Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23156
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dc.contributor羅婉瑜zh_TW
dc.contributorWan-Yu Loen_US
dc.contributor蔡淑娟zh_TW
dc.contributor曾登裕zh_TW
dc.contributor吳淑美zh_TW
dc.contributorShu-ChuanTsaien_US
dc.contributorDeng-Yu Tsengen_US
dc.contributorShu- Mei Wuen_US
dc.contributor.advisor李宗翰zh_TW
dc.contributor.advisorTsung-Han Leeen_US
dc.contributor.author賴東洋zh_TW
dc.contributor.authorLai, Dung-Yangen_US
dc.contributor.other中興大學zh_TW
dc.date2011zh_TW
dc.date.accessioned2014-06-06T07:19:41Z-
dc.date.available2014-06-06T07:19:41Z-
dc.identifierU0005-2807201002310600zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/23156-
dc.description.abstract摘要 墨綠凹鼻魨 (Tetraodon nigroviridis)為棲息於河口的廣鹽性魚類,可適應於海水及淡水,先前的研究已從墨綠凹鼻魨中發現FXYD家族中的一員,命名為pFXYD9,並發現在長期馴化至淡、海水環境時,鰓上pFXYD9在mRNA及蛋白表現量為淡水高於海水,和鰓上Na+, K+-ATPase(NKA)活性及蛋白質含量趨勢相反,顯示pFXYD9 以抑制的方式調控NKA活性。本研究將長期馴養於海水(高張)環境中的魚直接轉移至淡水(低張),透過偵測鰓上pFXYD9基因及蛋白量的變化,搭配NKA蛋白量及活性的趨勢,以及血液滲透壓、氯離子濃度和肌肉含水量等滲透壓調節相關的生理反應,探討滲透壓調節過程中pFXYD9蛋白質所扮演的角色,來了解墨綠凹鼻魨面臨低張環境時的生理機制。 利用共同免疫沉澱法實驗,發現免疫反應在轉移後的7個時間點皆測出NKA的表現,判斷在適應低張環境期間pFXYD9和NKA兩蛋白質在鰓上持續彼此之間的交互作用。由NKA活性及pFXYD9蛋白質表現量的結果發現,兩者在各時間點的表現並沒有相對應,NKA蛋白質含量在轉到淡水24小時後便持平表現,pFXYD9蛋白質含量顯著變化的下一段時間,NKA活性也會隨之產生抑制的變動,推測鰓上pFXYD9蛋白質以帶動性的模式影響NKA活性。 生理方面,在轉移12小時後血液滲透壓和血液中氯離子濃度出現顯著性低點,鈉離子濃度也在24小時明顯下降,隨著體內離子濃度及滲透壓降低,鰓上pFXYD9蛋白質含量和NKA活性開始顯著性變化,NKA活性48小時明顯下降、在96小時顯著升高,血液滲透壓及離子濃度在96小時回升, NKA活性也藉由pFXYD9蛋白質的影響在轉移後168小時顯著下降,由此顯示墨綠凹鼻魨在適應低張環境時,藉由鰓上pFXYD9蛋白質以帶動性影響NKA活性的模式,協助墨綠凹鼻魨調控滲透壓來達成體內恆定。zh_TW
dc.description.tableofcontents目錄 中文摘要 ................................................. i 英文摘要 ................................................ ii 目錄 .................................................... iv 圖表目錄 ................................................ vi 前言 ..................................................... 1 材料與方法 ............................................... 8 結果 .................................................... 20 討論 .....................................................24 參考文獻 ................................................ 30 圖表目錄 Fig. 1. Changes in plasma osmolality of pufferfish directly transferred from SW to FW..............42 Fig. 2. Changes in of plasma Na+ concentrations pufferfish directly transferred from SW to FW................43 Fig. 3. Changes in plasma Cl- concentrations of pufferfish directly transferred from SW to FW................44 Fig. 4. The muscle water contents of pufferfish transfer directly from SW to FW............................45 Fig. 5.Branchial NKA activity of pufferfish transferred directly from SW to FW.............................46 Fig. 6. The representative immunoblot of NKA α-subunit and relative protein abundance in gills...............47 Fig. 7. Expression of relative pFXYD9 mRNA abundance in gill of pufferfish................................48 Fig. 8. The representative immunoblot of pFXYD9 and protein abundance in gill of pufferfish...................49 Fig. 9. Co-immunoprecipitation of pFXYD9 with Na+/K+-ATPase protein...........................................50zh_TW
dc.language.isoen_USzh_TW
dc.publisher生命科學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2807201002310600en_US
dc.subjectTetraodon nigroviridisen_US
dc.subject墨綠凹鼻魨zh_TW
dc.subjectNKAen_US
dc.subjectpFXYD9en_US
dc.subject滲透壓調節zh_TW
dc.title廣鹽性墨綠凹鼻魨(T. n.)適應低張環境時的生理反應zh_TW
dc.titleThe physiological responses of hyposmotic shock on the euryhaline pufferfish, Tetraodon nigroviridisen_US
dc.typeThesis and Dissertationzh_TW
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