Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/20693
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dc.contributor.advisor陳昇明zh_TW
dc.contributor.advisorChen, Sheng-Mingen_US
dc.contributor.author黃柏挺zh_TW
dc.contributor.authorHuang, Bo-Tingen_US
dc.date1998zh_TW
dc.date.accessioned2014-06-06T07:14:17Z-
dc.date.available2014-06-06T07:14:17Z-
dc.identifier.urihttp://hdl.handle.net/11455/20693-
dc.description.abstractRhizoctonia solani K(hn is a world-wide soil-borne pathogenic fungus which show tremendous variation in characteristics such as geographic locations, morphology, host specificity and pathogenicity. The relation of pathogenicity and DNA variation of fifteen isolates of Rhizoctonia solani belonging to anastomosis group (AG) 4 from Taiwan and one isolate belonging to HGI were examined. The virulence of each strain was tested in loose leaf lettuce seedlings. The results indicated that eight strains were designated hypervirulent, two strains were middle virulent, four strains were hypovirulent and two strains were non-virulent of the sixteen tested strains. The genetic variation of these strains were evaluated by random amplified polymorphic DNA (RAPD) method with a set of twelve primers. The amplification results revealed scorable polymorphisms among these isolates, and a total of 467 band positions were scored with binary coding and assayed for Numerical Taxonomy and Multivariate Analysis System (NTSYS) software. The results of analysis indicated that the DNA banding patterns of the highly virulent isolates were highly similarity each other. Approximate 650 to 750 base pairs of ITS1-5.8S rDNA ITS2 fragments were amplified by universal primers ITS1 and ITS4 of fungal ribosomal DNA. The fragments were subjected to restriction digestion with recognition endonucleases EcoRI, HaeIII, MboI and different riboprinting patterns were displayed. The virulence of the sixteen isolates can be related with differences in the size of the fragments of ITS1-5.8S rDNA ITS2 and riboprinting patterns.en_US
dc.language.isoen_USzh_TW
dc.publisher植物學研究所zh_TW
dc.subjectRhizoctonia solanien_US
dc.subject立枯絲核菌zh_TW
dc.subjectrandom amplified polymorphic DNA (RAPD)en_US
dc.subjectrDNAen_US
dc.subjectriboprintingen_US
dc.subjectpathogenicityen_US
dc.subjectPLANT-SCIENCEen_US
dc.subjectBOTANYen_US
dc.subject致病性zh_TW
dc.subject逢機增幅多型性核酸zh_TW
dc.subject核糖指紋zh_TW
dc.subject植物科學zh_TW
dc.subject植物學zh_TW
dc.title立枯絲核菌(Rhizoctonia solani)第四融合群(AG-4)致病性與DNA變異之探討zh_TW
dc.titleExploration of pathogenicity and DNA variation of Rhizoctonia solani AG-4en_US
dc.typeThesis and Dissertationzh_TW
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