Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/16720
標題: 液相層析質譜術於動物組織中硝基呋喃代謝物之偵測
Determination of nitrofuran metabolites in animal tissue by liquid chromatography-tandem mass spectrometry
作者: 姚佳君
Yao, Chia-Chun
關鍵字: liquid chromatography-tandem mass spectrometry
硝基呋喃
nitrofuran
animal tissue
代謝物
動物組織
出版社: 化學系所
引用: [1] WHO Technical Report Series 832: Evaluation of certain Veterinary Drug Residues in Food, Fortieth report of the Joint FAO/WHO Expert Committee on Food Additives (1993) 32. [2] European Union Commission Regulation 1442/95, Official Journal of the European Union. L143 (1995) 26. [3] Federal Register. Topical Nitrofurans; Extralabel Animal Drug Use; Order of Prohibition. Fed. Regist.67 (2002) 5470. [4] 行政院衛生署,動物用藥殘留標準,97.03.17衛署食字第0970400541號令修正,2008。 [5] R. J. McCracken, D. G. Kennedy, Determination of the furazolidone metabolite, 3-amino-2-oxazolidinone, in porcine tissues using liquid chromatography-thermospray mass spectrometry and the occurrence of residues in pigs produced in Northern Ireland, Journal of Chromatography B 691(1997) 87. [6] J. N. Delgado, Ph. D., W. A. Remers, Ph.D.,Textbook of Organic Medicinal and Pharmaceutical Chemistry, Ninth Edition, J. B. Lippincott Company. [7] 陳信志,賈東明,許正忠,邱再預,周秀冠,鄭守訓,水產品中動物用藥硝基呋喃代謝物之殘留量調查,藥物食品檢驗局調查研究年報. 26 (2008) 306-321. [8] L.A.P. Hoogenboom, G.D. van Bruchem, K. Sonne, I.C. Enninga, J.A. van Rhijn, H. Heskamp, M.B.M. Huveneers-Oorsprong, J.C.M. van der Hoeven, H.A. Kuiper, Absorption of a mutagenic metabolite released from protein-bound residues of furazolidone, Environmental Toxicology and Pharmacology 11 (2002) 273. [9] R. J. McCracken, M. A. McCoy, D. Glenn Kennedy, The prevalence and possible causes of bound and extractable residues of the furazolidone metabolite 3-amino-2-oxazolidinone in porcine tissues, Food Additives and Contaminants 14 (1997) 287. [10] European Union Commission Regulation 71/17, Official Journal of the European Union, (2003) 764. [11] Some Food Additives, Feed Additives and Naturally Occurring Substances, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 31(1983) [12] OveraIl Evaluations of Carcinogenicity: An Updating of IARC Monographs, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Supplement 7. (1987) [13] M. O’Keeffe, A. Conneely, K.M. Cooper, D.G. Kennedy, L. Kovacsics, A. Fodor, P.P.J. Mulder, J.A. van Rhijn, G. Trigueros, Nitrofuran antibiotic residues in pork The FoodBRAND retail survey, Analytica Chimica Acta 520 (2004) 125. [14] P. -S. Chu, M. I. Lopez, Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans, Journal of Agricultural Food Chemistry 53 (2005) 8934. [15] E. Verdon, P. Couedor, P. Sanders, Multi-residue monitoring for the simultaneous determination of five nitrofurans (furazolidone, furaltadone, nitrofurazone, nitrofurantoine, nifursol) in poultry muscle tissue through the detection of their five major metabolites (AOZ, AMOZ, SEM, AHD, DNSAH) by liquid chromatography coupled to electrospray tandem mass spectrometry—In-house validation in line with Commission Decision 657/2002/EC, Analytica Chimica Acta 586 (2007) 336. [16] C. Bock, P. Gowik, C. Stachel, Matrix-comprehensive in-house validation and robustness check of a confirmatory method for the determination of four nitrofuran metabolites in poultry muscle and shrimp by LC–MS/MS, Journal of Chromatography B 856 (2007) 178. [17] 食品中動物用藥殘留量檢驗方法-硝基呋喃代謝物之檢驗,96 年7 月18 日署授食字第0961800218 號公告。 [18] J. K. Finzi, J. L. Donato, M.Sucupira, G. De Nucci, Determination of nitrofuran metabolites in poultry muscle and eggs by liquid chromatography-tandem mass spectrometry, Journal of Chromatography B 824 (2005) 30. [19] M. I. Lopez, M.F. Feldlaufer, A.D. Williams, P.-S. Chu, Determination and Confirmation of Nitrofuran Residues in Honey Using LC-MS/MS, Journal of Agricultural Food Chemistry 55 (2007) 1103. [20] A. Leitner, P. Zőllner, W. Lindner, Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography–tandem mass spectrometry, Journal of Chromatography A 939 (2001) 49. [21] P. Mottier, S. -P. Khong, E. Gremaud, J. Richoz, T. Delatour, T. Goldmann, P. A. Guy, Quantitative determination of four nitrofuran metabolites in meat by isotope dilution liquid chromatography–electrospray ionisation–tandem mass spectrometry, Journal of Chromatography A 1067 (2005) 85. [22] P. -S. Chu, M. I. Lopez, Determination of Nitrofuran Residues in Milk of Dairy Cows Using Liquid Chromatography-Tandem Mass Spectrometry, Journal of Agricultural Food Chemistry 55 (2007) 2129. [23] C. Chang, D.-P. Peng, J.-E. Wu, Y.-L. Wang, and Z.-H.Yuan, Development of an Indirect Competitive ELISA for the Detection of Furazolidone Marker Residue in Animal Edible Tissues, Journal of Agricultural Food Chemistry 56 (2008) 1525. [24] P. Viñas, N. Campillo, L. Carrasco, M. Hernández-Córdoba, Analysis of Nitrofuran Residues in Animal Feed Using Liquid Chromatography and Photodiode-Array Detection, Chromatographia 65 (2007) 85. [25] A. Conneely, A. Nugent, M. O’Keeffe, Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues, Analyst 127 (2002) 705. [26] S. Chumanee, S. Sutthivaiyakit, P. Sutthivaiyakit, New Reagent for Trace Determination of Protein-Bound Metabolites of Nitrofurans in Shrimp Using Liquid Chromatography with Diode Array Detector, Journal of Agricultural Food Chemistry 57 (2009) 1752. [27] H. M. Kingston, Lois B. Jassie, Introduction to Microwave Sample Preparation, Washington, DC: America Chemical Socirty, 1998, 7-31. [28] 陳崇宇、李茂榮,微波萃取技術之應用,科儀新知,第二十五卷,第二期,行政院國家科學委員會精密儀器發展中心出版,民國九十二年。 [29] 魏銘琪,開發微波輔助頂空固相微萃取技術結合氣相層析法分析半揮發性有機汙染物之研究,國立中興大學化學系博士學位論文,民國九十二年。 [30] 周繼棋,質譜術於藥物及其代謝物和環境汙染物之分析,國立中興大學化學系博士學位論文,民國九十四年。 [31] 蔡鳳婕,質譜術於尿中微量利尿劑與化妝品中添加劑之分析研究,國立中興大學化學系博士學位論文,民國九十七年。 [32] C. Dass, Fundamentals of contemporary mass spectrometry. John Wiley & Sons (2007). [33] H-ESI Probe User Guide (2008). [34] Finnigan ™ TSQ ® Quantum Discovery, APPI/APCI Combination Probe Operator’s Manual. [35] W. M. A. Niessen, Liquid chromatography-mass spectrometry, Taylor & Framcis (2006). [36] 李茂榮,串聯質譜法(MS/MS)之原理與應用,科儀新知,第二十五卷,第二期,行政院國家科學委員會精密儀器發展中心出版。 [37] 李茂榮,低能量碰撞誘導解離之串聯質譜儀,質譜分析技術專輯,行政院國家科學委員會精密儀器發展中心編印,民國九十一年。 [38] E. de Hoffmann, V. Stroobant, Mass spectrometry: principles and applications, Third Edition, John Wiley & Sons (2002). [39] P. H. Dawson, Quadrupole mass spectrometry and its applications, American Institute of Physics (1995). [40] K. J. McHal, N. Duczak, Applying Highly-Selective Reaction Monitoring (H-SRM) for the Assay of Midazolam and 1-Hydroxymidazolam in Plasma on the TSQ Quantum Ultra, Thermo Scientific Application Note 347. [41] Y. Yamagishi, Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery, Thermo Scientific Application Note 355. [42] V. Pucci, F. Bonelli, E. Monteagudo, R. Laufer, Enhanced mass resolution method development, validation and assay application to support preclinical studies of a new drug candidate, Rapid Communications in mass Spectrometry 20 (2006) 1240. [43] X. Xu, J. Veals, W. A. Korfmacher, Comparison of conventional and enhanced mass resolution triple-quadrupole mass spectrometers for discovery bioanalytical applications, Rapid Communications in mass Spectrometry 17 (2003) 832. [44] L. D. Vainchtein, H. Rosing, D. Mirejovsky, V. Huynh, L. Lenaz, Jan H. M. Schellens, Jos H. Beijnen, Enhanced resolution triple-quadrupole mass spectrometry for ultra-sensitive and quantitative analysis of the investigational anticancer agent EO9 (apaziquone) and its metabolite EO5a in human and dog plasma to support (pre)-clinical studies of EOquin1 given intravesically, Rapid Communications in mass Spectrometry 22 (2008) 462. [45] 洪晟瀚,吹氣輔助頂空固相微萃取結合氣相層析質譜術於水中硝基多環芳香烴之研究,國立中興大學化學系碩士學位論文,民國九十七年。 [46] C.-T. Huang, Y.-Y. Su, Y.-Z. Hsieh, Optimization of the headspace solid-phase microextraction for determination of glycol ethers by orthogonal array designs, Journal of Chromatography A 977 (2002) 9. [47] F.-Y. Du, X.-H. Xiao, G.-K. Li, Application of ionic liquids in the microwave-assisted extraction of trans-resveratrol from Rhizma Polygoni Cuspidati, Journal of Chromatography A 1140 (2007) 56. [48] L.-W. Chung, K.-L. Lin, T. C.-C. Yang, M.-R. Lee, Orthogonal array optimization of microwave-assisted derivatization for determination of trace amphetamine and methamphetamine using negative chemical ionization gas chromatography–mass spectrometry Journal of Chromatography A 1216 (2009) 4083.
摘要: 本實驗使用微波輔助衍生化法結合液相層析質譜,利用加熱式電灑游離法,以高解析選擇反應偵測模式為定量方式,進行動物組織中硝基呋喃代謝物偵測。本實驗偵測四種硝基呋喃代謝物包含Semicarbazide hydrochloride(SC)、3-amino-2-oxazolidinone(AOZ)、1-aminohydantoin hydrochloride(AH)、3-amino-5-morpholinomethyl- 2-oxazolidinone(AMOZ)。實驗探討微波功率、微波時間、衍生化試劑體積、萃取溶劑種類以及萃取溶劑體積對衍生化效果之影響,並以正交設計表找出實驗最佳化參數。並探討此技術之線性範圍、偵測極限、回收率及再現性。 實驗結果顯示當微波功率於250瓦,微波時間為7秒,衍生化試劑體積為300 μL,並以乙酸乙酯5 mL為萃取溶劑條件下,可得到最佳實驗結果。將此技術應用於真實肉類樣品,於加熱式電灑游離法以及高解析選擇反應偵測模式可得到線性範圍在0.3 ng/g-50 ng/g (SC為0.5ng/g-50ng/g)之間。偵測極限在2.9-11.7 pg/g之間,線性相關係數在0.995以上。將此方法應用於真實雞肉樣品可偵測到15.6 ng/g之SC含量,故本技術可做為動物組織樣品中硝基呋喃代謝物確認技術之依據。
The aim of this study is to develop a rapid microwave-assisted derivatization method combined with liquid chromatography-tandem mass spectrometry (LC-MS-MS) to determine of four nitrofuran metabolites in animal's tissue. The derivatization parameters including microwave power, microwave time, volume of derivatization reagent and extraction solvent were studied by using a uniform experimental design at four levels. The optimal conditions obtained were at irradiation of 250W microwave for 7s and ethyl acetate used as extraction reagent. The method was performed by the heated electrospray ionization (H-ESI) and highly-selective reaction monitoring (H-SRM) to obtain high ionization efficiency and reduce the interference from the animal's tissue. The linear range was 0.3-50 ng/g (SC was 0.5-50 ng/g).The precisions (RSD%) were obtained in the range of 2-12% at 2 ng g-1 and 5-15% at 40 ng g-1. The LODs ranged between 2.9-11.7 pg g-1. The LOQs ranged between 9.6-39.1 pg g-1.The proposed method was also successfully applied to the analysis of trace nitrofuran metabolites in animal's tissue.
URI: http://hdl.handle.net/11455/16720
其他識別: U0005-1007200911053600
Appears in Collections:化學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.