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Exploration of pathogenicity and DNA variation of Rhizoctonia solani AG-4
random amplified polymorphic DNA (RAPD)
|摘要:||Rhizoctonia solani K(hn is a world-wide soil-borne pathogenic fungus which show tremendous variation in characteristics such as geographic locations, morphology, host specificity and pathogenicity. The relation of pathogenicity and DNA variation of fifteen isolates of Rhizoctonia solani belonging to anastomosis group (AG) 4 from Taiwan and one isolate belonging to HGI were examined. The virulence of each strain was tested in loose leaf lettuce seedlings. The results indicated that eight strains were designated hypervirulent, two strains were middle virulent, four strains were hypovirulent and two strains were non-virulent of the sixteen tested strains. The genetic variation of these strains were evaluated by random amplified polymorphic DNA (RAPD) method with a set of twelve primers. The amplification results revealed scorable polymorphisms among these isolates, and a total of 467 band positions were scored with binary coding and assayed for Numerical Taxonomy and Multivariate Analysis System (NTSYS) software. The results of analysis indicated that the DNA banding patterns of the highly virulent isolates were highly similarity each other. Approximate 650 to 750 base pairs of ITS1-5.8S rDNA ITS2 fragments were amplified by universal primers ITS1 and ITS4 of fungal ribosomal DNA. The fragments were subjected to restriction digestion with recognition endonucleases EcoRI, HaeIII, MboI and different riboprinting patterns were displayed. The virulence of the sixteen isolates can be related with differences in the size of the fragments of ITS1-5.8S rDNA ITS2 and riboprinting patterns.|
|Appears in Collections:||生命科學系所|
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