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標題: 阿拉伯芥RTNLB2與RTNLB4蛋白質於土壤農桿菌感染植物過程之功能分析
Functional study of the AtRTNLB2 and AtRTNLB4 proteins in Agrobacterium-mediated plant transformation process
作者: 張耀仁
Chang, Yao-Ren
關鍵字: arabidopsis
出版社: 生命科學系所
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摘要: 土壤農桿菌(Agrobacterium tumefaciens)為一植物病原菌。於感染時會將菌體內Ti質體(tumor-inducing plasmid)上的一段特定DNA片段(T-DNA,transferred-DNA)及Vir蛋白質(virulence protein)轉移進入植物細胞內,T-DNA則會嵌入植物的染色體中,造成植物產生腫瘤(crown gall),進而影響植物的生長。農桿菌利用由一穿膜的蛋白質複合體及T線毛所組成的第四型分泌系統(T4SS,type IV secretion system),將單股的T-DNA及Vir蛋白質運送至植物細胞內。T線毛是由VirB2、VirB5及VirB7蛋白質所組成。為了進一步瞭解T線毛是如何參與在農桿菌的感染過程中,本研究針對可與VirB2結合的二個植物蛋白質BTI2(RTNLB2)及BTI3(RTNLB4)進行更深入的研究。實驗結果顯示rtnlb2-2及rtnlb4-3阿拉伯芥突變株,較不易被農桿菌感染。當RTNLB4大量表現於轉殖植物時,則可增加其被農桿菌轉殖的效率。由此得知RTNLB2及RTNLB4,皆有參與在農桿菌轉殖植物的過程中。於阿拉伯芥中共有15個RTNLB基因,但對於其功能還未有深入地瞭解。使用生物晶片實驗結果所累積的資料庫得知,多數的RTNLB基因可普遍表現於植物的各個組織器官中。另外,當植物細胞受到環境逆境及賀爾蒙處理時,多數的RTNLB基因可受其誘導或抑制基因的表現量。由此推測RTNLB蛋白質對於植物生長及發育相當重要。但RTNLB蛋白質於植物中所扮演的角色為何,仍須進一步的分析和研究。
The Agrobacterium tumefaciens is a plant pathogen. It causes crown gall disease on plants by transferring its T-DNA (transferred DNA) from the Ti (tumor-inducing) plasmid into plant cells and integrating into the plant chromosome. The A. tumefaciens utilizes the type IV secretion system (T4SS) that includes a transmembrane protein complex and a filament structure, T-pilus, to transfer virulence proteins and the single strand T-DNA into plant cells. The T-pilus is composed of VirB2, VirB5, and VirB7 proteins. In order to understand how the T-pilus involved in the A. tumefaciens infection process, two of the VirB2-interacting proteins, BTI2 (RTNLB2) and BTI3 (RTNLB4), in plants were further characterized in this study. Two Arabidopsis T-DNA insertion mutants, rtnlb2-2 and rtnlb4-3, are resistant to Agrobacterium-mediated transformation process. RTNLB4 over-expression transgenic plants also show higher transformation efficiencies. These data further support that both RTNLB2 and RTNLB4 are involved in A. tumefaciens infection process. There are 15 RTNLB genes in Arabidopsis, and their functions in plant growth and development remain unknown so far. Results collected from various microarray analyses show that most of the RTNLB genes ubiquitously expressed in various tissues, including roots, leaves, flowers and so on. Additionally, several of the RTNLB genes are down- or up-regulated by environmental stresses and hormone treatments based on the microarray analysis results, suggesting RTNLBs may play important roles in plants. The possible functions of RTNLBs in plants await further investigations.
其他識別: U0005-1912200819381700
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