Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/21189
標題: 實體定位玉米第十對染色體長臂之RFLP分子標誌
Physical mapping RFLP markers on the long arm of chromosome 10 in maize
作者: 彭淑芬
Perng, Shu-Fen
關鍵字: r-X1缺失
單染體
雙染體
末端缺失
部分單染體
實體圖
r-X1 deletion
monosome
trisome
terminal deficiency
partial
出版社: 分子生物研究所
摘要: 玉米第十條染色體長臂上帶有一小段缺失,稱之為r-X1缺失.該缺失包含R 與Lc基因,及其附近區域.帶有此缺失的第十對染色體,不能經由父本傳遞 ,僅能由母本遺傳.它會引起不同條染色體形成單染體(monosome)及三染 體(trisome).此外,也會引起低頻率母本染色體斷裂,其中不帶有染色體中 節(centromere)的部分,會從細胞中消失,而帶有中節的染色體則保留在 細胞中,成為染色體未端缺失(terminal deficiency),也稱為部分單染 體(partial monosome;或簡稱PM)。本論文之目的是以r-X1缺失誘導產第 十對染色體長臂(10L)之PM,並用來實體標定(physical mapping)該染色 體臂的RFLP標誌(RFLP markers)。本實驗中,PM是以r-X1/R-r(W22)為母 本與sr2隱性植株雜交的後代,篩選產生。該雜交產生2941顆無色r-X1種 子,發芽後(存活2054顆種子),從中再以根尖及花粉母細胞分析,鑑別5 株為PM(及16株CM)。這五個PM的斷裂位置,分佈在10L上的兩個不同區域 。RFLP分析顯示,其中4個PM斷裂在sr2附近,介於uaz318和bnl10.13( bnl17.02)標誌之間。在標定PM的過程中,本實驗也同時建立10L的實體 圖(physical map)。該圖與現有的連鎖圖比較,有兩點不同:第一 , bnl10.13在連鎖圖上位於 csu06B與uaz251C之間,而在實體圖上,卻落在 uaz318和sr2之間,兩者相差超過40個連鎖單位(cM);第二,bnl17.02在 連鎖圖上是介於uaz251C與 bnl7.49之間,而在實體圖上,轉移到uaz318 與sr2之間。換言之,把該標誌朝sr2方向變動了約15cM。這種差異,充分 顯示PM的實體定位功能。
The r-X1 deletion is a small deficiency located on the long arm of chromosome 10. When crossed as female, it induces the formation of monosome and trisome, in addition to a low frequency of chromosome breakage. The resulting chromosome fragment not carrying the centromere is lost, while the centromere-carrying fragment is retained in the cell to become a terminal deficiency designated as partial monosome(or PM). The objective of this thesis is to use the r-X1 deletion for the indution of PMs associated with the long arm of chromosome 10(or 10L)and to use them to do the physical mapping of RFLP markers on 10L. Partial monosomes were produced from the cross of r-X1/R-r(W22) as pistillate flower with sr2 pollen. From 2941 r-X1-carrying F1 progeny, 30 sr2 plants were isolated, five of which were identified as PMs by the subsequent root-tip and meiotic analysis. The breakpoints of these PMs are distributed in two 10L regions: one is in the csu06B-- centromere region(close to the centromere) and the other, in the uaz318--bnl10.13 region(near the sr2 locus). These PMs were used to construct the physical map of 10L. Two RFLP markers, bnl10.13 and bnl17.02, on the map have a position different from that on the linkage map. The bnl10.13 is located in the csu6B--uaz251C region on the physical map but in uaz318--sr2 region on the linkage map, representing a change of more than 40 map units. The bnl17.02 is located in the uaz251C--bnl7.49 region on the linkage map but in the uaz318--sr2 region on the physical map. This difference moves bnl17.02 at least 15 map units away from the centromere. This result demonstrates the efficiency of PM for physical mapping.
URI: http://hdl.handle.net/11455/21189
Appears in Collections:分子生物學研究所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.