Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/16861
標題: Determination of tetramethylammonium hydroxide in mouse serum by micro solid-phase extraction combined with liquid chromatography tandem mass spectrometry
微量固相萃取結合液相層析串聯質譜術於老鼠血清中氫氧化四甲基銨之檢測
作者: 林佳穎
Lin, Chia-Ying
關鍵字: tetramethylammonium hydroxide
氫氧化四甲基銨
SPE
matrix effect
LC-MS/MS
微量固相萃取
基質效應
液相層析串聯質譜術
出版社: 化學系所
引用: 1.National Fire Protection Association (NFPA) http://www.nfpa.org/ 2.吳政龍、蘇世斌、牛柯琪,氫氧化四甲基銨暴露致死亡個案報告與相關文獻回顧,奇美醫院研究報告。 3.勞工安全衛生研究所,工安警訊,“要命!勞工不慎遭氫氧化四甲基銨溶液噴濺中毒致死案例” (2007) 4.C.-L. Wu, S.-B. Su, J.-L. Chen, H.-J. Lin, H.-R. Guo, Journal of Occupational Health, 50 (2008) 99−102 5.A. J. Henry, British Journal of Pharmcology, 3 (1948) 187−188 6.K. Shiomi, M. Mizukami, K. Shimakura, Y. Nagashima, Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 107 (1994) 427−432 7.A. J. Power, B. F. Keegan, K. Nolan, Toxicon, 40 (2002) 419−425 8.D. J. West, E. B. Andrews, D. Bowman, A. R. McVean, M. C. Thorndyke, Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 113 (1996) 1−10 9.H. Tsubaki, T. Komai, Journal of Pharmacobio−Dynamics, 9 (1986) 747−754 10.J.-Y. Zhao, P. Thibault, T. Tazawa, M. A. Quilliam, Journal of Chromatography A, 781 (1997) 555−564 11.Y. Kawashima, Y. Nagashima, K. Shiomi, Toxicon, 44 (2004) 185−191 12.U. Anthoni, C. Christophersen, P. H. Nielsen, Journal of Agricultural and Food Chemistry, 37 (1989) 705−707 13.M. M. Ariffin, R. A. Anderson, Journal of Chromatography B, 842 (2006) 91–97 14.U. Anthoni, L. Bohlin, C. Larsen, P. Nielsen, N. H. Nielsen, C. Christophersen, Toxicon, 27 (1989) 717−723 15.K. Sato, M. Shikida, T. Yamashiro, K. Asaumi, Y. Iriye, M. Yamamoto, Sensors and Actuators A: Physical, 73 (1999) 131−137 16.M. Shikida, T. Masuda, D. Uchikawa, K. Sato, Sensors and Actuators A: Physical, 90 (2001) 223−231 17.O. Tabata, R. Asahi, H. Funabashi, K. Shimaoka, S. Sugiyama, Sensors and Actuators A: Physical, 34 (1992) 51−57 18.M. Kramkowska, I. Zubel, Procedia Chemistry, 1 (2009) 774−777 19.J. Throck Watson, O. David Sparkman, Introduction to Mass Spectrometry, John Wiley & Sons (2007) 173−220 20.Fred W. McLafferty, Accounts of Chemical Research, 13 (1980) 33−39 21.李茂榮,串聯質譜法(MS/MS)之原理與應用,科儀新知第十二卷第二期,8−19 22.Peter H. Dawson, Quadrupole mass spectrometry and its applications, Springer (1995) 46−64 23.J. Throck Watson, O. David Sparkman, Introduction to Mass Spectrometry, John Wiley & Sons (2007) 61−130 24.K. J. McHal, N. Duczak, Applying highly-selective reaction monitoring (H−SRM) for the assay of midazolam and 1-hydroxymidazolam in plasma on the Finnigan TSQ Quantum Ultra, Thermo Scientific Application Note 347 25.Y. Yamagishi, Utility of H-SRM to reduce matrix interference in food residue analysis of pesticides by LC-MS/MS using the TSQ Quantum discovery, Thermo Scientific Application Note 355 26.J. Throck Watson, O. David Sparkman, Introduction to Mass Spectrometry, John Wiley & Sons (2007) 487−503 27.J. B. Fenn, M. Mann, C. K. Meng, S. F. Wong, Mass Spectromety Reviews, 9 (1990) 37−70 28.I. Manisali, D. Y. Chen, B. B. Schneider, Trends in Analytical Chemistry, 25 (2006) 243−256 29.P. Kebarle, U. H. Verkerk, Mass Spectrometry Reviews, 28 (2009) 898–917 30.J. B. Fenn, Journal of the American Society for Mass Spectrometry, 4 (1993) 524−535 31.J. V. Iribarne, B. A. Thomson, The Journal of Chemical Physics, 64 (1976) 2287− 2294 32.B. A. Thomson, J. V. Iribarne, The Journal of Chemical Physics, 71 (1979) 4451−4463 33.L. Tang, P. Kebarle, Analytical Chemistry, 65 (1993) 3654−3668 34.M. Dole, L. L. Make, R. L. Hines, The Journal of Chemistry Physics, 49 (1968) 2240−2249 35.D. Touboul, M. C. Jecklin, R. Zenobi, Rapid Communications in Mass Spectrometry, 22 (2008) 1062−1068 36.J. Throck Watson, O. David Sparkman, Introduction to Mass Spectrometry, John Wiley & Sons (2007) 647−660 37.R. King, R. Bonfiglio, C. Fernandez-Metzler, C. Miller-Stein, T. Olah, Journal of the American Society for Mass Spectrometry, 11 (2000) 942−950 38.H-ESI Probe User Guide, Thermo Fisher Scientific (2009) 1−4 39.A. Y. Yang, L. Sun, D. G. Musson, J. J. Zhao, Journal of Pharmaceutical and Biomedical Analysis, 38 (2005) 521−527 40.R. S. Plumb, R. D. M. Gray, C. M. Jones, Journal of Chromatography B: Biomedical Sciences and Applications, 694 (1997) 123−133 41.X. Ding, B. Chou, R. A. Graham, S. Cheeti, S. Percey, L. C. Matassa, S. A. Reuschel, M. Meng, S. Liu, T. Voelker, B. L. Lum, P. J. Rudewicz, C. E. C. A. Hop, Journal of Chromatography B, 878 (2010) 785−790 42.L. Wu, P. Xie, J. Chen, D. Zhang, G. Liang, Journal of Chromatography A, 1217 (2010) 1455–1462 43.T. Berg, E. Lundanes, A. S. Christophersen, D. H. Strand, Journal of Chromatography B, 877 (2009) 421–432 44.楊雅雯,線上固相萃取結合高效能液相層析檢測水中之苯脲型除草劑,東吳大學化學系碩士學位論文,民國九十四年。 45.U.S. Food and Drug Administration (FDA), Guidance for Industry, http://www.fda.gov/ 46.B. K. Matuszewski, M. L. Constanzer, C. M. Chavez-Eng, Analytical Chemistry, 75 (2003) 3019−3030 47.Walter A. Korfmacher , Using Mass Spectrometry For Drug Metabolism Studies, Taylor and Francis (2005) 103−150 48.C. Chin, Z. P. Zhang, H. T. Karnes, Journal of Pharmaceutical and Biomedical Analysis, 35 (2004) 1149−1167 49.W. Lv, Talanta, 80 (2010) 1406 −1412 50.I. Marchi, V. Viette, F. Badoud, M. Fathi, M. Saugy, S. Rudaz, J.-L. Veuthey, Journal of Chromatography A, 1217 (2010) 4071–4078 51.F. Badoud, E. Grata, L. Perrenoud, M. Saugy, S. Rudaz, J.-L. Veuthey, Journal of Chromatography A, 1217 (2010) 4109–4119 52.Oasis® sample extraction products, Waters (2006) 3−12
摘要: A simple and sensitive method using micro solid-phase extraction (µSPE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination trace amount of tetramethylammonium hydroxide (TMAH) in mouse serum was evaluated in this thesis. The heated electrospray ionization (HESI) with positive mode of triple quadrupole mass spectrometry was used to analyze tetramethylammonium hydroxide. Tetramethylammonium was extracted from serum by µSPE with a mixed-mode cation exchange sorbent and 0.1% trifluoroacetic acid in acetonitrile used as the eluting solvent. The volume of sample used was 15 µL. The linear range of the method was 10-2500 ng/mL with coefficient of determination at 0.9944. The limit of detection and limit of quantitation was 1.9 and 6.3 ng/mL, respectively. The intra-day and inter-day precisions with relative standard deviations were less than 2.5 and 9.8 %, respectively. The recovery of the method was greater than 95.6 %. As the matrix effect was unconsidered from the results of post-column infusion and post-extraction spiked. The feasibility of applying the proposed method to determine tetramethylammonium in serum was examined by analyzing real mouse serum sample of TMAH-exposured. The concentration of tetramethylammonium detected was range from 1.1 µg/mL to 9.1 µg/mL. The results of this study reveal the proposed method offers low ng/mL level sensitivity and high selectivity for determination of tetramethylammonium in mouse serum.
本研究主要是利用微量固相萃取法結合液相層析串聯質譜儀,分析老鼠血清中氫氧化四甲基銨(Tetramethylammonium hydroxide, TMAH)含量。使用混合相陽離子交換(Oasis® MCX)吸附劑之微量固相萃取管萃取血清中四甲基銨,並探討於萃取過程中沖提溶劑種類與沖提溶劑體積對萃取效果之影響,以三段四極矩液相層析串聯質譜儀之加熱式電灑游離法於正離子模式偵測四甲基銨。實驗結果顯示,利用200 µL含0.1%三氟乙酸之乙腈溶液,可得到最佳萃取效率,且僅需15 µL血清樣品即可進行分析。此技術所得相對回收率於不同濃度均高於95.6%,同日(intraday)及異日(interday)之精密度以RSD%表示均低於2.5%及9.8%,於血清中四甲基銨分析線性範圍為10~2500 ng/mL,偵測極限值(LOD)為1.9 ng/mL,定量極限值(LOQ)為6.3 ng/mL。評估基質效應對四甲基銨偵測之影響,以管柱後注入法(post−column infusion)可得基質成分在層析時間中對四甲基銨離子化效率沒有影響;以萃取後添加法(post−extraction spiked)評估基質成分於µSPE-LC-HESI-MS/MS可得基質效應(ME)為95.2%。並將此技術應用於經氫氧化四甲基銨塗覆於皮膚表面之小白鼠血清中,均可有效測得老鼠中四甲基銨濃度為1.1 µg/mL~9.1 µg/mL。
URI: http://hdl.handle.net/11455/16861
其他識別: U0005-1207201123195800
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1207201123195800
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