Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/21519
DC FieldValueLanguage
dc.contributor.advisor陳昇明zh_TW
dc.contributor.advisorChen, Sheng-Mingen_US
dc.contributor.author陳奇良zh_TW
dc.contributor.authorChen, Qi-Liangen_US
dc.date1990zh_TW
dc.date.accessioned2014-06-06T07:15:57Z-
dc.date.available2014-06-06T07:15:57Z-
dc.identifier.urihttp://hdl.handle.net/11455/21519-
dc.description.abstract枯草桿菌 ( Bacillus subtilis )F29-3 能產生一種抗絲狀真菌的抗生素--fengymy- cin (fengycin) 。 本論文是利用轉位子 (transposon)Tn917, 作為快速選殖fengy- mycin合成基因的工具。 首先, 將帶有Tn917 的質體pTV1以原生質體轉形法送入枯草桿菌F29-3 中。並以紅黴 素誘使Tn917 發生轉位作用。篩選得十株不產生fengymycin的突變株。再將帶有pBR3 22-Tn917融合的線狀質體pTV20 轉形到已除去pTV1的四株突變株中, 並發生同源置換 重組作用 (homologously replacemental recombination),而將抗氯黴素基因及pBR3 22的DNA 序列由pTV20 置換到突變株的Tn917 中。篩選抗氯黴素的轉形株。 將轉形株 FEX11, FEX31, FEX51, 及FEX71 的染色體分別以ECORI(或SphI) 切割, 這 些片段再自身圈接 (self-ligation), 並轉形到 E. coli HB101中, 篩選抗安黴素 ( 或抗安黴素加氯黴素) 的 E. coli HB101菌株。經DNA 雜交試驗 (DNA hybridizati- on ), 證實, 送入 E. coli HB101中的轉移質體pBTE71, pBTS51, 及pBTS71均含有pB R322的DNA 序列, Tn917 的右臂 (distal arm ), 及枯草桿菌F29-3 的染色體片段。 如此, 篩選得fengymycin的突變基因。zh_TW
dc.language.isoen_USzh_TW
dc.publisher植物學研究所zh_TW
dc.subjectBACILLUS-SUBTILISen_US
dc.subject枯草桿菌zh_TW
dc.subjectTRANSPOSONen_US
dc.subjectHMOLOGOUSLY-REPLACEMENTAL-RECOen_US
dc.subjectPLANT-SCIENCEen_US
dc.subjectBOTANYen_US
dc.subjectBacillus subtilisen_US
dc.subjectFengycinen_US
dc.subjectAntifungal antibioticen_US
dc.subjectLipopeptide antibioticen_US
dc.subjectF29-3豐原素基因zh_TW
dc.subject轉位子zh_TW
dc.subject同源置換重組作用zh_TW
dc.subject抗氯黴素基因zh_TW
dc.subjectPBR 322的DNA序列zh_TW
dc.subject植物科學zh_TW
dc.subject植物學zh_TW
dc.subject枯草桿菌zh_TW
dc.subject中豐原素zh_TW
dc.subject合成基因zh_TW
dc.subject枯草桿菌zh_TW
dc.subject豐原素zh_TW
dc.subject抗真菌之抗生素zh_TW
dc.subject脂胜呔抗生素zh_TW
dc.title枯草桿菌( Bacillus subtilis )F29-3 豐原素基因的研究zh_TW
dc.titleANALYSIS OF THE GENES ENCODING THE BIOSYNTHESIS OF FENGYCIN IN BACILLUS SUBTILIS F29-3en_US
dc.typeThesis and Dissertationzh_TW
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