Zhao T., Wang L., Liu Q., Li X. and Tian K. Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China. J Gen Virol 92: 880-892, 2011. Chen Z., Lawson S., Sun Z., Zhou X., Guan X., Christopher-Hennings J., Nelson E. A. and Fang Y. Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398: 87-97, 2010. Cheung A. K. The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2. Virology 313: 452-459, 2003. Cheung A. K. Homologous recombination within the capsid gene of porcine circovirus type 2 subgroup viruses via natural co-infection. Arch Virol 154: 531-534, 2009. Chianini F., Majo N., Segales J., Dominguez J. and Domingo M. Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 94: 63-75, 2003. Chiarelli-Neto O., Yotoko K. S., Vidigal P. M., Silva F. M., Castro L. A., Fietto J. L., Silva A., Jr. and Almeida M. R. Classification and putative origins of Brazilian porcine circovirus 2 inferred through phylogenetic and phylogeographical approaches. Virus Res 140: 57-63, 2009. Choi C. and Chae C. In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Comp Pathol 121: 265-270, 1999. Christopher-Hennings J., Nelson E. A., Hines R. J., Nelson J. K., Swenson S. L., Zimmerman J. J., Chase C. L., Yaeger M. J. and Benfield D. A. Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of adult boars. J Vet Diagn Invest 7: 456-464, 1995. Cochet M., Vaiman D. and Lefevre F. Novel interferon delta genes in mammals: cloning of one gene from the sheep, two genes expressed by the horse conceptus and discovery of related sequences in several taxa by genomic database screening. Gene 433: 88-99, 2009. Colamonici O. R., Platanias L. C., Domanski P., Handa R., Gilmour K. C., Diaz M. O., Reich N. and Pitha-Rowe P. Transmembrane signaling by the alpha subunit of the type I interferon receptor is essential for activation of the JAK kinases and the transcriptional factor ISGF3. J Biol Chem 270: 8188-8193, 1995. Collins J. E., Benfield D. A., Christianson W. T., Harris L., Hennings J. C., Shaw D. P., Goyal S. M., McCullough S., Morrison R. B., Joo H. S. and et al. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest 4: 117-126, 1992. Conzelmann K. K., Visser N., Van Woensel P. and Thiel H. J. Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193: 329-339, 1993. Cortey M., Pileri E., Sibila M., Pujols J., Balasch M., Plana J. and Segales J. Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008. Vet J 187: 363-368, 2011. Costers S., Lefebvre D. J., Van Doorsselaere J., Vanhee M., Delputte P. L. and Nauwynck H. J. GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. Arch Virol 155: 371-378, 2010. Cregg J. M. Expression in the methylotrophic yeast Pichia pastoris. In: Fernandez J.M., Hoeffler J.P., ed. Gene Expression Systems: Using Nature for the Art of Expression. Academic Press, San Diego. . 157-191, 1999. Cummins J. M., Mock R. E., Shive B. W., Krakowka S., Richards A. B. and Hutcheson D. P. Oral treatment of transmissible gastroenteritis with natural human interferon alpha: a field study. Vet Immunol Immunopathol 45: 355-360, 1995. Cummins J. M., Tompkins M. B., Olsen R. G., Tompkins W. A. and Lewis M. G. Oral use of human alpha interferon in cats. J Biol Response Mod 7: 513-523, 1988. Darwich L., Balasch M., Plana-Duran J., Segales J., Domingo M. and Mateu E. Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J Gen Virol 84: 3453-3457, 2003. Darwich L., Pie S., Rovira A., Segales J., Domingo M., Oswald I. P. and Mateu E. Cytokine mRNA expression profiles in lymphoid tissues of pigs naturally affected by postweaning multisystemic wasting syndrome. J Gen Virol 84: 2117-2125, 2003. Darwich L., Segales J., Domingo M. and Mateu E. Changes in CD4(+), CD8(+), CD4(+) CD8(+), and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues. Clin Diagn Lab Immunol 9: 236-242, 2002. Darwich L., Segales J. and Mateu E. Pathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2: An immune riddle. Arch Virol 149: 857-874, 2004. Dec M. and Puchalski A. Use of oromucosally administered interferon-alpha in the prevention and treatment of animal diseases. Pol J Vet Sci 11: 175-186, 2008. Dee S., Joo H. S. and Pijoan C. Controlling the spread of PRRS virus in the breeding herd through management of the gilt pool. J Swine Health Prod 3: 64–69, 1995. Dee S. A., Martinez B. C. and Clanton C. Survival and infectivity of porcine reproductive and respiratory syndrome virus in swine lagoon effluent. Vet Rec 156: 56-57, 2005. Delputte P. L., Van Breedam W., Delrue I., Oetke C., Crocker P. R. and Nauwynck H. J. Porcine arterivirus attachment to the macrophage-specific receptor sialoadhesin is dependent on the sialic acid-binding activity of the N-terminal immunoglobulin domain of sialoadhesin. J Virol 81: 9546-9550, 2007. Dewey C. E., Johnston W. T., Gould L. and Whiting T. L. Postweaning mortality in Manitoba swine. Can J Vet Res 70: 161-167, 2006. Diaz I., Darwich L., Pappaterra G., Pujols J. and Mateu E. Different European-type vaccines against porcine reproductive and respiratory syndrome virus have different immunological properties and confer different protection to pigs. Virology 351: 249-259, 2006. Diaz I., Darwich L., Pappaterra G., Pujols J. and Mateu E. Immune responses of pigs after experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus. J Gen Virol 86: 1943-1951, 2005. Dokur M., Boyadjieva N. I. and Sarkar D. K. Reduction of perforin, granzyme B, and cytokine interferon gamma by ethanol in male Fischer 344 rats. Alcohol Clin Exp Res 27: 670-676, 2003. Donadeu M., Waddilove J. and Marco E. European management strategies to control postweaning multisystemic wasting syndrome. In: Proceedings of the Allen D. Leman Swine Conference, Minneapolis, USA,. 136–142, 2003. Dorr P. M., Baker R. B., Almond G. W., Wayne S. R. and Gebreyes W. A. Epidemiologic assessment of porcine circovirus type 2 coinfection with other pathogens in swine. J Am Vet Med Assoc 230: 244-250, 2007. Drew T. W., Meulenberg J. J., Sands J. J. and Paton D. J. Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus. J Gen Virol 76 ( Pt 6): 1361-1369, 1995. Drolet R., Larochelle R., Morin M., Delisle B. and Magar R. Detection rates of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and swine influenza virus in porcine proliferative and necrotizing pneumonia. Vet Pathol 40: 143-148, 2003. Duan X., Nauwynck H. J., Favoreel H. W. and Pensaert M. B. Identification of a putative receptor for porcine reproductive and respiratory syndrome virus on porcine alveolar macrophages. J Virol 72: 4520-4523, 1998. Duan X., Nauwynck H. J. and Pensaert M. B. Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV). Arch Virol 142: 2483-2497, 1997. Duan X., Nauwynck H. J. and Pensaert M. B. Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV). Vet Microbiol 56: 9-19, 1997. Dupont K., Hjulsager C. K., Kristensen C. S., Baekbo P. and Larsen L. E. Transmission of different variants of PCV2 and viral dynamics in a research facility with pigs mingled from PMWS-affected herds and non-affected herds. Vet Microbiol 139: 219-226, 2009. Dupont K., Nielsen E. O., Baekbo P. and Larsen L. E. Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time. Vet Microbiol 128: 56-64, 2008. Eckart M. R. and Bussineau C. M. Quality and authenticity of heterologous proteins synthesized in yeast. Curr Opin Biotechnol 7: 525-530, 1996. Edwards S., Moennig V. and Wensvoort G. The development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses. Vet Microbiol 29: 101-108, 1991. Eid P., Meritet J. F., Maury C., Lasfar A., Weill D. and Tovey M. G. Oromucosal interferon therapy: pharmacokinetics and pharmacodynamics. J Interferon Cytokine Res 19: 157-169, 1999. Ellis J. A., Konoby C., West K. H., Allan G. M., Krakowka S., McNeilly F., Meehan B. and Walker I. Lack of antibodies to porcine circovirus type 2 virus in beef and dairy cattle and horses in western Canada. Can Vet J 42: 461-464, 2001. Ericsson A. D. and Cummins J. M. Clinical studies of neuromuscular disease. Raum zeit 2: 26-32, 1991. Escaramis G., Carrasco J. L., Aponte J. J., Nhalungo D., Nhacolo A., Alonso P. and Ascaso C. Spatio-temporal analysis of mortality among children under the age of five in Manhica (Mozambique) during the period 1997-2005. Int J Health Geogr 10: 14, 2011. Fachinger V., Bischoff R., Jedidia S. B., Saalmuller A. and Elbers K. The effect of vaccination against porcine circovirus type 2 in pigs suffering from porcine respiratory disease complex. Vaccine 26: 1488-1499, 2008. Fang Y., Schneider P., Zhang W. P., Faaberg K. S., Nelson E. A. and Rowland R. R. Diversity and evolution of a newly emerged North American Type 1 porcine arterivirus: analysis of isolates collected between 1999 and 2004. Arch Virol 152: 1009-1017, 2007. Fenaux M., Halbur P. G., Gill M., Toth T. E. and Meng X. J. Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38: 2494-2503, 2000. Ferbas J. J., Toso J. F., Logar A. J., Navratil J. S. and Rinaldo C. R., Jr. CD4+ blood dendritic cells are potent producers of IFN-alpha in response to in vitro HIV-1 infection. J Immunol 152: 4649-4662, 1994. Finkleman F. D., Katona I. M., Morsmann T. R. and Coffman R. L. IFN-g regulates the isotype of Ig secreted furing in vivo humoral immune response. J. Immunol. 140: 1022-1027, 1988. Flores-Mendoza L., Silva-Campa E., Resendiz M., Osorio F. A. and Hernandez J. Porcine reproductive and respiratory syndrome virus infects mature porcine dendritic cells and up-regulates interleukin-10 production. Clin Vaccine Immunol 15: 720-725, 2008. Forsberg R. Divergence time of porcine reproductive and respiratory syndrome virus subtypes. Mol Biol Evol 22: 2131-2134, 2005. Fort M., Fernandes L. T., Nofrarias M., Diaz I., Sibila M., Pujols J., Mateu E. and Segales J. Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets. Vet Immunol Immunopathol 129: 101-107, 2009. Fort M., Sibila M., Allepuz A., Mateu E., Roerink F. and Segales J. Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins. Vaccine 26: 1063-1071, 2008. Fort M., Sibila M., Perez-Martin E., Nofrarias M., Mateu E. and Segales J. One dose of a porcine circovirus 2 (PCV2) sub-unit vaccine administered to 3-week-old conventional piglets elicits cell-mediated immunity and significantly reduces PCV2 viremia in an experimental model. Vaccine 27: 4031-4037, 2009. Fraile L., Crisci E., Weenberg J., Armadans M., Mendoza L., Ruiz L., Bernaus S. and Montoya M. Effect of treatment with phytosterols in three herds with porcine respiratory disease complex. . Journal of Swine Health and Production 17: 32–41, 2009. Gagnon C. A., Tremblay D. and Tijssen P. PCV2 strain variation: What does it mean? Proceedings of the American Association of Swine Veterinarians: 535-540, 2007. Genzow M., Schwartz K., Gonzalez G., Anderson G. and Chittick W. The effect of vaccination against Porcine reproductive and respiratory syndrome virus (PRRSV) on the Porcine circovirus-2 (PCV-2) load in porcine circovirus associated disease (PCVAD) affected pigs. Can J Vet Res 73: 87-90, 2009. Georgiades J. A. Effect of low dose natural human interferon alpha given into the oral cavity on the recovery time and death loss in feedlot hospital pen cattle: a field study. Arch Immunol Ther Exp (Warsz) 41: 205-207, 1993. Gibson D. M., Cotler S., Spiegel H. E. and Colburn W. A. Pharmacokinetics of recombinant leukocyte A interferon following various routes and modes of administration to the dog. J Interferon Res 5: 403-408, 1985. Gillespie J., Opriessnig T., Meng X. J., Pelzer K. and Buechner-Maxwell V. Porcine circovirus type 2 and porcine circovirus-associated disease. J Vet Intern Med 23: 1151-1163, 2009. Gillespie T. G. and Carroll A. L. Methods of control and elimination of porcine reproductive and respiratory syndrome virus using modified live vaccine in a two-site production system. J Swine Health Prod 11: 291–295, 2003. Gilpin D. F., McCullough K., Meehan B. M., McNeilly F., McNair I., Stevenson L. S., Foster J. C., Ellis J. A., Krakowka S., Adair B. M. and Allan G. M. In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system. Vet Immunol Immunopathol 94: 149-161, 2003. Grau-Roma L., Crisci E., Sibila M., Lopez-Soria S., Nofrarias M., Cortey M., Fraile L., Olvera A. and Segales J. A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet Microbiol 128: 23-35, 2008. Grau-Roma L., Hjulsager C. K., Sibila M., Kristensen C. S., Lopez-Soria S., Enoe C., Casal J., Botner A., Nofrarias M., Bille-Hansen V., Fraile L., Baekbo P., Segales J. and Larsen L. E. Infection, excretion and seroconversion dynamics of porcine circovirus type 2 (PCV2) in pigs from post-weaning multisystemic wasting syndrome (PMWS) affected farms in Spain and Denmark. Vet Microbiol 135: 272-282, 2009. Gutterman J. U. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A 91: 1198-1205, 1994. Ha Y., Ahn K. K., Kim B., Cho K. D., Lee B. H., Oh Y. S., Kim S. H. and Chae C. Evidence of shedding of porcine circovirus type 2 in milk from experimentally infected sows. Res Vet Sci 86: 108-110, 2009. Ha Y., Lee E. M., Lee Y. H., Kim C. H., Kim D., Chae S., Ahn K. K., Kim B. and Chae C. Effects of a modified live CSFV vaccine on the development of PMWS in pigs infected experimentally with PCV-2. Vet Rec 164: 48-51, 2009. Ha Y., Lee Y. H., Ahn K. K., Kim B. and Chae C. Reproduction of postweaning multisystemic wasting syndrome in pigs by prenatal porcine circovirus 2 infection and postnatal porcine parvovirus infection or immunostimulation. Vet Pathol 45: 842-848, 2008. Haasbach E., Droebner K., Vogel A. B. and Planz O. Low-Dose Interferon Type I Treatment Is Effective Against H5N1 and Swine-Origin H1N1 Influenza A Viruses In Vitro and In Vivo. J Interferon Cytokine Res, 2011. Halbur P. G. and Bush E. J. Update on abortion storms and sow mortality. Swine Health Prod 5: 73, 1997. Harding J. Postweaning multisystemic wasting syndrome: epidemiology and clinical presentation. . Swine Health Prod. 6: 249-254, 1998. Harding J. and Clark E. Recognizing and diagnosing postweaning multisystemic wasting syndrome (PMWS). J Swine Health Prod 5: 201-203, 1997. Harding J. C. The clinical expression and emergence of porcine circovirus 2. Vet Microbiol 98: 131-135, 2004. Harms P. A., Sorden S. D., Halbur P. G., Bolin S. R., Lager K. M., Morozov I. and Paul P. S. Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38: 528-539, 2001. Haruna J., Hanna P., Hurnik D., Ikede B., Miller L. and Yason C. The role of immunostimulation in the development of postweaning multisystemic wasting syndrome in pigs under field conditions. Can J Vet Res 70: 269-276, 2006. Hasslung F. C., Berg M., Allan G. M., Meehan B. M., McNeilly F. and Fossum C. Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-alpha production by porcine PBMCs. J Gen Virol 84: 2937-2945, 2003. Hendrzak J. and Brunda M. Biology of disease: interleukin-12, biologic activity, therapeutic utility and role in disease. Lab. Investig. 72: 619-639, 1995. Heng J. K., Price P., Lai C. M. and Beilharz M. W. Alpha/beta interferons increase host resistance to murine AIDS. J Virol 70: 4517-4522, 1996. Hesse R., Kerrigan M. and Rowland R. R. Evidence for recombination between PCV2a and PCV2b in the field. Virus Res 132: 201-207, 2008. Hirose O., Shibata I., Kudou H., Samegai Y., Yoshizawa S., Ono M., Nishimura M., Hiroike T., Kageyama K. and Sakano T. Experimental infection of SPF piglets with porcine reproductive and respiratory syndrome (PRRS) viruses isolated from two farms. J Vet Med Sci 57: 991-995, 1995. Hodgson J. Expression systems: a user''s guide. Emphasis has shifted from the vector construct to the host organism. Biotechnology (N Y) 11: 887-893, 1993. Hoofnagle J. H. and Everhart J. Dose-finding study of interferon alfa-n3 in hepatitis C. Hepatology 26: 1366-1367, 1997. Horlen K. P., Dritz S. S., Nietfeld J. C., Henry S. C., Hesse R. A., Oberst R., Hays M., Anderson J. and Rowland R. R. A field evaluation of mortality rate and growth performance in pigs vaccinated against porcine circovirus type 2. J Am Vet Med Assoc 232: 906-912, 2008. Hutchinson V. and Cummins J. M. Low-dose oral interferon in patient with AIDS. Lancet 2: 1530-1531, 1987. Isaacs A. and Lindenmann J. Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci 147: 258-267, 1957. Johnson C. R., Yu W. and Murtaugh M. P. Cross-reactive antibody responses to nsp1 and nsp2 of Porcine reproductive and respiratory syndrome virus. J Gen Virol 88: 1184-1195, 2007. Jung K., Alekseev K. P., Zhang X., Cheon D. S., Vlasova A. N. and Saif L. J. Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. J Virol 81: 13681-13693, 2007. Jung K., Renukaradhya G. J., Alekseev K. P., Fang Y., Tang Y. and Saif L. J. Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J Gen Virol 90: 2713-2723, 2009. Kadowaki N., Antonenko S., Lau J. Y. and Liu Y. J. Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J Exp Med 192: 219-226, 2000. Karti S. S., Ovali E., Ratip S., Cetiner M., Direskeneli H., Bayik M. and Akoglu T. Effect of interferon-alpha(2a) on neutrophil adhesion and phagocytosis in chronic myeloid leukemia and Behcet''s disease. Clin Rheumatol 21: 211-214, 2002. Katze M. G. Interferon, PKR, virology, and genomics: what is past and what is next in the new millennium? J Interferon Cytokine Res 22: 283-286, 2002. Kawai T. and Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol. 11: 373–384, 2010. Keffaber K. K. Reproductive failure of unknown etiology., 1989. Kekarainen T., McCullough K., Fort M., Fossum C., Segales J. and Allan G. M. Immune responses and vaccine-induced immunity against Porcine circovirus type 2. Vet Immunol Immunopathol 136: 185-193, 2010. Kekarainen T., Montoya M., Dominguez J., Mateu E. and Segales J. Porcine circovirus type 2 (PCV2) viral components immunomodulate recall antigen responses. Vet Immunol Immunopathol 124: 41-49, 2008. Kekarainen T., Montoy
摘要: PCV2相關疾病(porcine circovirus-associated diseases, PCVAD) 是由複雜的多重致病因子共同引發之疾病,其主要病原為豬第二型環狀病毒(porcine circovirus type 2, PCV2),常與豬繁殖與呼吸道症候群病毒(porcine reproductive and respiratory syndrome virus, PRRSV) 混合感染及其他危險因子激發下而加劇本病之發生。除PCV2疫苗外,良好的環境與管理及適當免疫調節劑之應用,有助於PCVAD及呼吸道疾病之控制。本試驗擬探討低劑量經口腔黏膜給予recombinant human interferon-alpha (rhIFN-α,對豬隻免疫之調解能力及抗PRRSV/PCV2病毒能力進行評估。免疫試驗選用一般商業用離乳豬,每日經口腔黏膜給予低劑量rhIFN-α (10 IU/kg.bw) 並持續1週,分別在投予後之第4、8及15天進行白血球功能分析,結果顯示低劑量rhIFN-α可增加白血球總數、增強NK cell細胞毒殺能力及淋巴細胞對分裂原之致活能力,但對嗜中性球之吞噬及自由基產生,並無明顯影響。試驗二選用相同豬場來源的豬隻提早離乳並隔離飼養於獨立試驗場所,將其經胎次、性別與體重逢機配對後均分為五組(n = 6),分別為rhIFN-α投予組 (A、B及C組) 及對照組(D及E組),A 組為陰性對照組,B、C、D及E組均於7週齡進行PRRSV人工感染(104.9 TCID50/50 μL),C組及E組另外於8週進行PCV2攻毒(104.7 TCID50/50 μL)。A、B及C組豬隻在3週齡、6齡及9週齡時,經口腔黏膜給予rhIFN-α (10 IU/kg.bw) 並持續1週,所有試驗豬於11週齡犧牲。結果顯示經口腔黏膜連續給予低劑量rhIFN-α可減輕豬隻混合感染PRRSV/PCV2引起之發燒、改善試驗豬生長性能、延遲PRRSV血清抗體轉陽及降低混合感染對肺臟的損傷。以上結果亦顯示在低毒力病毒(PRRSV/PCV2) 混合感染下,只要配合良好的飼養環境、較少的飼養緊迫,在適當時間給予抗生素降低細菌性感染的風險,並且利用低劑量IFN-α提升豬隻的免疫功能,就能降低對豬隻感染的傷害。
Porcine circovirus-associated diseases (PCVAD) is a multifactor disease syndrome, in which porcine circovirus type 2 (PCV2) has been regarded as an essential causative agent and porcine reproductive and respiratory syndrome virus (PRRSV) plays the most common synergic pathogen to trigger PCVAD. Besides PCV2 vaccination, environmental and managerial improvement, and elevation of innate immunity may be also very important in control PCVAD and respiratory infections. The study objectives were to evaluate the efficacy of oromucosal administration of low-dose recombinant human interferon-alpha (rhIFN-α on the immunomodulation and the protective efficiency against PRRSV and/or PCV2 infection in conventional pigs. Weaning pigs were oromucosally administered with rhIFN-α (10 IU/kg.bw) for 1 week and leukocyte functional assays were evaluated at day 4, 8, and 15 post treatment. The results displayed that low-dose of rhIFN-α could positively modulate the complete blood count (CBC), NK cell cytotoxicity and the lymphoproliferative response, but seemed to have a inhibitory effect on the neutrophilic phagocytosis. In challenge test, pigs were weaned at 3 weeks old and randomly divided into 5 groups (n = 6 each group), including unchallenged with IFN-treatment, and IFN-treatment or placebo-treatmentfollowed with PRRSV challenged only or PRRSV/PCV2 co-infections. Low-dose of rhIFN-α was treated oromucosally at 3, 6, and 9 weeks old for 1 week. PRRSV was inoculated at 7 weeks old at the dose of 104.9 TCID50/50 μL, following PCV2 challenged at 8 weeks old at the dose of 104.7 TCID50/50 μL. All experimented pigs were sacrificed at 11 weeks old. The results indicated that low-dose IFN-α oromucosally may decrease the severity of clinical syndromes, growth performance, pulmonary impairment and lesions of lymphoid tissues, and delay seroconversion of PRRSV-specific antibody response after PRRSV infection. In view of this, adequate environment and managerial improvement with low-dose rhIFN-α administration could positively modulate the host immunity against pulmonary infections.
URI: http://hdl.handle.net/11455/15273
其他識別: U0005-1108201109235200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1108201109235200
Appears in Collections:獸醫病理生物學所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.