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標題: 應用ISSR研究台灣水柳與水社柳之遺傳變異
Genetic variation of Salix warburgii and S. kusanoi based on ISSR markers
作者: 蔡承憲
Tsai, Cheng-Shien
關鍵字: Salix warburgii
Salix kusanoi
Genetic variation
出版社: 植物學系
摘要: 水柳與水社柳為台灣兩種濕生的柳屬植物,分布於海拔1500公尺以下的濕地、湖泊及河流旁,其中水社柳為稀有瀕臨滅絕的植物而水柳則較為廣泛。本研究共採集水柳12個族群144個樣本,水社柳3個族群62個樣本,以ISSR研究其遺傳變異。共測試了125個引子,並選取條帶清晰且具多型性及再現性的引子,共計水柳18個引子113個多型性條帶,水社柳15個引子50個多型性條帶。POP- GENE分析得到遺傳分化係數(Gst),水柳與水社柳分別為0.182與0.494,AMOVA分析得到水柳地理區域間(北、中、南、東四區)變方成分佔總變方成分的9.99% (P<0.001),地理區域內族群間變方成分佔總變方成分的23.23% (P<0.001),族群內個體間變方成分佔總變方成分的66.78% (P<0.001),水社柳族群間變方成分佔總變方成分的59.70% (P<0.001),族群內個體間變方成分佔總變方成分的40.30% (P<0.001)。UPGMA歸群及主座標分析結果顯示水柳族群中,池上與左營族群的差異較大,可能是雜交的原因;而水社柳各族群間的分化程度較明顯,可能是因為長時間的隔離及基因流受到限制的關係。
Salix warburgii and Salix kusanoi (Salicaceae) are two hydrophilic species of the genus Salix in Taiwan. They are generally found in lake and river bank at the altitude below 1500 meters. S. kusanoi is a rare and en-dangerous species while S. warburgii has a wider distribution. In the present study, 144 individual samples from 12 populations of S. warburgii and 62 individuals from three populations of S. kusanoi were studied using ISSR markers. One-hundred and twenty-four ISSR primers were screened and primers that produced reproducible polymorphic bands were used. Eighteen primers were used for S. warburgii and 113 polymorphic bands were recorded. Fifty polymorphic bands were recorded in S. kusanoi using 15 primers. By employing POPGENE software, the gene diversity coefficient (Gst) of S. warburgii and S. kusanoi were 0.182 and 0.494. respectively. The analysis of molecular variance (AMOVA) revealed that the variance components among regions (north, central, south and east regions), among populations within regions, and among individuals within populations were 9.99% (p<0.001), 23.23% (p<0.001) and 66.78% (p<0.001) of the total variance, respectively in S. warburgii. In S. kusanoi, the variance components among populations and among individuals within populations were 59.70% (p<0.001) and 40.30% (p,0.001) of the total variance, respectively. The results of UPGMA cluster analysis and principal coordinate analysis indicated that, within S. warburgii, Tsusang and Tsuoyin populations were the two more isolated populations that may resulted from interspecies hybridization. S. kusano had greater diversity among populations which may resulted from longtime geographic isolation and limited gene flow among populations.
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