Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/15355
標題: 以SDH及COX組織化學法對犬隻傳染性花柳性腫瘤染色
Staining the Transmissible Venereal Tumor by Succinate Dehydrogenase (SDH) and Cytochrome C Oxidase (COX) Histochemical Methods
作者: 郭哲瑄
Kuo, Che-Hsuan
關鍵字: Transmissible Venereal Tumor
傳染性花柳性腫瘤
Mitochondrial cytochemical stain
粒線體組織化學染色
出版社: 獸醫病理生物學研究所
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摘要: 犬隻傳染性花柳性腫瘤 (Transmissible venereal tumor, TVT )是台灣常見的圓形細胞腫瘤之一,腫瘤細胞可經由犬隻之間的社交互動行為及性行為所傳染。好發於外生殖器黏膜及口吻部,常見於流浪犬隻。由於其具有同種異體移殖(Allograft)的特性,近年來有學者將其運用於腫瘤幹細胞的研究。 此腫瘤於臨床診斷方面,由於細胞型態與淋巴瘤、組織細胞瘤、肥大細胞瘤等其他圓形細胞瘤非常相似,尤其在非生殖器官發生或發生轉移的病例不易鑑別。在之前的研究中,學者於細胞超顯微結構下發現犬隻傳染性花柳性腫瘤細胞內有許多粒線體,而淋巴細胞及淋巴腫瘤細胞僅含少量粒線體等結果。然而由於進行免疫細胞化學染色及製做電子顯微鏡樣本以判讀細胞超顯微結構較為費時且成本較高,因此我們於冷凍切片下對於粒線體的進行組織化學染色法,來標定犬隻傳染性花柳性腫瘤。 實驗結果顯示,犬隻傳染性花柳性腫瘤於冷凍切片下,可以被Succinate dehydrogenase組織化學染色法標定為陽性,淋巴組織則是呈現陰性至弱陽性的染色反應,肝細胞呈現細胞質強陽性、細胞核陰性的結果;而Cytochrome c oxidase 組織化學染色法則標定出犬隻傳染性花柳性腫瘤的細胞質呈現陽性染色、而細胞核呈現陰性染色的反應,淋巴組織則是呈現陰性至弱陽性的染色反應,肝細胞呈現細胞質強陽性、細胞核陰性的結果。此外,犬隻傳染性花柳性腫瘤於細胞抹片下,Succinate dehydrogenase及Cytochrome c oxidase兩種組織化學染色法皆標定出細胞質呈現陽性染色、而細胞核呈現陰性染色的結果,肝細胞呈現細胞質強陽性、細胞核陰性的結果。由以上結果可知,Succinate dehydrogenase及Cytochrome c oxidase兩種組織化學染色法,可以快速、便利且經濟的協助犬隻傳染性花柳性腫瘤的診斷,以評估其預後狀況。
Canine transmissible venereal tumor is a common round cell tumor in Taiwan. It is transmitted from dog to dog by social behavior and coition, and occurred frequently in extragenital sites and muzzle in stray dogs. Some scholars have employed this tumor cell's stem cell research because of its allograft activity. It is not easy to diagnose because it is similar to lymphoma, histiocytoma, mast cell tumor and other round cell tumor in morphology, especially in metastasis and non genital cases. In the previous studies, it was found that there were more mitochondria in transmissible venereal tumor cells than in lymphocytes and lymphoma cells. However, it costs money and takes time to do immunohistochemical stain or transmission electron microscopic examination; therefore, we used histochemical methods to label mitochondria of canine transmissible venereal tumor cells in frozen section. The results showed that transmissible venereal tumor cells appeared positive reaction of Succinate dehydrogenase (SDH) histochemical stain in the frozen sections; however, lymphoid tissues showed negative to weak reaction. Besides, hepatocytes showed strong positive reaction in cytoplasms, but negative reaction in nucleus. On the other hand, the Cytochrome C oxidase (COX) histochemical stain, showed positive reaction in transmissible venereal tumors cytoplasms, but negative in nucleus; nevertheless, lymphoid tissues showed negative to weak reaction. Furthermore, hepatocyte showed strong positive reaction in cytoplasms, but negative in nucleus. In addition, the results of transmissible venereal tumor in stamp smears showed positive reaction in cytoplasms both by Succinate dehydrogenase and Cytochrome C oxidase histochemical stain, but negative reaction in nucleus; however, hepatocyte showed strong positive in cytoplasm, but negative reaction in nucleus. Based on the results, Succinate dehydrogenase and Cytochrome C oxidase histochemical stain can assist the differential diagnosis of transmissible venereal tumor, and provide a rapid, convenient and economical method to evaluate the prognosis of animals.
URI: http://hdl.handle.net/11455/15355
其他識別: U0005-2506201213535800
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