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Characterization of terminal deficiency associated with the long arm of chromosome 8 in maize
(complete monosomy，簡稱CM)及部分單染體(partial monosomy，簡稱
The r-X1 deficiency in maize was first isolated with X irradiation by L.J.Stadler. It is a submicroscopic, intercalary deficiency located on chromosome 10. It is transmitted to progeny only through the maternal plant. It can induce the formation of monosomic (or CM) and partial monosomic (or PM) progeny as the result of chromosome nondisjunction and breakage that occur during megagametogenesis. The purpose of this study is to map the breakpoints of paitial monosome 8s by using the well-mapped RFLP (restriction fragment length polymorphism) markers, and to use them to reevaluate the accuracy of current RFLP linkage map of the long arm chromosome 8. In this study, three partial monosomic plants were obtained, one of them had been lost. The remaining two in addition to seven PMs obtained earlier were used for RFLP analysis. The result shows that the breakpoints of these nine PMs are not located at the same chromosome location. Five of them are located in the 103A - umc12 (umc93) region, and three others are located in the umc12 (umc93) - umc30 rejion. The last one is located in the region between umc30 and j1 locus. The physical map of RFLP markers on the long arm of chromosome 8 as constructed by using these nine partial monosomes is consistent with the conventional linkage map: no rearranged gene order is observed. This study also map centromere of chromosome 8 to the chromosome region close to umc 103A.
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