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標題: 應用ISSR研究刺柏之族群遺傳變異
Genetic Variation of Juniperus formosana Hayata Based on ISSR Markers
作者: 蔡依錡
Tsai, Yi-Chi
關鍵字: Juniperus formosana
genetic variation
出版社: 生命科學系
摘要: 刺柏(Juniperus formosana Hayata)為台灣原生柏屬植物之一, 主要生長於海拔2500公尺以上之高山草原或岩石裸露地;僅少數生長 於中低海拔地區,為台灣高山植物群落社會初生或次生演替的代表灌 叢樹種之一。本實驗以10個ISSR引子研究刺柏的8個族群共117個個體 ,總共得到126條條帶,其中有80條為多型性條帶;佔63.49%。AMOVA 分析結果顯示,地區間變方成分佔5.26%(P<0.001),地區內族群間 變方成分佔18.96%(P<0.001),族群內個體間變方成分佔75.78%(P <0.001)。POPGENE分析結果顯示,各族群內之基因歧異度(H)介於0.1593-0.2458之間,總Nei's基因歧異度(Ht)為0.3536,遺傳分化 係數(Gst)為0.2177,基因流(Nm)為1.797。由上述結果看來,族 群間的分化程度則高於一般針葉樹平均。比較各族群發現,花蓮綠水 族群與其他族群有明顯的分化。而同地區內之族群方面,向陽山與玉 山、關山族群間亦有明顯分化,另外雪山族群與同山系的大霸尖山族 群也有較高的遺傳變異存在。
Juniperus formosana is generally distributed in mountain grasslands and rocky habitats above 2,500m in altitude in Taiwan. Few populations also occur in lower altitude on the east side of the island. It is a representative first or second successive tree species of the high mountain plant community. In the present study, 117 individuals from 8 populations were studied for ISSR variation. One hundred and twenty-six bands were obtained using 10 primers. Eighty bands (63.49%) were polymorphic bands. AMOVA analysis revealed that the perceontages of variance components attributable to variation among regions, among population within regions, and among individuals within populations were 5.26% (P<0.001), 18.96% (P<0.001), and 75.78% (P<0.001), respectively. POPGENE analysis revealed that the total gene diversity (Ht) of the species was 0.3536 and the gene diversity of populations ranged from 0.1593 to 0.2458. The population genetic diversity index (Gst) was 0.2177 while the gene flow index (Nm) was 1.797. The result indicated that the population differentiation was higher than the average of conifer species. There is a significant genetic difference between Liu-Shue population of Hualien County and the rest population. Sian-Yang population is significantly different from Yushan and Guanshan population of the same southern region. Shieshan population is significantly different from Dabagienshan population of the same moutain range.
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