Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23121
標題: 公雞睪丸再生之分析
Analysis of chicken testis regeneration
作者: 鄭伯亮
Cheng, Po-Liang
關鍵字: Chicken
公雞
testis
regeneration
睪丸
再生
出版社: 生命科學系所
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摘要: 摘要 對公雞進行去勢手術在華人養雞產業中是一項歷史悠久的傳統。然而在切除不完全的狀況下,遺留在體內的小部分殘留組織,會持續發育並保有產生精子的能力。許多研究顯示生殖幹細胞可以重建Spermatogenesis,而另外有研究發現將公雞去勢後,移植小塊睪丸至皮下可以附著、發育並且可以產生有功能的精子[2]。一般認為再生應為在器官或組織受到傷害之後,可以經歷再一次的發育過程,復原為原本大小或類似原大小且具有正常功能的器官或組織,然而不論是殘留在公雞體內或移植到皮下的睪丸是屬於發育或是再生並未界定,而且睪丸在移植後發育成熟的過程是否和一般體內的發育相同並不清楚。我們的實驗將切除下的睪丸移植至皮下,用來觀察公雞性腺的再生過程,並研究其生長調控的機制。 觀察自體移植睪丸生長的情形,結果顯示自體移植睪丸的重量增加倍率明顯比正常發育的睪丸多。在經過12週的生長之後,可以從自體移植睪丸中得到具有活動力的精子。由此可以認為自體移植的睪丸有進行再生,並且回復原本的產精功能。進一步比較正常發育睪丸與自體移植睪丸的各細胞型態生長情形,我們利用Sox9標記發育中Sertoli cell 和Leydig cell;以及WT1 和Aromatase分別標定成熟的Sertoli cell 和Leydig cell 來探討各細胞型態在睪丸再生過程中增生與分化的狀態。在5,6週時可以開始發現有生殖細胞在增生,第7-9週體細胞(Leydig 和Sertoli cell)進行大量增生,進入第十週後體細胞的增生開始減緩,而生殖細胞則開始進行減數分裂,到了12週開始有精細胞的出現。在成功再生的自體移植睪丸內,也可以觀察到有與正常發育睪丸類似的細胞型態分佈。
Abstract Regeneration is a process that reactivates developmental programs in post-embryonic life to restore missing tissues. Chicken testis are known to regenerate in a small population of castrated males, however no detailed documentation of this unique phenomenon has being reported. We developed an auto-transplantation system that by inserting a small piece of testicular tissue under the skin can allow regeneration to occur. By continuous monitoring the development of transplanted testicular, we found the transplanted testicular tissue grow much faster than the endogenous un-castrated testes and the growth rate continues till after week 30. The histological analysis revealed slightly distorted seminiferous tubules, but the major cell types: Sertoli cells, different stages of developing sperm and Spermatogonia, and the interstitial Leydig cells are all present; furthermore we are able to collect motile sperm from this auto-grafted testicular tissue, indicating the functionality of seminiferous tubules are not damaged. According these results, we conclude that the transplanted chicken testicular tissues have regenerative ability.
URI: http://hdl.handle.net/11455/23121
其他識別: U0005-2308201016393900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2308201016393900
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