Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/20693
標題: 立枯絲核菌(Rhizoctonia solani)第四融合群(AG-4)致病性與DNA變異之探討
Exploration of pathogenicity and DNA variation of Rhizoctonia solani AG-4
作者: 黃柏挺
Huang, Bo-Ting
關鍵字: Rhizoctonia solani
立枯絲核菌
random amplified polymorphic DNA (RAPD)
rDNA
riboprinting
pathogenicity
PLANT-SCIENCE
BOTANY
致病性
逢機增幅多型性核酸
核糖指紋
植物科學
植物學
出版社: 植物學研究所
摘要: Rhizoctonia solani K(hn is a world-wide soil-borne pathogenic fungus which show tremendous variation in characteristics such as geographic locations, morphology, host specificity and pathogenicity. The relation of pathogenicity and DNA variation of fifteen isolates of Rhizoctonia solani belonging to anastomosis group (AG) 4 from Taiwan and one isolate belonging to HGI were examined. The virulence of each strain was tested in loose leaf lettuce seedlings. The results indicated that eight strains were designated hypervirulent, two strains were middle virulent, four strains were hypovirulent and two strains were non-virulent of the sixteen tested strains. The genetic variation of these strains were evaluated by random amplified polymorphic DNA (RAPD) method with a set of twelve primers. The amplification results revealed scorable polymorphisms among these isolates, and a total of 467 band positions were scored with binary coding and assayed for Numerical Taxonomy and Multivariate Analysis System (NTSYS) software. The results of analysis indicated that the DNA banding patterns of the highly virulent isolates were highly similarity each other. Approximate 650 to 750 base pairs of ITS1-5.8S rDNA ITS2 fragments were amplified by universal primers ITS1 and ITS4 of fungal ribosomal DNA. The fragments were subjected to restriction digestion with recognition endonucleases EcoRI, HaeIII, MboI and different riboprinting patterns were displayed. The virulence of the sixteen isolates can be related with differences in the size of the fragments of ITS1-5.8S rDNA ITS2 and riboprinting patterns.
URI: http://hdl.handle.net/11455/20693
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.