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標題: 口腔黏膜低劑量干擾素對豬免疫功能之調控及對PRRSV或PRRSV/PCV2混合感染之保護效益評估
Immunoregulation of Oromucosal Low-Dose rh-Interferon in Pigs, and the Protective Efficiency Against PRRSV and/or PCV2 infections
作者: 楊韻蓉
Yang, Yung-Rong
關鍵字: pig

出版社: 獸醫病理生物學研究所
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摘要: PCV2相關疾病(porcine circovirus-associated diseases, PCVAD) 是由複雜的多重致病因子共同引發之疾病,其主要病原為豬第二型環狀病毒(porcine circovirus type 2, PCV2),常與豬繁殖與呼吸道症候群病毒(porcine reproductive and respiratory syndrome virus, PRRSV) 混合感染及其他危險因子激發下而加劇本病之發生。除PCV2疫苗外,良好的環境與管理及適當免疫調節劑之應用,有助於PCVAD及呼吸道疾病之控制。本試驗擬探討低劑量經口腔黏膜給予recombinant human interferon-alpha (rhIFN-α,對豬隻免疫之調解能力及抗PRRSV/PCV2病毒能力進行評估。免疫試驗選用一般商業用離乳豬,每日經口腔黏膜給予低劑量rhIFN-α (10 IU/ 並持續1週,分別在投予後之第4、8及15天進行白血球功能分析,結果顯示低劑量rhIFN-α可增加白血球總數、增強NK cell細胞毒殺能力及淋巴細胞對分裂原之致活能力,但對嗜中性球之吞噬及自由基產生,並無明顯影響。試驗二選用相同豬場來源的豬隻提早離乳並隔離飼養於獨立試驗場所,將其經胎次、性別與體重逢機配對後均分為五組(n = 6),分別為rhIFN-α投予組 (A、B及C組) 及對照組(D及E組),A 組為陰性對照組,B、C、D及E組均於7週齡進行PRRSV人工感染(104.9 TCID50/50 μL),C組及E組另外於8週進行PCV2攻毒(104.7 TCID50/50 μL)。A、B及C組豬隻在3週齡、6齡及9週齡時,經口腔黏膜給予rhIFN-α (10 IU/ 並持續1週,所有試驗豬於11週齡犧牲。結果顯示經口腔黏膜連續給予低劑量rhIFN-α可減輕豬隻混合感染PRRSV/PCV2引起之發燒、改善試驗豬生長性能、延遲PRRSV血清抗體轉陽及降低混合感染對肺臟的損傷。以上結果亦顯示在低毒力病毒(PRRSV/PCV2) 混合感染下,只要配合良好的飼養環境、較少的飼養緊迫,在適當時間給予抗生素降低細菌性感染的風險,並且利用低劑量IFN-α提升豬隻的免疫功能,就能降低對豬隻感染的傷害。
Porcine circovirus-associated diseases (PCVAD) is a multifactor disease syndrome, in which porcine circovirus type 2 (PCV2) has been regarded as an essential causative agent and porcine reproductive and respiratory syndrome virus (PRRSV) plays the most common synergic pathogen to trigger PCVAD. Besides PCV2 vaccination, environmental and managerial improvement, and elevation of innate immunity may be also very important in control PCVAD and respiratory infections. The study objectives were to evaluate the efficacy of oromucosal administration of low-dose recombinant human interferon-alpha (rhIFN-α on the immunomodulation and the protective efficiency against PRRSV and/or PCV2 infection in conventional pigs. Weaning pigs were oromucosally administered with rhIFN-α (10 IU/ for 1 week and leukocyte functional assays were evaluated at day 4, 8, and 15 post treatment. The results displayed that low-dose of rhIFN-α could positively modulate the complete blood count (CBC), NK cell cytotoxicity and the lymphoproliferative response, but seemed to have a inhibitory effect on the neutrophilic phagocytosis. In challenge test, pigs were weaned at 3 weeks old and randomly divided into 5 groups (n = 6 each group), including unchallenged with IFN-treatment, and IFN-treatment or placebo-treatmentfollowed with PRRSV challenged only or PRRSV/PCV2 co-infections. Low-dose of rhIFN-α was treated oromucosally at 3, 6, and 9 weeks old for 1 week. PRRSV was inoculated at 7 weeks old at the dose of 104.9 TCID50/50 μL, following PCV2 challenged at 8 weeks old at the dose of 104.7 TCID50/50 μL. All experimented pigs were sacrificed at 11 weeks old. The results indicated that low-dose IFN-α oromucosally may decrease the severity of clinical syndromes, growth performance, pulmonary impairment and lesions of lymphoid tissues, and delay seroconversion of PRRSV-specific antibody response after PRRSV infection. In view of this, adequate environment and managerial improvement with low-dose rhIFN-α administration could positively modulate the host immunity against pulmonary infections.
其他識別: U0005-1108201109235200
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