Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/16652
標題: 整合可拋棄式超微電極的毛細電泳晶片於電分析之應用
Disposable ultramicroelectrodes integrated with a capillary electrophoresis microchip for electroanalysis
作者: 呂育昇
Liu, Yu-Sheng
關鍵字: ultramicroelectrode
超微電極
capillary electrophoresis
microchip
電泳
晶片
出版社: 化學系所
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摘要: 本實驗使用簡易操作、製作容易之微影製程技術,經由光阻SU-8 2050 旋轉塗佈、曝光、顯影等過程,得到一流道寬度為100 μm、高度55.6 μm、有效分離長度45 mm之結構良好的 PDMS 母模,翻模後以可逆接合方式製作出 (PDMS)/glass 材質之微流體晶片。此裝置與可拋棄式網版超微電極結合成為一毛細電泳整合電化學之分析系統,此新開發之微流體裝置具有可拋棄式、成本低、製程快速與對位便利之優勢。在晶片電泳分析上以多巴胺、鄰-苯二酚作為標準樣品,由實驗可得單一樣品100 μM dopamine 最佳參數條件為0.6 V偵測電位;250 V/cm分離電場強度;100 μm 對位間距;10 秒進樣時間。最後,在未來期望可應用在各種生醫樣品的偵測。
We describe here a poly(dimethylsiloxane) (PDMS)/glass microchip easily fabricated by using lithographic techniques. The CE system coupled with a disposable screen-printed ultramiroelectrodes for electroanalysis. The microchip can mass fabricated by the PDMS replica which spin-coating by SU-8 2050 photoresist on the glass substrate and solidified by exposing to UV light. The channel size is 100 μm in width and 55.6 μm in height and 45 mm long in separation length. We detect 100 μM dopamine and 100 μM catechol as model analytes on microchip electrophoresis for electroanalysis. Our optimum conditions for 100 μM dopamine are detection potential 0.6 V ; separation voltage 250 V/cm ; 10 second injection time ; 100 μm spacing, respectively. Such miniaturized devices provides the advantages of portability, sample/solvent consumption, low cost and high speedy procedure. We anticipate that the device can be applied to various biological samples in future .
URI: http://hdl.handle.net/11455/16652
其他識別: U0005-0708200809580400
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