Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/21852
標題: Development of Transgenic Rice for Producing Transglutaminase
開發基因轉殖水稻生產轉榖氨醯胺酵素之研究
作者: 劉穎
Liu, Yin
關鍵字: Transgenic Rice
轉殖水稻
Transglutaminase
轉榖氨醯胺酵素
出版社: 分子生物學研究所
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Microbial transglutaminase–a review of its production and application in food processing. Appl. Microbial. Biotechnol. 44 : 277-282.
摘要: 摘 要 水稻是台灣最主要農作產業,提供國產稻米附加價值是台灣水稻產業的一大重要課題。如果能以水稻作為生物反應器來生產高價值的酵素或蛋白質,對提高稻米的附加價值是一非常可行方法。轉榖氨醯胺酵素 (transglutaminase, TGA)會使蛋白濃縮液形成膠體化,因此在食品加工上有相當高的應用價值及潛力;例如:在漢堡、肉丸、魚漿、豆腐、植物蛋白粉末等可以改善其彈性、質地、口感、風味,並可增加儲存壽命。中興大學分子生物研究所楊明德博士實驗室已由放線菌Streptomyces kentuckense中篩選出轉榖氨醯胺酵素基因 (tga)。本研究乃嘗試將tga基因分別黏接至水稻種子特有之啟動子鹽溶性球蛋白基因 (globulin) 及油膜蛋白基因 (oleosin) 之啟動子與 CaMV35S及rbcS啟動子上,利用農桿菌基因轉移的方式,轉殖到''台農67號''水稻中。本研究之目的為建立以轉殖水稻為生物反應器之系統,並探討利用水稻生產轉榖氨醯胺酵素之可行性。 本研究已完成將放線菌Streptomyces kentuckense中篩選出的轉榖氨醯胺酵素基因(tga) 構築到植物轉殖載體,利用農桿菌轉移法將pBI121-S.K-tga (CaMV35S啟動子)、pBI131-S.K-tga (rbcS啟動子)、pGlo-S.K-tga (globulin啟動子) 、pOle-S.K-tga (oleosin啟動子) 等質體之tga基因轉移到''台農67號''水稻癒傷組織,並誘導再生成植株。四種組合之轉殖均已獲得再生植株。轉殖植株以PCR、南方墨點、RT-RCR及西方墨點雜交分析,其結果顯示tga基因已存在於轉殖之水稻植株基因組中,且可正確表現tga mRNA,並表現出TGA酵素蛋白。
Abstract Rice is the major agricultural industry in Taiwan. The major course to developing and continuing the rice agriculture in Taiwan is to providing the additive values of domestic rice. Certainly, establishing the rice bioreactor system to producing high-value enzymes and proteins is a feasible way to advance the additive values of rice. Transglutaminase (TGA) is an enzyme capable of stabilizing protein assemblies by gamma-glutamyl-epsilon-lysine crosslinks. The specific function of transglutaminase allows their biotechnological application in the foodstuffs industry: fish products (surimi), processed meat and sausages, chesses and yoghurt, ice creams, gelatines, chocolate etc. because food texture, firmness, elasticity, or fat and salt content can be modified. Several transglutaminase genes (tga) had been cloned from Streptomyces spp. by Dr. Ming-Te Young's laboratory in Institute of Molecular Biology, National Chung Hsing University. The purpose of this study is to explore the possibility for overproducing the TGA in rice via transformed tga gene. We attempt to establish the bioreactor system by using the rice as a model plant to producing TGA. In this study, the tga gene isolated from Streptomyces kentuckense was constructed into plant transformation vectors driven by CaMV 35S, rbcS, oleosin, and globulin promoter. The constructed genes were transferred into TN67 rice callus via Agrobacterium-mediated transformation. The regenerated plants were primary selected by G418. The results of PCR, Southern, RT-PCR and Western hybridization analysis indicated that the tga gene was present in the genome of transformed rice, and expressed tga mRNA and TGA protein with enzyme activity.
URI: http://hdl.handle.net/11455/21852
其他識別: U0005-2308200712245900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2308200712245900
Appears in Collections:分子生物學研究所

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