Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/20940
標題: 立枯絲核菌轉形株之生理特性與交叉保護
The physiological characterizatiions and cross protection of transformant from Rhizoctonia solani Kuehn
作者: 林佩芬
Lin, Pey-Fen
關鍵字: Rhizoctonia solani
立枯絲核菌
physiological
transformant
cross protection
生理特性
轉形株
交叉保護
出版社: 植物學系
摘要: 立枯絲核菌(Rhizoctonia solani Kn)為本省重要的植物病原真菌,能 感染的寄主種類廣泛。R. solani第四融合群(anastomosis group 4,簡稱 AG-4)編號為CHU341之強致病株具有一約2.6 Kb之線形質體;編號CHU344 之弱致病株則不具有線形質體;而R. solani CHU344+pUCH1-ES是經轉形 作用所得的穩定轉形株。本研究以此三株菌來探討生長條件、致病性與線 形質體的關聯。強致病株CHU341對於不同的碳、氮源之適應良好,而弱致 病株CHU344及轉形株CHU344+pUCH1-ES則對養份的利用性較CHU341為差。 在溫度及酸鹼度方面,皆具有一般立枯絲核菌之生理特性,最適生長於27 ℃及pH 6-7之環境下。且轉形株之生理特性與弱致病性之原始母株CHU344 相似。利用其對營養、溫度、酸鹼度之適應性不同,可加以運用於菌株篩 選及生物防治上。轉形株CHU344+pUCH1-ES對蘿蔔植株的感病性及交叉保 護皆介於CHU341及CHU344菌株之間。且蘿蔔植株經轉形株CHU344+pUCH1- ES誘導的幾丁質酵素活性亦呈類似的變化。因此推測線形質體可能與致病 性及幾丁質酵素有關。
In Taiwan, Rhizoctonia solani Kuehnn is an important plant pathogenic fungus. It possesses a wide host range. A strong pathogenic strain CHU341 which belongs to anastomosis group 4 (AG-4) carries a linear plasmid about the size of 2.6 Kb. Another strain CHU344 is a weak pathology without linear plasmid. The other stable transformant CHU344+pUCH1-ES was obtained from CHU341 and CHU344. This study analyzed the relationship between R. solai linear plasmid and growth conditions as well as their pathogenecity.The strong pathogenic strain CHU341 is well adapted to carbon source and nitrogen source. The weakly pathogenic strain CHU344 and the transformant CHU344+pUCH1-ES are sensitive of carbon and nitrogen sources. The optimal temperatures and pH-values for the growth of R. solani strains are 27℃ and pH 6-7. The physiological characteristics of transfor-mant are similar to that of the weakly pathogenic strain CHU344. The results of the growth condition tests could be used for the biological control and selection of R. solani strains. The pathogenecity for radish of the transformant was between CHU341 and CHU344, and the radish's chitinase induced by the transformant had similar relation. The above results suggested that the linear plasmid of R. solani may consist a relationship with pathogenecity and the linear plasmid as well as induction of chitinase.
URI: http://hdl.handle.net/11455/20940
Appears in Collections:生命科學系所

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