Please use this identifier to cite or link to this item:
The effect of miR160a and miR167b on rice growth and development
|引用:||Abel S, Theologis A (1996) Early genes and auxin action. Plant Physiol 111: 9-17 Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116: 281-297 Chen X (2004) A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science 303: 2022-2025 Dugas DV, Bartel B (2008) Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases. Plant Mol Biol 67: 403-417 Dunoyer P, Himber C, Voinnet O (2005) DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal. Nat Genet 37: 1356-1360 Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW (2005) AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development 132: 4563-4574 Gandikota M, Birkenbihl RP, Hohmann S, Cardon GH, Saedler H, Huijser P (2007) The miRNA156/157 recognition element in the 3'' UTR of the Arabidopsis SBP box gene SPL3 prevents early flowering by translational inhibition in seedlings. Plant J 49: 683-693 Goetz M, Vivian-Smith A, Johnson SD, Koltunow AM (2006) AUXIN RESPONSE FACTOR8 is a negative regulator of fruit initiation in Arabidopsis. Plant Cell 18: 1873-1886 Guilfoyle TJ, Hagen G (2001) Auxin response factors. J Plant Growth Reg 10: 281-291 Guilfoyle TJ, Hagen G (2007) Auxin response factors. Curr Opin Plant Biol 10: 453-460 Guilfoyle T, Hagen G, Ulmasov T, Murfett J (1998) How does auxin turn on genes? Plant Physiol. 118: 341-347 Hardtke CS, Berleth T (1998) The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. Embo J 17: 1405-1411 Jones-Rhoades MW, Bartel DP (2004) Computational identification of plant microRNAs and their targets including a stress-induced miRNA. Mol. Cell 14: 787-799 Jung HJ, Kang H (2007) Expression and functional analyses of microRNA417 in Arabidopsis thaliana under stress conditions. Plant Physiol Biochem 45: 805-811 Kurihara Y, Watanabe Y (2004) Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions. Proc Natl Acad Sci U S A 101: 12753-12758 Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75: 843-854 Li J, Dai X, Zhao Y (2006) A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis. Plant Physiol. 140: 899-908 Li J, Yang Z, Yu B, Liu J, Chen X (2005) Methylation protects miRNAs and siRNAs from a 3''-end uridylation activity in Arabidopsis. Curr Biol 15: 1501-1507 Liang X, Abel S, Keller JA, Shen NF, Theologis A (1992) The 1-aminocyclopropane-1-carboxylate synthase gene family of Arabidopsis thaliana. Proc Natl Acad Sci U S A 89: 11046-11050 Llave C, Xie Z, Kasschau KD, Carrington JC (2002) Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science 297: 2053-2056 Nagpal P, Ellis CM, Weber H, Ploense SE, Barkawi LS, Guilfoyle TJ, Hagen G, Alonso JM, Cohen JD, Farmer EE, Ecker JR, Reed JW (2005) Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation. Development 132: 4107-4118 Nakagawa N, Mori H, Yamazaki K, Imaseki H (1991) Cloning of a complementary DNA for auxin-Induced 1-Aminocyclopropane-1-carboxylate synthase and differential expression of the gene by auxin and wounding. Plant Cell Physiol. 32: 1153-1163 Okushima Y, Overvoorde PJ, Arima K, Alonso JM, Chan A, Chang C, Ecker JR, Hughes B, Lui A, Nguyen D, Onodera C, Quach H, Smith A, Yu G, Theologis A (2005) Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19. Plant Cell 17: 444-463 Orban TI, Izaurralde E (2005) Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome. Rna 11: 459-469 Park W, Li J, Song R, Messing J, Chen X (2002) CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. Curr Biol 12: 1484-1495 Pekker I, Alvarez JP, Eshed Y (2005) Auxin response factors mediate Arabidopsis organ asymmetry via modulation of KANADI activity. Plant Cell 17: 2899-2910 Reyes JL, Chua NH (2007) ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant J 49: 592-606 Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP (2002) Prediction of plant microRNA targets. Cell 110: 513-520 Ru P, Xu L, Ma H, Huang H (2006) Plant fertility defects induced by the enhanced expression of microRNA167. Cell Res 16: 457-465 Sasaki T, Burr B (2000) International Rice Genome Sequencing Project: the effort to completely sequence the rice genome. Curr Opin Plant Biol 3: 138-141 Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ (2006) The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs. Development 133: 251-261 Schwarz S, Grande AV, Bujdoso N, Saedler H, Huijser P (2008) The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in Arabidopsis. Plant Mol Biol 67: 183-195 Sessions A, Nemhauser JL, McColl A, Roe JL, Feldmann KA, Zambryski PC (1997) ETTIN patterns the Arabidopsis floral meristem and reproductive organs. Development 124: 4481-4491 Song JJ, Liu J, Tolia NH, Schneiderman J, Smith SK, Martienssen RA, Hannon GJ, Joshua-Tor L (2003) The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol 10: 1026-1032 Song JJ, Smith SK, Hannon GJ, Joshua-Tor L (2004) Crystal structure of argonaute and its implications for RISC slicer activity. Science 305: 1434-1437 Tian CE, Muto H, Higuchi K, Matamura T, Tatematsu K, Koshiba T, Yamamoto KT (2004) Disruption and overexpression of auxin response factor 8 gene of Arabidopsis affect hypocotyl elongation and root growth habit, indicating its possible involvement in auxin homeostasis in light condition. Plant J 40: 333-343 Tiwari SB, Hagen G, Guilfoyle T (2003) The roles of auxin response factor domains in auxin-responsive transcription. Plant Cell 15: 533-543 Ulmasov T, Hagen G, Guilfoyle TJ (1997a) ARF1, a transcription factor that binds to auxin response elements. Science 276: 1865-1868 Ulmasov T, Hagen G, Guilfoyle TJ (1999) Dimerization and DNA binding of auxin response factors. Plant J 19: 309-319 Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ (1997b) Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9: 1963-1971 Vaucheret H (2006) Post-transcriptional small RNA pathways in plants: mechanisms and regulations. Genes Dev. 20: 759-771 Wang D, Pei K, Fu Y, Sun Z, Li S, Liu H, Tang K, Han B, Tao Y (2007) Genome-wide analysis of the auxin response factors (ARF) gene family in rice (Oryza sativa). Gene 394: 13-24 Wang JW, Wang LJ, Mao YB, Cai WJ, Xue HW, Chen XY (2005) Control of root cap formation by MicroRNA-targeted auxin response factors in Arabidopsis. Plant Cell 17: 2204-2216 Wightman B, Burglin TR, Gatto J, Arasu P, Ruvkun G (1991) Negative regulatory sequences in the lin-14 3''-untranslated region are necessary to generate a temporal switch during Caenorhabditis elegans development. Genes Dev 5: 1813-1824 Wightman B, Ha I, Ruvkun G (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75: 855-862 Wilmoth JC, Wang S, Tiwari SB, Joshi AD, Hagen G, Guilfoyle TJ, Alonso JM, Ecker JR, Reed JW (2005) NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J 43: 118-130 Wu MF, Tian Q, Reed JW (2006) Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development 133: 4211-4218 Xie Z, Johansen LK, Gustafson AM, Kasschau KD, Lellis AD, Zilberman D, Jacobsen SE, Carrington JC (2004) Genetic and functional diversification of small RNA pathways in plants. PLoS Biol 2: E104 Zamore PD, Haley B (2005) Ribo-gnome: the big world of small RNAs. Science 309: 1519-1524 Zeng Y, Yi R, Cullen BR (2003) MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc Natl Acad Sci U S A 100: 9779-9784 Zhou GK, Kubo M, Zhong R, Demura T, Ye ZH (2007) Overexpression of miR165 affects apical meristem formation, organ polarity establishment and vascular development in Arabidopsis. Plant Cell Physiol 48: 391-404|
|摘要:||microRNA為一群小片段且不會轉譯出蛋白的RNA，其大小約為19-25核醣核酸，microRNA主要能抑制並調節其目標基因的表現，在植物的研究中發現，microRNA能調節細胞分化生長、開花時間、荷爾蒙與逆境的反應等等。除此之外，也有研究指出某些microRNA具有組織特異性，為了了解水稻中microRNAs的表現位置與特性，前人利用microarray技術對水稻microRNA進行初步檢測，在眾多水稻microRNA中，發現miR160a主要表現在水稻孕穗時期的穗組織中，當水稻抽穗後表現就下降且消失。經序列分析後發現，OsARF18與OsARF22可能為miR160a所調控的目標基因，miR160a分別能互補在兩基因第二個exon上。real-time RT-PCR結果顯示OsARF18與OsARF22表現於所有偵測的組織中，但是在miR160a表現多的孕穗時期穗組織中表現量較低。為了瞭解miR160a在水稻的生理與生長所扮演的角色，利用農桿菌轉殖技術得到含有大量表現miR160a的水稻轉殖株，經real-time RT-PCR結果可知OsARF18與OsARF22表現受到抑制，除此之外也利用RNA Ligase-Mediated 5’RACE（Rapid Amplification of 5’ cDNA Ends）確定OsARF18與OsARF22的mRNA會受到miR160a切割，因此證明了其為miR160a所調控的基因。進一步觀察發現轉殖株並無明顯的生長異常，除了在發芽的時期有輕微發芽延遲的現象，在植株進入種子黃熟時期，也發現轉殖株的葉片較早黃化。第二個研究的microRNA為miR167b，在先前microarray的結果發現，其主要表現在第100天劍葉與下位葉中，同樣的經由序列比對分析，其可能的目標基因為OsARF25，在大量表現miR167b的轉殖株中雖然可以看出OsARF25的mRNA受到抑制，卻無法利用RLM 5’RACE直接的証明OsARF25為miR167b的目標基因，且受到miR167b的調控。在這些轉殖株中，大量表現miR167b造成植株生長異常的現象發生，其植株較矮，分蘗少且呈現扇形狀的生長。本研究已經初步證明了microRNAs所調控的目標基因，也發現大量表現microRNAs對植物所造成的影響，但是microRNA影響植物生長與發育的詳細情形，未來必須做更深入的探討。|
MicroRNAs (miRNAs), a group of small noncoding RNAs, are approximately 19-25 nucleotides in length. These miRNAs down-regulate their target genes at the post-transcriptional level. In plants, miRNAs regulate their target genes expression mostly by guiding mRNA cleavage. Many reports indicate that miRNAs are involved in cell differentiation, cell growth, organogenesis, flowering time, hormone response, stress response, antibacterial resistance in plants. Recent studies demonstrated that expressions of some miRNAs are tissue-specific suggesting that those miRNAs play important roles in plant development and growth. In order to explore tissue-specific miRNA expression profile in rice, we have established a rice miRNA microarray. Our preliminary microarray results showed that expression of miR160a is mostly in booting panicle. From sequences analysis, we identified that the second exon of rice OsARF18 and OsARF22 genes contains complementary sequence of miR160a and predicted that it can be regulated by miR160a. Real-time RT-PCR analysis revealed high expression of OsARF18 and OsARF22 in all tested tissues except booting panicle which showed high level of miR160a. To explore the biological function of miR160a, a construct containing precursor of miR160a was obtained and used for transforming rice. Transgenic rice over-expressing miR160a could suppress the expressions of OsARF18 and OsARF22 genes. The RLM-5'RACE assay demonstrated that OsARF18 and OsARF22 genes are the targets of miR160a and their targeted-cleavage sites were identified. However, all transgenic rice did not reveal any obvious changed in phenotype except a slightly delay of seed germination and earlier leaves yellowing during the late ripening stage. A second miRNA, miR167b that used in this study was detected mainly in flag leaf and in the leaf below the flag leaf by microarray assay. Similar transgenic approach to that of miR160a was applied for miR167b, and results showed that the expression of OsARF25, a gene with a complementary sequence to the mature miR167b, was suppressed in transgenic plants expressing miR167b. While the targeted-cleavage site of OsARF25 by miR167b can not be identified, these transgenic rice plants revealed dwarf and less tillers. However the mechanisms of miRNAs involved in regulating plant growth and development required further studies.
|Appears in Collections:||分子生物學研究所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.