Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22767
標題: 反玉米素合成蛋白質對土壤農桿菌致病力及生長影響之功能分析
Functional studies of the Tzs protein in Agrobacterium tumefaciens virulence and growth
作者: 李宜霖
Lee, Ying-Ling
關鍵字: Agrobacterium tumefaciens
土壤農桿菌
virulence gene
trans-zeatin synthesis protein
trans-zeatin
tzs gene
vir基因
反玉米素合成蛋白質
反玉米素
tzs基因
出版社: 生命科學系所
引用: Agrios G N 2004 Plant pathology. 5th ed. Elsevier Academic Press, Burlington, MA, USA. Akiyoshi D E, Klee H, Amasino R M, Nester E W and Gordon M P 1984 T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc Natl Acad Sci USA 81, 5994-5998. Akiyoshi D E, Morris R O, Hinz R, Mischke B S, Kosuge T, Garfinkel D J, Gordon M P and Nester E W 1983 Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci USA 80, 407-411. Akiyoshi D E, Regier D A and Gordon M P 1987 Cytokinin production by Agrobacterium and Pseudomonas spp. J Bacteriol 169, 4242-4248. Akiyoshi D E, Regier D A, Jen G and Gordon M P 1985 Cloning and nucleotide sequence of tzs gene from Agrobacterium tumefaciens strain T37. Nucleic Acids Res 13, 2773-2788. Aly K A, Krall L, Lottspeich F and Baron C 2008 The type IV secretion system component VirB5 binds to the trans-zeatin biosynthetic enzyme Tzs and enables its translocation to the cell surface of Agrobacterium tumefaciens. J Bacteriol 190, 1595-1604. Anand A, Uppalapati S R, Ryu C M, Allen S N, Kang L, Tang Y and Mysore K S 2008 Salicylic acid and systemic acquired resistance play a role in attenuating crown gall disease caused by Agrobacterium tumefaciens. Plant Physiol 146, 703-715. Angra-Sharma R and Sharma D K 1999 Cytokinins in pathogenesis and disease resistance of Pyrenophora teres-barley and Dreschslera maydis-maize interactions during early stages of infection. Mycopathologia 148, 87-95. Ashby A M, Watson M D, Loake G J and Shaw C H 1988 Ti plasmid-specified chemotaxis of Agrobacterium tumefaciens C58C1 toward vir-inducing phenolic compounds and soluble factors from monocotyledonous and dicotyledonous plants. J Bacteriol 170, 4181-4187. Ashby A M, Watson M D and Shaw C H 1987 A Ti plasmid determined function is responsible for chemotaxis of Agrobacterium tumefaciens towards the plant wound product acetosyringone. FEBS Microbiol Lett 41, 189-195. Astot C, Dolezal K, Nordstrom A, Wang Q, Kunkel T, Moritz T, Chua N-H and Sandberg G 2000 An alternative cytokinin biosynthesis pathway. Proc Natl Acad Sci USA 97, 14778-14783. Atmakuri K, Ding Z and Christie P J 2003 VirE2, a Type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens. Mol Microbiol 49, 1699-1713. Backert S and Meyer T F 2006 Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol 9, 207-217. Bako L, Umeda M, Tiburcio A F, Schell J and Koncz C 2003 The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants. Proc Natl Acad Sci USA 100, 10108-10113. Ballas N and Citovsky V 1997 Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein. Proc Natl Acad Sci USA 94, 10723-10728. Baron C 2005 From bioremediation to biowarfare: on the impact and mechanism of type IV secretion systems. FEMS Microbiol Lett 253, 163-170. Barry G F, Rogers S G, Fraley R T and Brand L 1984 Identification of a cloned cytokinin biosynthetic gene. Proc Natl Acad Sci USA 81, 4776-4780. Beaty J S, Powell G K, Lica L, Regier D A, MacDonald E M S, Hommes N G and Morris R O 1986 Tzs, a nopaline Ti plasmid gene from Agrobacterium tumefaciens associated with trans-zeatin biosynthesis. Mol Gen Genet 203, 274-280. Brencic A and Winans S C 2005 Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol Mol Biol Rev 69, 155-194. Buchanan B B, Gruission W and Jones R L 2000 Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, MD, USA. Bulgakov V P, Kiselev K V, Yakovlev K V, Zhuravlev Y N, Gontcharov A A and Odintsova N A 2006 Agrobacterium-mediated transformation of sea urchin embryos. Biotechnol J 1, 454-461. Bundock P, den Dulk-Ras A, Beijersbergen A and Hooykaas P J 1995 Trans- kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14, 3206-3214. Bundock P, Mroczek K, Winkler A A, Steensma H Y and Hooykass P J 1999 T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis. Mol Gen Genet 261, 115-121. Cangelosi G A, Martinetti G, Leigh J A, Lee C C, Theines C and Nester E W 1989 Role for Agrobacterium tumefaciens ChvA protein in export of beta-1,2-glucan. J Bacterial 171, 1609-1615. Carlier A, Chevrot R, Dessaux Y and Faure D 2004 The assimilation of gamma-butyrolactone in Agrobacterium tumefaciens C58 interferes with the accumulation of the N-acyl-homoserine lactone signal. Mol Plant Microbe Interact 17, 951-957. Cascales E and Christie P J 2004 Definition of a bacterial type IV secretion pathway for a DNA substrate. Science 304, 1170-1173. Chai Y, Tsai C S, Cho H and Winans S C 2007 Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens. J Bacteriol 189, 3674-3679. Chevrot R, Rosen R, Haudecoeur E, Cirou A, Shelp B J, Ron E and Faure D 2006 GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A 103, 7460-7464. Christie P J, Atmakuri K, Krishnamoorthy V, Jakubowski S and Cascales E 2005 Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu Rev Microbiol 59, 451-485. Christie P J and Cascales E 2005 Structural and dynamic properties of bacterial type IV secretion systems. Mol Membr Biol 22, 51-61. Christie P J, Ward J E, Gordon M P and Nester E W 1989 A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proc Natl Acad Sci USA 86, 9677-9681. Christie P J, Ward J E, Winans S C and Nester E W 1988 The Agrobacterium tumefaciens virE2 gene product is a single-stranded-DNA-binding protein that associates with T-DNA. J Bacteriol 170, 2659-2667. Cho H and Winans S C 2005 VirA and VirG activate the Ti plasmid repABC operon, elevating plasmid copy number in response to wound-released chemical signals. Proc Natl Acad Sci USA 102, 14843-14848. Citovsky V, De Vos G, Zambryski P 1988 Single-stranded DNA binding protein encoded by virE locus of Agrobacterium tumefaciens. Science 240, 501-504. Citovsky V, Guralnick B, Simon M N and Wall J S 1997 The molecular structure of Agrobacterium VirE2-single stranded DNA complexes involved in nuclear import. J Mol Biol 271, 718-727. Citovsky V, Kozlovsky S V, Lacroix B, Zaltsman A, Dafny-Yelin M, Vyas S, Tovkach A and Tzfira T 2007 Biological systems of the host cell involved in Agrobacterium infection. Cell Microbiol 9, 9-20. Citovsky V, Wong M L, and Zambryski P 1989 Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process. Proc Natl Acad Sci USA 86, 1193-1197. Citovsky V, Zupan J, Warnick D, Zambryski P 1992 Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science 256, 1802-1805. Dang T A, Zhou X R, Graf B and Christie P J 1999 Dimerization of the Agrobacterium tumefaciens VirB4 ATPase and the effect of ATP-binding cassette mutations on the assembly and function of the T-DNA transporter. Mol Microbiol 32, 1239-1253. Das A 1988 Agrobacterium tumefaciens virE operon encodes a single-stranded DNA-binding protein. Proc Natl Acad Sci USA 85, 2909-2913. De Groot M J, Bundock P, Hooykaas P J and Beijersbergen A G 1998 Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16, 839-842. DeCleene M and DeLey J 1976 The host range of crown gall. Bot Rev 42, 389-466. Deng W, Chen L, Peng W T, Liang X, Sekiguchi S, Gordon M P, Comai L, Nester E W 1999 VirE1 is a specific molecular chaperone for the exported single-stranded-DNA-binding protein VirE2 in Agrobacterium. Mol Microbiol 31, 1795-1807. Deng W, Chen L, Wood D W, Metcalfe T, Liang X, Gordon M P, Comai L and Nester E W 1998 Agrobacterium VirD2 protein interacts with plant host cyclophilins. Proc Natl Acad Sci USA 95, 7040-7045. Dombek P and Ream W 1997 Functional domains of Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2. J Bacteriol 179, 1165-1173. Durrenberger F, Crameri A, Hohn B and Koukolikova-Nicola Z 1989 Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation. Proc Natl Acad Sci USA 86, 9154-9158. Eberl L, Winson M K, Sternberg C, Stewart G S, Christiansen G, Chhabra S R, Bycroft B, Williams P, Molin S and Givskov M 1996 Involvement of N-acyl-L-hormoserine lactone autoinducers in controlling the multicellular behaviour of Serratia liquefaciens. Mol Microbiol 20, 127-136. Eisenbrandt R, Kalkum M, Lai E M, Lurz R, Kado C I and Lanka E 1999 Conjugative pili of IncP plasmids, and the Ti plasmid T pilus are composed of cyclic subunits. J Biol Chem 274, 22548-22555. Eisenbrandt R, Kalkum M, Lurz R and Lanka E 2000 Maturation of IncP pilin precursors resembles the catalytic Dyad-like mechanism of leader peptidases. J Bacteriol 182, 6751-6761. Filichkin S A and Gelvin S B 1993 Formation of a putative relaxation intermediate during T-DNA processing directed by the Agrobacterium tumefaciens VirD1, D2 endonuclease. Mol Microbiol 8, 915-926. Friesner J and Britt A B 2003 Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and defective in T-DNA integration. Plant J 34, 427-440. Gallego M E, Bleuyard J Y, Daoudal-Cotterell S, Jallut N and White C I 2003 Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis. Plant J 35, 557-565. Gao R and Lynn D G 2005 Environmental pH sensing: resolving the VirA/VirG twocomponent system inputs for Agrobacterium pathogenesis. J Bacteriol 187, 2182-2189. Gao R, Mukhopadhyay A, Fang F and Lynn D G 2006 Constitutive activation of two-component response regulators: characterization of VirG activation in Agrobacterium tumefaciens. J Bacteriol 188, 5204-5211. Gelvin S B 2000 Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Physiol Plant Mol Biol 51, 223-56. Gelvin S B 2003 Agrobacterium mediated plant transformation: the biology behind the 〝gene -jockeying〞tool. Microbiol Mol Biol Rev 67, 16-37. Gelvin S B 2006 Agrobacterium transformation of Arabidopsis thaliana roots: a quantitative assay. Methods Mol Biol 343, 105-113. Gouka R J, Gerk C, Hooykaas P J, Bundock P, Muster W, Verips C T and de Groot M J 1999 Transformation of Aspergillus awamori by Agrobacterium-mediated homologus recombination. Nat Biotechnol 17, 598-601. Hajdukiewicz P, Svab Z and Maliga P 1994 The small versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25, 989-994. Herrera-Estrella A, Chen Z, Van Montagu M and Wang K 1988 VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA protein complex at the 5’ terminus of T-strand molecules. EMBO J 7, 4055-4062. Hiei Y, Ohta S, Komari T and Kumashiro T 1994 Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6, 271-282. Hooykaas P J and Schilperoort R A 1992 Agrobacterium and plant genetic engineering. Plant Mol Biol 19, 15-38. Howard E and Citovsky V 1990 The emerging structure of the Agrobacterium T-DNA transfer complex. BioEssays 12, 103-108. Hwang H H and Gelvin S B 2004 Plant proteins that interact with VirB2, the Agrobacterium tumefaciens pilin protein, mediate plant transformation. Plant Cell 16, 3148-3167. Howard E A, Winsor B A, De Vos G and Zambryski P C 1989 Activation of the T-DNA transfer process in Agrobacterium results in the generation of a T-strand protein complex: tight association of VirD2 with the 5’ ends of T-strands.Proc Natl Acad Sci USA 86, 4017-4021. Howard E A, Zupan J R, Citovsky V and Zambryski P C 1992 The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell 68, 109-118. Jarchow E, Grimsley N H and Hohn B 1991 virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays. Proc Natl Acad Sci USA 88, 10426-10430. Jayaswal R K, Veluthambi K, Gelvin S B and Slightom J L 1987 Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by virD-encoded border-specific endonuclease from Agrobacterium tumefaciens. J Bacteriol 169, 5035-5045. Jin S, Roitsch T, Ankenbauer R G, Gordon M P and Nester E W 1990 The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation. J Bacteriol 172, 525-530. John M C and Amasino R M 1988 Expression of an Agrobacterium Ti plasmid gene involved in cytokinin biosynthesis is regulated by virulence loci and induced by plant phenolic compounds. J Bacteriol 170, 790-795. Jones A L, Lai E M, Shirasu K and Kado C I 1996 VirB2 is a processed pilin-like protein encoded by the Agrobacterium tumefaciens Ti plasmid. J Bacteriol 178, 5706-5711. Jones S, Yu B, Bainton N J, Birdsall M, Bycroft B W, Chhabra S R, Cox A J, Golby P, Reeves P J, Stephens S, Winson, M K, Salmond, G P C, Stewart, G S A B and Williams, P 1993 The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J 12, 2477-2482. Kado, C I and Heskett M G 1970 Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopathology 60, 969-976. Kemp J D 1978 In vivo synthesis of crown gall-specific Agrobacterium tumefaciens-directed derivatives of basic amino acids. Plant Physiol 62, 26-30. Krall L, Raschke M, Zenk M H and Baron C 2002 The Tzs protein from Agrobacterium tumefaciens C58 produces zeatin ribose 5’-phosphate from 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate and AMP. FEBS (Fed Eur Biochem Soc) Lett 527, 315-318. Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C and Citovsky V 2001 Genetic transformation of Hela cells by Agrobacterium. Proc Natl Acad Sci USA 98, 1871-1876. Lacroix B, Li J, Tzfira T and Citovsky V 2006 Will you let me use your nucleus? How Agrobacterium gets its T-DNA expressed in the host plant cell. Can J Physiol Pharmacol 84, 333-345. Lai E M and Kado C I 1998 Processed VirB2 is the major subunit of the promiscuous pilus of Agrobacterium tumefaciens. J Bacteriol 180, 2711-2717. Lai E M, Shih H W, Wen S R, Cheng M W, Hwang H H and Chiu S H 2006 Proteomic analysis of Agrobacterium tumefaciens response to the Vir gene inducer acetosyringone. Proteomics 6, 4130-4136. Letham D S 1973 Cytokinin from Zea mays. Phytochemistry 12, 2445-2455. Li J, Krichevsky A, Vaidya M, Tzfira T and Citovsky V 2005 Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium. Proc Natl Acad Sci USA 102, 5733-5738. Lichter A, Barash I, Valinsky L and Manulis S 1995a The genes involved in cytokinin biosynthesis in Erwinia herbicola pv. gypsophilae: characterization and role in gall formation. J Bacteriol 177, 4457-4465. Lichter A, Manulis S, Sagee O, Gafni Y, Gray J, Meilan R, Morris R O and Barash I 1995b Production of cytokinin by Erwinia herbicola pv. gypsophilae and isolation of a locus conferring cytokinin biosynthesis. Mol Plant Microbe Interact 8, 114-121. Liu P and Nester E W 2006 Indoleacetic acid, a product of transferred DNA, inhibits vir gene expression and growth of Agrobacterium tumefaciens C58. Proc Natl Acad Sci U S A 103, 4658-4662. McCullen C A and Binns A N 2006 Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer. Annu Rev Cell Dev Biol 22, 101-127. Miller C O, Skoog F, Von Saltza M H and Strong F 1955 Kinetin, a cell division factor from deoxyribonucleic acid. J AM Chem Soc 77, 1392-1393. Montoya A L, Chilton M D, Gordon M P, Sciaky D and Nester E W 1977 Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. J Bacteriol 129, 101-107. Montoya A L, Moore L W, Gordon M P and Nester E W 1978 Multiple genes coding for octopine-degrading enzymes in Agrobacterium. J Bacteriol 136, 909-915. Mysore K S Nam J and Gelvin S B 2000 An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration. Proc Natl Acad Sci USA 97, 948-953. O′Connell K P and Handelsman J 1989 chvA locus may be involved inexport of neutral cyclic beta-1,2-linked D-glucan from Agrobacterium tumefaciens. Mol Plant Microbe Interact 2, 11-16. Parsek M R, Val D L, Hanzelka B L, Cronan J E, Jr. and Greenberg E P 1999 Acyl homoserine-lactone quorum-sensing signal generation. Proc Natl Acad Sci U S A 96, 4360-4365. Piper K R, Beck von Bodman S and Farrand S K 1993 Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362, 448-450. Piper K R, Beck Von Bodman S, Hwang I and Farrand S K 1999 Hierarchical gene regulatory systems arising from fortuitous gene associations: controlling quorum sensing by the opine regulon in Agrobacterium. Mol Microbiol 32, 1077-1089. Piper K R and Farrand S K 2000 Quorum sensing but not autoinduction of Ti plasmid conjugal transfer requires control by the opine regulon and the antiactivator TraM. J Bacteriol 182, 1080-1088. Powell G K, Hommes N G, Kuo J, Castle L A and Morris R O 1988 inducible expression of cytokinin biosynthesis in Agrobacterium tumefaciens by plant phenolics. Mol Plant Microbe Interact 1, 235-242. Powell G K and Morris R O 1986 Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin biosynthetic gene: homology with Agrobacterium tumefaciens tmr and tzs loci. Nucl Acids Res 14, 2555-2565. Rashkova S, Spudich G M and Christie P J 1997 Characterization of membrane and protein interaction determinants of the Agrobacterium tumefaciens VirB11 ATPase. J Bacteriol 179, 583-591. Rashkova S, Zhou X R, Chen J and Christie P J 2000 Self-assembly of the Agrobacterium tumefaciens VirB11 traffic ATPase. J Bacteriol 182, 4137-4145. Rossi L, Hohn B and Tinland B 1996 Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens. Proc Natl Acad Sci USA 93, 126-130. Sakakibara H 2005 Cytokinin biosynthesis and regulation. Vitam Horm 72, 271-287. Sakakibara H 2006 Cytokinin activity, biosynthesis, and translocation. Annu Rev Biol 57, 431-449. Schagger H and von Jagow G 1987 Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1-100 kDalton. Ana Biochem 166, 368-379. Schmidt-Eisenlohr H, Domke N, Angerer C, Wanner G, Zambryski P C and Baron C 1999 Vir proteins stabilize VirB5 and mediate its association with the T-pilus of Agrobacterium tumefaciens. J Bacteriol 181, 7485-7492. Schroder G, Waffenschmidt S, Weiler E W and Schroder J 1984 The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem 138, 387-391. Segulenko V, Saqulenko E, Jakubowski S, Spidich E and Christie P J 2001 VirB7 lipoprotein is exocellular and associates with the Agrobacterium tumefaciens T pilus. J Bacteriol 183, 3642-3651. Sen P, Pazour G J, Anderson D, Das A 1989 Cooperative binding of Agrobacterium tumefaciens VirE2 protein to single-stranded DNA. J Bacteriol 171, 2573-2580. Shaw C H, Ashby A M, Brown A, Royal C and Loake G J 1988 virA and virG are the Ti-plasmid functions required for chemotaxis of Agrobacterium tumefaciens towards acetosyringone. Mol Microbiol 2, 413-417. Stachel S E and Zambryski P C 1986 virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens. Cell 46, 325-333. Sunderg C D and Ream W 1999 The Agrobacterium tumefaciens chaperone-like protein, VirE1, interacts with VirE2 at domains required for single-stranded DNA binding and cooperative interaction. J Bacteriol 181, 6850-6855. Surico G, Iacobellis N S and Sisto A 1985 Studies on the role of indole-3-acetic acid and cytokinins in the formation of knots on olive and oleander plants by Pseudomonas syringae pv. savastanoi. Physiol Plant Pathol 26, 309-320. Sykorova B, Kuresova G, Daskalova S, Trckova M, Hoyerova K, Raimanova I, Motyka V, Travnickova A, Elliott M C and Kaminek M 2008 Senescence-induced ectopic expression of the A. tumefaciens ipt gene in wheat delays leaf senescence, increases cytokinin content, nitrate influx, and nitrate reductase activity, but does not affect grain yield. J Exp Bot 59, 377-387. Taiz L and Zeiger E 2002 Plant physiology. 4th ed. Sinauer Associates, Inc., Sunderland, MA, USA. Tao Y, Rao P K, Bhattacharjee S and Gelvin S B 2004 Expression of plant protein phosphatase 2C interferes with nuclear import of the Agrobacterium Tcomplex protein VirD2. Proc Natl Acad Sci USA 101, 5164-5169. Tinland B, Koukolikova-Nicola Z, Hall M N and Hohn B 1992 The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signal. Proc Natl Acad Sci USA 89, 7442-7446. Tinland B, Schoumacher F, Gloeckler V, Bravo-Angel, A M and Hohn B 1995 The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome. EMBO J 14, 3585-3595. Thomashow L S, Reeves S and Thomashow M F 1984 Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. Proc Natl Acad Sci USA 81, 5071-5075. Thomashow M F, Hugly S, Buchholz W G and Thomashow L S 1986 Molecular basis for the auxin-indepdent phenotype of crown gall tumor tissues. Science 231, 616-618. Thomashow M F, Karlinsey J E, Marks J R and Hurlbert R E 1987 Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment. J Bacteriol 169, 3209-3216. Toothman P 1982 Octopine accumulation early in crown gall development is progressive. Plant Physiol 69, 214-219. Toro N, Datta A, Carmi O A, Young C, Prusti R K and Nester E W 1989 The Agrobacterium tumefaciens virC1 gene product binds to Overdrive, a T-DNA transfer enhancer. J Bacteriol 171, 6845-6849. Tzfira T and Citovsky V 2008 Agrobacterium: from biology to biotechnology. Springer Science+Business Media, LLC., New York, NY, USA. Tzfira T, Rhee Y, Chen M H, Kunik T and Citovsky V 2000 Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls. Annu Rev Microbiol 54, 187-219. Tzfira T, Vaidya M and Citovsky V 2001 VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity. EMBO J 20, 3596-3607. Tzfira T, Vaidya M and Citovsky V 2002 Increasing plant susceptibility to Agrobacterium infection by overexpression of the Arabidopsis nuclear protein VIP1. Proc Natl Acad Sci USA 99, 10435-10440. Tzfira T, Vaidya M and Citovsky V 2004 Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431, 87-92. Van Attikum H, Bundock P and Hooykaas P J J 2001 Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration. EMBO J 20, 6550-6558. Van Attikum H, Bundock P, Overmeer R M, Lee LY, Gelvin S B and Hooykaas P J J 2003 The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA. Nucleic Acids Res 31, 4247-4255. Van Onckelen H, Prinsen E, Inze D, Rudeisheim P, Van Lijsebettens M, Follin A, Schell J, Van Montagu M and De Greef J 1986 Agrobacterium T-DNA gene 1 codes for tryptophan 2-monooxygenase activity in tobacco crown gall cells. FEBS (Fed Eur Biochem Soc) Lett 198, 357-360. Vergunst A C, van Lier M C, den Dulk-Ras A, Stuve T A, Ouwehand A and Hooykaas P J 2005 Positive charge is an important feature of the C-terminal transport signal of the VirB/D4-translocated proteins of Agrobacterium. Proc Natl Acad Sci USA 102, 832-837. Wang K 2006 Agrobacterium protocols. 2nd ed. Humana Press, Totowa, NJ, USA. Wang K, Herrera-Estrella A and Van Montagu M 1990 Overexpression of virD1 and virD2 genes in Agrobacterium tumefaciens enhances T-complex formation and plant transformation. J Bacteriol 172, 4432-4440. Wang K, Stachel S E, Timmerman B, Van Montagu M and Zambryski P C 1987 Site specific nick in the T-DNA border sequence as a result of Agrobacterium vir gene expression. Science 235, 587-591. Ward E R and Barnes W M 1988 VirD2 protein of Agrobacterium tumefaciens very tightly linked to the 5’ end of T-strand DNA. Science 242, 927-930. Werner T, Motyka V, Strand M and Schmulling T 2001 Regulation of plant growth by cytokinin. Proc Natl Acad Sci USA 98, 10487-10492. Yanofsky M F, Porter S G, Young C, Albright L M, Gordon M P and Nester E W 1986 The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease. Cell 47, 471-477. Yi H, Mysore K S and Gelvin SB 2002 Expression of the Arabidopsis histone H2A-1 gene correlates with susceptibility to Agrobacterium transformation. Plant J 32, 285-298. Young C and Nester E W 1988 Association of the VirD2 protein with the 5’ end of T-strands in Agrobacterium tumefaciens. J Bacteriol 170, 3367-3374. Yuan Z C, Edlind M P, Liu P, Saenkham P, Banta L M, Wise A A, Ronzone E, Binns A N, Kerr K and Nester E W 2007 The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium. Proc Natl Acad Sci USA 104, 11790-11795. Yusibov V M, Steck T R, Gupta V, Gelvin S B 1994 Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. Proc Natl Acad Sci USA 91, 2994-2998. Zhan X C, Jones D A and Kerr A 1990 The pTiC58 tzs gene promotes high-efficiency root induction by agropine strain 1855 of Agrobacterium rhizogenes. Plant Mol. Biol. 14, 785-792. Zhang H B, Wang L H and Zhang L H 2002 Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A 99, 4638-4643. Zhang L, Murphy P J, Kerr A and Tate M E 1993 Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature 362, 446-448. Zhu Y, Nam J, Humara J M, Mysore K S, Lee L Y, Cao H, Valentine L, Li J, Kaiser A D, Kopecky A L, Hwang H H, Bhattacharjee S, Rao P K, Tzfira T, Rajagopal J, Yi H, Veena, Yadav B S, Crane Y M, Lin K, Larcher Y, Gelvin M J, Knue M, Ramos C, Zhao X, Davis S J, Kim S I, Ranjith-Kumar C T, Choi Y J, Hallan V K, Chattopadhyay S, Sui X, Ziemienowicz A, Matthysse A G, Citovsky V, Hohn B and Gelvin S B 2003 Identification of Arabidopsis rat mutants. Plant Physiol 132, 494-505. Ziemienowicz A, Tinland B, Bryant J, Gloeckler V and Hohn B 2000 Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro. Mol Cell Biol 20, 6317-6322. Zupan J R, Ward D and Zambryski P 1998 Assembly of the VirB transport complex for DNA transfer from Agrobacterium tumefaciens to plant cells. Curr Opin Microbiol 1, 649-655.
摘要: 土壤農桿菌(Agrobacterium tumefaciens)為植物病原菌,其感染過程是利用VirA/VirG兩蛋白質所構成的two-component系統所啟動及調節。此系統可辨認自植物根部傷口所分泌出的酚類化合物(例如:乙醯丁香酮, AS),並誘導表現位在Ti 質體上的vir基因(virulence genes),進而使得T-DNA傳遞進入植物細胞,最後嵌入植物基因體中表現。野生種農桿菌的T-DNA上含有iaaH和iaaM及ipt基因,iaaH和iaaM基因會使受感染的植物細胞,產生過多的吲哚乙酸(Indoleacetic acid﹐IAA),而ipt基因則促使寄主細胞,產生過多的細胞分裂素(cytokinin),以致植物產生冠癭狀腫瘤。在nopaline系的農桿菌Ti質體上,含有一與ipt基因同源的tzs基因,其表現亦受乙醯丁香酮和VirA/VirG two-component系統所調節。tzs基因轉譯合成反玉米素合成蛋白質(Tzs: trans-zeatin synthesis protein),可使農桿菌在乙醯丁香酮誘導下產生並分泌反玉米素(trans-zeatin)。目前研究已知Tzs蛋白質在AS誘導下,會被運送到土壤農桿菌的細胞膜上。故本研究針對Tzs蛋白質是否參與土壤農桿菌與植物交互作用,及其於感染過程中,所扮演的角色做進一步分析。本研究結果顯示當農桿菌的tzs基因突變後,會造成其在與阿拉伯芥切根共培養時生長遲緩,同時造成其阿拉伯芥根部細胞後產生腫瘤和短暫表現T-DNA效率亦下降。且經由基因及化學互補實驗得知,推測tzs突變株因缺乏細胞分裂素而導致其感染力下降。並發現當使用低濃度的野生種農桿菌感染阿拉伯芥時,外加細胞分裂素可增加其短暫表現T-DNA的效率與細菌的生長。另外,當土壤農桿菌的tzs基因突變後,會造成其於19℃或25℃酸性的AB-MES培養基中,並加入乙醯丁香酮誘導培養下生長遲緩,並使得其VirB2、VirD2和VirE2蛋白質的表現量比野生株高。由本研究結果可知農桿菌的tzs基因或反玉米素的分泌,於農桿菌感染植過過程中,可能扮演著重要的角色。
Agrobacterium tumefaciens is a pathogen and is capable of infecting many kinds of plants and few other eukaryotic species. When plant wounded sites secrete phenolic compounds, such as acetosyringone (AS), and under acidic conditions, the VirA/VirG two-component system induces virulence gene expressions from the Ti plasmid (tumor-inducing plasmid), and transfers the transferred DNA (T-DNA) into plant cells, and finally integrated into plant genomes. In the wild type Agrobacterium, its T-DNA region contains iaaH, iaaM, and ipt genes. The iaaH and iaaM genes lead to the productions of indoleacetic acid (IAA) and the ipt gene leads to the productions of cytokinin in the infected plant cells, which causes crown gall diseases in plants. In the nopaline type Ti plasmid, there is a tzs gene which shares the sequence similarity with the ipt gene. The tzs gene expression is induced by acetosyringone and regulated by the VirA/VirG two-component system. The tzs gene encoded the trans-zeatin synthesizing protein (Tzs) can promote trans-zeatin productions and secretions in Agrobacterium. In order to explore further the importance and the roles of the tzs gene, we utilized the tzs deletion and frame-shift mutants to determine if the tzs gene is involved in the Agrobacterium virulence. Our data demonstrated that tzs deletion and frame-shift mutants showed growth defects and reduced the stable and transient transformation efficiencies when infected Arabidopsis roots. Data from genetic and chemical complementation tests showed that reduced infection efficiencies can be complemented by exogenous cytokinins. Additionally, supplements of cytokinin during infections slightly enhanced the growth and infection ability of lower concentrations of the wild-type Agrobacterium. Our data also revealed that tzs mutants showed growth defects when grown in acidic AB-MES medium with AS at either 19℃ or 25℃, and caused higher VirB2, VirD2, and VirE2 protein expressions compared to the wild type. Taken together, our data suggest that the tzs gene and/or trans-zeatin secretions may play an important role during infections.
URI: http://hdl.handle.net/11455/22767
其他識別: U0005-2508200815540200
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2508200815540200
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