Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22054
標題: 包氏不動桿菌的分子特性與流行病學之研究
Molecular Characterization and Epidemiological Study of Clinical Acinetobacter baumannii
作者: 陳昶華
Chen, Chang-Hua
關鍵字: Acinetobacter baumannii
包氏不動桿菌
IntI1 gene
bla Tem—1 gene
genotype
phenotype
IntI1
bla Tem—1
表現型
基因型
出版社: 生命科學院碩士在職專班
摘要: 近年彰化基督教醫院出現具有imipenem抗藥性的包氏不動桿菌(Acinetobacter baumannii) 後,包氏不動桿菌就被引起注意。加上抗生素抗藥性的問題日益嚴重,因此本研究主要目的首要建立彰化基督教醫院等中部地區包氏不動桿菌的分子流性病學資料庫,其次分析表現型與基因型的相關性,及探討抗藥性基因(bla Tem—1與 IntI1)的水平傳播功能。以民國90年四月1日到民國91年三月31日為止,在彰化基督教醫院分離出之401 株包氏不動桿菌為材料,以PCR方法進行抗藥性基因( bla Tem—1與 IntI1基因)之研究。依照finite mixture方法,將菌株的抗生素抗藥性表現型分為三組(clusters)32群(subgroup)。帶有bla Tem-1 基因者佔31.2 % (10/32) ,與帶有IntI1 基因者佔81.3% (26/32)。其次吾人也發現E. coli (AF427133)與 A. baumannii CH 7的bla Tem-1 基因有100%相似性(1044/1044)。根據彰基對此菌株的SXT 藥物敏感實驗結果,發現81.3% (26/32)帶有IntI1 基因,其中 84.6%( 22/26) IntI1 陽性菌株中會對SXT有抗藥性,也發現其中80.8% (21/26)IntI1 陽性菌株中會對gentamicin有抗藥性。根據彰基對此菌株的CAZ 藥物敏感實驗結果,發現31.2% (10/32)帶有bla Tem-1 基因,其中 100%( 10/10) bla Tem-1陽性菌株會對CAZ有抗藥性。本研究的結論為包氏不動桿菌的IntI1 基因可能跟 SXT 抗藥性有關係,與bla Tem-1基因可能跟 CAZ 抗藥性有關係。關於遺傳情報水平傳播的部分有待進一步的研究。
The Acinetobacter baumannii was noticed since imipenem-resistant had emerged recently at Changhua Christian Hospital, Changhua, Taiwan. Because of the growing importance of antibiotic resistant strains of A. baumannii, this study firstly established the native clinical epidemiological database of A. baumannii strains in the central Taiwan area, secondarily analyzed the relationship between genotype (bla Tem—1, and IntI1 gene) and the antibiotic resistance phenotype, and studied the horizontal gene transfer of the bla Tem—1. A cross-sectional, prospective study was conducted at Changhua Christian Hospital during April 1, 2001, and March 31, 2002. A total of 401 clinical A. baumannii strains were collected. The PCR method was used to detect the target resistant genes (bla Tem-1 gene , IntI1 gene). We divided the 401 clinical strains into the 3 clusters and 32 subgroups according to the antibiotics resistant patterns by the finite mixture method. 31.2 % (10/32) A. baumannii possessed the bla Tem-1 gene, while 81.3% (26/32) possessed the IntI1 genes. The DNA sequence of bla tem gene was identical (1044/1044 bp) between the E. coli (AF427133) and the A. baumannii CH 7. According to the SXT susceptibility tests and PCR analysis of the Changhua Christian Hospital, 81.3% (26/32) of clinical strains carried the IntI1 gene, and 84.6%( 22/26) IntI1 positive strains were resistant to SXT. 80.8% (21/26) in IntI1 positive strains were resistant to gentamicin and 16.7%(1/6) in IntI1 negative strains were resistant to gentamicin. The IntI1 gene could result in the SXT and gentamicin resistance. Furthermore, CAZ susceptibility tests and PCR analysis showed that 31.2% (10/32) of clinical strains carried the bla Tem-1 gene, and 100%( 10/10) bla Tem-1 positive strains were CAZ resistant. We concluded that the IntI1 genes could relate to the SXT resistance, and the bla Tem—1 gene could relate to the CAZ resistance. The horizontal gene transfer of the bla Tem—1 gene needed further experiments
URI: http://hdl.handle.net/11455/22054
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