請用此 Handle URI 來引用此文件：
The chlorophyll fluorescence and leaf reflectance spectra characteristics among Sweet potato(Ipomoea batatas (L.) Lam.) genotypes with various leaf colors
photochemical electron transport
photochemical reflectance index
|引用:||石峰吉。2005。不同葉色芥藍菜的葉綠素螢光及葉片反射光譜特性。國立中興大學生命科學系碩士論文。 高世昌。2006。不同光照下彩葉草的葉綠素螢光及反射光譜特性。國立中興大學生命科學系碩士論文。 許明晃。2003。甘藷葉片色素含量與反射光譜關係之研究。國立台灣大學農藝系博士論文。 黃秀鳳、黃文達、許明晃、楊志維、趙壁玉、張新軒、蔡養正、楊棋明。2004。三種不同顏色甘藷葉片葉綠素合成能力之分析。作物、環境與生物資訊1 : 47-54。 賴美鳳。2003。光度與溫度對葉綠素螢光特性之影響。國立中興大學生命科學系碩士論文。 Adams, III W.W., Demmig-Adams, B., Rosenstiel T.N., and Ebbert, V. (2001) Dependence of photosynthesis and energy dissioation activity upon growth form and light environment during the winter. Photosynthesis Research 67:51-62. Allen, J.D., Noguès, S. and Baker, N.R. (1998) Ozone depletion and increased UV-B radiation: is there a real threat to photosynthesis? Journal of Experimental Botany 49(328):1775-1788. Ambrosio, N.D, Arena, C. and Santo, A. (2003) Different relationship between electron transport and CO2 assimilation in two Zea mays cultivars as influenced by increasing irradiance. Photosynthetica 41(4):489-495. Bailey, S., Horton, P., and Walters, R. G. (2004) Acclimation of Arabidopsis thaliana to the light environment:the relationship between photosynthetic function and chloroplast composition. Planta 218:793-802. Ball, M.C., Butterworth, J.A., Roden, J.S., Christian, R., and Egerton, J.J.G. (1994) Application of chlorophyll fluorescence to forest ecology. Australian Journal of Plant Physiology 22:311-319. Berg, A.K., and Perkins, T.D. (2004) Evaluation of a portable chlorophyll meter to estimate chlorophyll and nitrogen contents in sugar maple (Acer saccharum Marsh.) leaves. Forest Ecology and Management 200:113-117. Bergmeyer H.U. (1983) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim Chen, L., and Cheng, L. (2003) Both xanthophylls cycle-dependent thermal dissipation and the antioxidant system are up- regulated in grape (Vitis labrusca L. cv. Concord) leaves in response to N limitation. Journal of Experimental Botany 54:2165-2175. Cheng, L. (2003) Xanthophyll cycle pool size and composition in relation to the nitrogen content of apple leaves. Journal of Experimental Botany 54:385-393. Cheng, L., Fuchigami, L.H., and Breen, P.J. (2000) Light absorption and partitioning in relation to nitrogen content in ‘Fuji’ apple leaves. Journal of American Society of Horticulture Science 125:581-587. Close, D.C., Beadle, C.L., and Hovenden, M.J. (2001) Cold-induced photoinhibition and foliar pigment dynamics of Eucalyptus nitens seedings during establishment. Australian Journal of Plant Physiology 28:113-1141. Dai, X.B., Xu, X.M., Lu, W., and Kuang, T.Y. (2003) Photoinhibition characteristics of a low chlorophyll b mutant of high yield rice. Photosynthetica 41(1):57-60. Demmig-Adams, B. (2003) Linking the xanthophylls cycle with thermal energy dissipation. Photosynthesis Research 76:73-80. Demmig-Adams, B. and Adams, III W.W. (1992) Photoprotection and other responses of plants to hight light stress. Annual Review of Plant Physiology 43:599-626. Demmig-Adams, B., and Adams, III W.W. (1996a) The role of xanthophylls cycle carotenoids in the protection of photosynthesis. Trends in Plant Science 1:21-26. Demmig-Adams, B., and Adams, III W.W. (1996b) Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among highter plant species. Planta 198:460-470. Demmig-Adams, B., Adams, III W.W., Barker, D.H., Logan, B.A., Bowlong, D.R., and Verhoeven, A.S. (1996) Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excitation. Physiolgia Plantarum 98:253-264. Demmig, B., and Winter, K. (1988) Characterisation of three components of non-photochemical fluorescence quenching and their response to photoinhibition. Australian Journal of Plant Physiology 15:163-177 Dodd, I.C., Critchley, C., Woodall, G.S., and Strewart, G.R. (1998) Photoinhibition in differently coloured juvenile leaves of Syzygium species. Journal of Expermental Botany 49(325):1437-1445. Evain, S., Flexas, J., and Moya, I. (2004) A new instrument for passive remote sensing : 2 Measurement of leaf and canopy reflectance changes at 531 nm and their relationship with photosynthesis and chlorophyll fluorescence. Remote Sensing of Environment 91:175-185. Feng, Y.L., Cao, K.F., and Feng, Z.L. (2002) Thermal dissipation, leaf rolling and inactivation of PSII reaction centres in Amomum villosum. Journal of Tropical Ecology 18:865–876. Gamon, J.A., Field, C.B., Bilger, W., Björkman, O., Fredeen, A.L. and Peñuelas, J. (1990) Remote sensing of the xanthophylls cycle and chlorophyll fluorescence in sunflower leaves and canopies. Oecologia 85:1-7. Gamon, J.A., Field, C.B., Fredeen, A.L., and Thayer, S. (2001) Assessing photosynthetic downregulation in sunflower stands with an optically-based model. Photosynthesis Research 67:113-125. Gamon, J.A., Peñuelas, J., and Field, C.B. (1992) A narrow-waveband spectral index that tracks diurnal changes in photosynthetic efficiency. Remote Sensing of Environment 41:35-44. Gamon, J.A., Serrano, L., and Surfus, J.S. (1997) The photochemical reflectance index: an optical indicator of photosymthetic radiation use efficiency across species, functional types, and nutrient levels. Oecologia 112:492-501. Gamon, J.A., and Surfus, J.S. (1999) Assessing leaf pigment content and activity eith a reflectometer. New Phytologist 143:105-117. García-Plazaola J.I., T. Faria, J. Abadía, A. Abadía, M.M. Chaves, and J.S. Pereira. 1997. Seasonal changes in xanthophylls composition and photosynthesis of cork oak (Quercus suber L.) leaves under Mediterranean climate. Journal of Expermental Botany 48(314):1667-1674. García-Plazaola, J.I., Olano, J.M., Hernández, A., and Becerril, J.M. (2003) Photoprotection in evergreen Mediterranean plants during udden periods of intense cold weather. Trees 17:285-291. Gitelson, A.A., Gritz, Y.and Merzlyak, M.N. (2003) Relationship between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. Journal of Plant Physiology 160: 271-282. Gitelson, A.A., and Merzlyak, M.N. (2004) Non-destructive assessment of chlorophyll, carotenoid and anthocyanin content in higher plant leaves: Principles and algorithms. Remote Sensing for Agriculture and the Environment, Greece, Ella, 2004:78-94. Gonzalez, R., Paul, N.D., Percy, K., Ambrose, M., McLaughlin, C.K., Barnes J.D., Aress, M. and Wellburn, A.R. (1996) Responses to ultraviolet-B radiation(280-315 nm) of pea(Pisu sativum) lines differing in leaf surface wax. Physiologia Plantarum 98:852-860. Grant, R.H., Heisler, G.M., Gao, W. and Jenks, M. (2003) Uitraviolet leaf reflectance of common urban trees and the prediction of reflectance from leaf surface characteristics. Agricultural and Forest Meteorology 120:127-139. Grasses, T., Pesaresi, P., Schiavon, F., Varotto, C., Salamini, F., Jahns, P., and Leister, D. (2002) The role of delta pH-dependent dissipation of excitation energy in protecting photosystem II against light-induced damage in Arabidopsis thaliana. Plant Physiology and Biochemistry 40:41–49. Guo, Y.H., and Cao, K.F. (2004) Effect of night chilling on photosynthesis of two coffee species grown under different irradiances. Journal of horticultural Science Biotechnology 79:713-716. Haimeirong and Kubota, F. (2003) The effects of drought stress and leaf ageing on leaf photosynthesis and electron transport in photosystem 2 in sweet potato (Ipomoea batatas Lam.) cultivars. Photosynthetica 41:253-258. Havaux, M., Bonfils, J. P., Lutz, C., and Niyogi, K.K. (2000) Photodamage of the photosynthetic apparatus and its dependence on the leaf developmental stage in the npq1 Arabidopsis mutant deficient in the xanthophylls cycle enzyme violaxanthin de-epoxidase. Plant Physiology 124:273-284. He, J., Chee, C.W., and Goh, C.J. (1996) ‘Photoinhibition’ of Heliconia under natural tropical conditions: the importance of leaf orientation for light interception and leaf temperature. Plant Cell and Environment 19:1238-1248. Holt, N.E., Zigmantas, D., Valkunas, L., Li, X.P., Niyogi, K.K., and Fleming, G.R. (2005) Carotenoid cation formation and the regulation of photosynthetic light harvesting. Science 307:433-436. Horton, P., and Ruban, A.V. (2005) Molecular design of the photosystem II light-harvesting antenna: photosynthesis and photoprotection. Journal of Experimental Botany 411:365–373. Hughes, N.M., Neufeld, H.S. and Burkey, K.O. (2005) Functional role of anthocyanins in high-light winter leaves of the evergreen herb Galax urceolata. New Phytologist 168:575-587 Kamfenkel, K., Montagu, M.V., and Inze, D. (1995) Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Analytical Biochemistry. 225:165-167. Kato, M.C., Hikosaka, K., and Hirose, T. (2002) Photoinactivation and recovery of photosystem II of Chenopodium album leaves grown under different irradiance and nitrogen availability. Functional Plant Biology 29:787-795. Kiato, M.C., Hikosaka, K., Hirotsu, N., Makino, A., and Hirose, T. (2003) The excess light energy that in neither utilized in photosynthesis nor dissipated by photoprotective mechanisms determines the rate of photoinactivation in photosystem II. Plant Cell Physiology 44:318-325. Kitao, M., Lei, T.T., Tobita, H., and Maruyama, Y. (2000a) Susceptibility to photoinhibition of three deciduous broadleaf tree species with different successional traits raised under various light regimes. Plant Cell and Environment 23:81-89. Kitao, M., Lei, T.T., Tobita, H., Maruyama, Y., and Ang, L.H. (2000b) Temperature response and photoinhibition investigated by chlorophyll fluorescence measurement for four distinct species of dipterocarp trees. Physiologia Plantarum. 109:284-290. Kurasová, I., Cajánek, M., Kalina, J., and Špunda (2000) Analysis of qualitative contribution of assimilatory and non-assimilatory de-excitation processes to adaptation of photosynthetic aapratus of barely plants to high irradiance. Photosynthetica 38 (4):513-519. Leverenz, J.W., Oquist, G.., and Wingsle, G. (1992) Photosynthesis and photoinhibition in leaves of chlorophyll b-less barley in relation to absorbed light. Physiologia Plantarum 85:495-502. Li, X.P., Björkman, O., Shih, C., Grossman, A.R., Rosenquist, M., Jansson, S., and Niyogi, K.K. (2000) A pigment-binding protein essential for regulation of photosynthetic light harvesting. Nature 403:391-395. Li, X.P., Müller-Moulé, P., Gilmore, A.M., and Niyogi, K.K. (2002) PsbS-dependent enhancement of feedback de-excitation protects photosystem II from photoinhibition. Pnas 99:15222–15227. Lichtenthaler, H. K. (1987) Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. Methods in Enzymology 148:350-382. Lichtenthaler, H.K., Buschmann, C. and Knapp, M. (2005) How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll fluorescence decrease ratio Rfd of leaves with the PAM fluorometer. Photosynthetica 43:379-393. Lin, Z.F., Peng, C.L., Lin, G.Z., Ou, Z.Y., Yang, C.W., Zhang, J.L. (2003) Photosynthetic characteristics of two new chlorophyll b-less rice mutants. Photosynthetica 41:61-67. Logan, B.A., Demmig-Adams, B., Adams, WW Ⅲ., and Grace, S.C. (1998) Antioxidant and Xanthophyll cycle-dependent energy dissipation in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field. Journal of Experimental Botany 49:1869-1879. Long, S.P., Humphries, S., and Falkowski, P.G. (1994) Photoinhibition of photosynthesis in nature. Annual Review of Plant Physiology 45:633-662. Lu, C., and Zhang, J. (2000) Photosynthetic CO2 assimilation, chlorophyll fluorescence and photoinhibition as affected by nitrogen deficiency in maize plants. Plant Science 151:135-143. Manetas, Y., Grammatikopoulos, G. and Kyparissis, A. (1998) The use of portable, non-destru- ctive, SPAD-502(Minolta) chlorophyll meter with leaves of varying trichome density and anthocyanin content. Journal of Plant Physiology 153: 513-516. Markham, K.R. (1982) Techniques of flavonoid identification. London: Academic Press. Maxwell, K., and Johoson, G.N. (2000) Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany 51:659-668. Merzlyak, M.N., Gitelson, A.A., Chivkunova, O.B., Solovchenko, A.E., and Pogosyan, S.I. (2003a) Application of reflectance spectroscopy for analysis of higher plant pigments. Russian Journal of Plant Physiology 50: 785-792. Merzlyak, M.N., Solovchenko, A.E. and Gitelson, A.A., (2003b) Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit. Postharvest Biology and Technology 27: 197-211. Méthy, M. (2000) Analysis of phytosynthetic activity at the leaf and canopy levels from reflectance measurements: a case study. Photosynthetica 38(4):505-512. Moran, J.A., Mitchell, A.K., Goodmnson, ., and Stockburger, K.A. (2000) Diferentiation among effecs of nitrogen fertilization treatments on coifer seedlings by foliar reflectance: a comparison of methods. Tre Physiology 20:1113-1120. Müller-Moulé, P., Golan, T., and Niyogi, K.K. (2004) Ascorbate-deficient mutants of Arabidopsis grow in high light despite chronic photooxidative stress. Plant physiol. 134:1163–1172. Müller, P., Li, X.P., and Niyogi, K.K. (2001) Non-photochemical quenching. Aresponse to excess light energy. Plant Physiology 125:1558-1566. Nakaji, T., Oguma, H., and Fjinuma, Y. (2006) Seasonal changes in the relationship between photochemical reflectance index and photosynthetic light use efficiency of Japanese larch needles. International Journal Remote Sensing 27:493-509. Nichol, C.J, rascher, u., Matsubara, S. and Osmond, B. (2006) Assessing photosynthetic efficiency in an experimental mangrove canopy using remote sensing and chlorophyll fluorescence. Tree 20:9-15. Niyogi, K.K. (1999) Photoprotection revisted: Genetic and molecular approaches. Annual Review of Plan Physiology 50:333-359. Niyogi, K.K., Grossman, A.R., and Björkman, O. (1998) Arabidopsis mutants define a central role for the xanthophyll cycle in the regulation of photosynthetic energy conversion. Plant Cell 10:1121–1134. Niyogi, K.K., Li, X.P., Rosenberg, V., and Jung, H.S. (2004) Is PsbS the site of non-photochemical quenching in photosynthesis? Journal of Experimental Botany 56:375-382. Osmond, C.B., and Grace, S.C. (1995) Perspectives on photoinhibition and photorespiration in the field: quintessential inefficiencies of the light and dark reactions of photosynthesis. Journal of Experimental Botany 46:1351-1362. Pandey, S., Kumry, S., and Nagar, P.K. (2003) Photosynthetic performance of Ginkgo bilioba L. grow under high and low irradiance. Photosynthetica 41 (4):505-511. Peng, C.L., Duan, J., Lin, G. and Glimore, A.M. (2002) Correlation between photoinhibition sensitivity and the rates and relative extents of xanthophylls cycle de-epoxidation in chlorine mutants of barley (H0rdeum bulgare L.) Photosynthetica 40(4):503-508. Peñuelas, J., Filella, I., and Gamon, J.A. (1995) Assessment of photosynthetic radiation use efficiency with spectral reflectance. New Phytology 131:291-296. Peñuelas, J., Filella, I., Llusià, J., Siscart, D., and Piñol, J. (1998) Comparative field study of spring and summer leaf gas exchang and photobiology of the Mediterranean trees Quercus ilex and Phillyrea latifolia. Journal of Experimental Botany 49(319):229-238. Peñuelas, J., and Inoue, Y. (1999) Reflectance indices indicative of changes in water and pigment contents of peanut and wheat leaves. Photosynthetica 36:355-360. Pfündel, E., and Bilger, W. (1994) Regulation and the possible function of the violaxanthin cycle. Photosynthesis Research 42:89-109. Pietrini, F., Iannelli, M.A., and Massacci, A., (2002) Anthocyanin accumulation in the illuminated surface of maize leaves enhances protection from photo-inhibitory risks at low temperature, without further limitation to photosynthesis. Plant, Cell and Environment 25(10):1251-1259. Robberecht, R., Caldwell, M.M., and Billings, W.D., (1980) Leaf ultraviolet optical properties along a latitudinal gradient in the arctic-alpine life zone. Ecology 61: 612-619. Sims, D.A., and Gamon, J. A. (2002) Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages. Remote Sensing Environment 81:337-354. Solhaug, K.A., and Haugen, J. (1998) Seasonal variation of photoinhibiton of photosynthesis in bark from Populus tremula L. Photosynthetica 35:411-417. Špundova, M., Strzalka, K., and Naus, J. (2005) Xanthophyll cycle activity in detached barley leaves senescing under dark and light. Photosynthetica 43 (1):117-124. Štroch, M., Cajanek, M., Kalina, J., and Spunda, V. (2004) Regulation of the excitation energy utilization in the photosynthetic apparatus of chlorina f2 barley mutant grown under different irradiances. Journal of Photochemistry and Photobiology 75:41–50. Stylinski, C.D., Gamon, J.A., and Oechel, W.C. (2002) Seasonal patterns of reflectance indices, carotenoid pigments and photosynthesis of evergreen chaparral species. Oecologia 131:366-374. Taiz, L., and Zeiger, E. (2002) plant physiology 3rd ed. pp 114, 137-162.Sinaure Associates Inc., Sunderland, Massaxhusetts. Takahashi, S., Tamashiro, A., Sakihama, Y., Yamamoto, Y., Kawamitsu Y. and Yamasaki, H. (2002) High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L. f. cv. Golden Leaves. BMC Plant Biology 2:1471-1478 Tardy, F., and Havaux, M. (1997) Thylakoid membrane fluidity and thermostability during the operation of the xanthophyll cycle in higher-plant chloroplasts. Biochimica et Biophysica Acta 1330:179-193. Tomek, P., Ilík, P., Lazár, D., Štroch, M., and Nauš, J. (2003) On thedetermintio of QB-non-reducing potosystem II centers from chlorophyll a fluorescence induction. Plant Science 164:665-670. Tsonev, T.D., and Hikosaka, K. (2003) contribution of photosynthetic electron transport, heat dissipation, and recovery of photoinactivated photosystem II to photoprotection at different temperatures in Chenopodium album leaves. Plant Cell Physiology 44:828-835. Verhoeven, A.S., Adams, III W.W., and Demmig-Adams, B. (1998) Two forms of sustained xanthopylls cycle-dependent energy dissipation in overwintering Euonymus kiautschovicus. Plant Cell Environment 21:893-903. Verhoeven, A.S., Demmig-Adams, B., and Adams, WW Ⅲ. (1997) Enhanced employment of the xanthophyll cycle and thermal energy dissipation in spinach exposed to high light and N stress. Plant physiology 113:817-824. Weng, J.H., Chen, Y.N., and Liao, T.S. (2006a) Relationaships between chlorophyll fluorescence parameters and photochemical reflectance index of tree species adapted to different temperature regimes. Functional Plant Biology 33:241-246. Weng, J.H., Liao, T.-S., Hwang, M.Y., Chung, C.C., Lin, C.P., and Chu, C.H. (2006b) Seaasonal variation in photosystem II efficiency and photochemical reflectance index of evergreen trees and perennial grasses growing at low and high elevations in subtropical Taiwan. Tree Physiology 26:1097-1104. Weng, J.H., Liao, T.S., Sun, K.H., Chung, J.C., Lin, C.P., and Chu, C.H. (2005) Seasonal variations in photosynthesis of Picea morrisonicola growing in the subalpine region of subtropical Taiwan. Tree Physiology 25:973-979. Weng, J.H., and Hsu, F.H. (2001) Gas exchange and epidermal characteristics of Miscanthus populations in Taiwan varying with habitats and nitrogen application. Photosynthetica 39:35-41. Winkel, T., Méthy, M., and Thénot, F. (2002) Radiation use efficiency, chlorophyll fluorescence, and reflectance indices associated with ontogenic changes in water-limited Chenopodium quinoa leaves. Photosynthetica 40(2):227-232. Xu, D.Q., Chen, X.M., Zhang, L.X., Wang, R.F., and Hesketh, J.D. (1993) Leaf photosynthesis and chlorophyll fluorescence in a chlorophyll-deficient soybean mutant. Photosynthetica 29:103-112. Yoder, B.J. and Waring, R.H. (1994) The normalized difference vegetation index of small Douglas-fir canopies with varying chlorophyll concentration. Remote Sensing Environment 49: 81-91.|
|摘要:||為了知悉不同葉色甘藷之葉綠素螢光及反射光譜特性，選用黃葉、綠葉及紫葉等三種不同葉色之甘藷為對象加以探討。結果顯示黃葉甘藷的葉綠素（Chla+b）、類胡蘿蔔素（Caro）、抗壞血酸（AsA）含量與其在2000μmolm-2s-1PPFD下的光合速率均較綠葉甘藷低，且天線較小，而葉綠素a/b、Caro/Chl值與不活化的PSII比率均較高。三種不同葉色甘藷葉片，其在400-700 nm波段的反射與穿透率均隨著葉綠素含量的增加而降低，其中以黃葉最高，綠葉次之，紫葉最低。而反射光譜指數 [（R750-800 / R695-740）-1] 及 [（R750–R705）/（R750+R705）]，均可準確的推估三種不同葉色甘藷葉片之Chla+b含量。在人工環境下的測定結果顯示，在固定溫度與光度條件下，多肥處理的黃葉與綠葉甘藷，其光化學消散比率（P）較缺肥者高，而非光化學消散比率（D）則較低。此外，中肥處理者，其P會隨著溫度的上升而增加，而D則反之，此乃缺肥或低溫處理使其光化學消散能力降低，而提高非光化學消散，以防止過剩光能對葉片的傷害。在低溫（10℃）高光（2000μmolm-2s-1）處理30 min後，黃葉甘藷的非光化學消散（NPQ）與高能狀態消散（qE）均較較綠葉甘藷為低，而光抑制消散（qI）則較高。因此黃葉甘藷在10℃下以2000μmolm-2s-1照射30 min，關燈後其光系統二最大潛能（Fv/Fm）的回升速率較低。此外，在自然條件下的測定結果顯示，三種葉色甘藷的黎明Fv/Fm及光化學反射指數 [PRI =（R531–R570）/（R531+R570）] 值會隨測定當日最低溫之下降而減少，其中以黃葉甘藷尤劇。中午之Fv/Fm及∆F/Fm''均會隨測定當日PPFD增加而降低，三種葉色甘藷∆F/Fm''之降幅均大致相近，而Fv/Fm之降幅則是黃葉甘藷較大，尤以高PPFD下為甚。此外在相同的NPQ下，黃葉甘藷之快速非光化學消散（NPQf）較綠葉與紫葉甘藷為小，且在相同的P下，黃葉甘藷之D較小，而過剩光能比率（E）較大，導致黃葉甘藷光抑制較嚴重，即在低溫高光下Fv/Fm降幅較大。其因可能為，黃葉甘藷由於Caro及AsA含量均較低，影響其非光化學消散與抗氧化能力所致。比較黃葉與綠葉甘藷中午之∆F/Fm''及NPQ與標準化後的∆PRI [（黎明PRI－中午PRI）/ 黎明PRI or中午PRI ] 間之相關係數均較PRI為高。因此，標準化後的∆PRI適用於黃葉與綠葉甘藷的光合成效能及非光化學消散之推估。|
In order to understand the chlorophyll fluorescence and reflectance spectral characteristics of leaves with different leaf color, 3 sweet potato (Ipomoea batatas) cultivars (yellow-green, green and purple) were used to study. The results indicated that the contents of chlorophyll (Chl a+b), carotenoid (Caro), ascorbate (AsA) and the photosynthetic capacity (measured at 2000 μmolm-2s-1 PPFD) as well as antenna size of yellow-green cultivar were lower, but Chl a/b, Caro/Chl and inactive PSII was higher than those of green cultivar. The reflection and transmission rates of leaves were higher in yellow-green, followed green and lower in purple cultivar, and thease two rates increasing with the increase of chlorophyll content. And the Chl a+b content of three cultivars could be estimated from the leaf reflectance spectra index [(R750-800/R695-740)-1] and [(R750-R705)/(R750+R705)]. Under controlled temperature and irradiance conditions, the fraction of light absorbed in PSII antennae that is utilized in photosynthetic electron transport (P) of yellow-green and green cultivar under high-level fertilizer was higher than no fertilizer, while the fraction of light absorbed in PSII antennae that is dissipated via thermal energy dissipation in the antennae (D) was just the opposite. Under middle-level fertilizer, P was increased but D was decreased with the increase of temperature. That was low nitrogen and low temperature could reduce P, sweet potato leaf might up-regulate its D to avoid the damage due to excess light energy. Under low temperature (10℃) and high irradiance (2000 μmolm-2s-1) for 30 minutes, the non-photochemical quenching (NPQ) and the energy-dependent quenching (qE) of yellow-green was lower, but the photoinhibition quenching (qI) was higher than those of green cultivar. There fore, under low temperature and high light, the maximum photochemical efficiency of photosystem II (Fv/Fm) was lower in yellow-green cultivar. Under natural conditions, it also showed that, at predawn, Fv/Fm value and photochemical reflectance index [PRI=(R531-R570)/(R531+R570)] were reduced with the decrease of minimum temperature of measured day, and the decrease of Fv/Fm and PRI were most drastic in yellow-green cultivar. At midday Fv/Fm and ΔF/Fm' were reduced with the increase of PPFD, and yellow-green cultivar showed a drastic decline in Fv/Fm especially under high PPFD. Compared at a same level of NPQ, the fast non-photochemical quenching (NPQf) of yellow-green cultivar was lower than that of green and purple cultivars. In addition, yellow-green cultivar also showed lower D and the fraction of excess absorbed in PS II antennae (E) when compared at a same level of P, and leaded to the higher degree of photoinhibition under lower temperature and higher light conditions. It might due to the lower contents of Caro and AsA in yellow-green cultivar leading lower NPQf and antioxidation. Compared to the relationships between two fluorescence parameters (ΔF/Fm' and NPQ) and PRI, higher regression coefficient could be found between two fluorescence parameters and normalized ΔPRI [(PRI morning – PRI noon) / PRI morning or PRI noon]. Thus the normalized ΔPRI was more fitted for estimate the efficiency of photosynthesis and non-photochemical quenching in yellow-green and green cultivars.
在 DSpace 系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。