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|標題:||立枯絲核菌(Rhizoctonia solani Kuhn)AG-4 融合群品系間之生理特性及交叉保護作用|
The physiological characteristics and cross protection between strains of Rhizoctonia solani Kuhn AG-4
境, 氮源中以甘氨酸(Glycine)對生長較不適宜; 而無質體之菌株適合中
、弱酸性生長環境, 以酥氨酸 (Threonine) 為氮源對生長較不適宜。感
Rhizotonia solani is a pathological fungi in the soil. It often causes serious diseases of plants. There are thirteen kinds of Rhizotonia solani strains Kuhn AG-4 in Taiwan. Among them, CHU344 and CHU345 are the only two strains without plasmids. We analyzed the relationship between plasmids and R.solani's necessary growth conditions, pathology and cross protection. In our study of the nutrition ultilization,the thirteen strains can grow using different carbon sources. The eleven which contain plasmids grow favorably in alkaline. They can't grow well when we use glycine as the only nitrogen source.And strains without plas- mids are apt to grow up in neutral conditions but do not grow well using threonine as the only nitrogen source.In the infection tests, learned that CHU344 was the strain with a weak pathology, CHU341 had a stronger pathology, and the others had the strongest pathology. CHU341, CHU344,CHU345,CHU347 and CHU350 showed a cross protection against the strains of strong pathology. Therefore , there may not be a direct relation between plasmids and R. solani's growth conditions and pathology. From the extract of the strain with a cross protection function. We speculate that chiti- nase, which is induced by infection, could be involved in the effect of cross protection. If the plasmid isn' t concerned with pathology, then the plasmid can be used as a vector in fungal genetic techology. Then the re- sult of growth condition tests and cross protection can be used for biological control.
|Appears in Collections:||生命科學系所|
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