Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23194
標題: 鋅化合物對聖約翰草生長及有效成分影響之 研究
Study on effects of Zinc-compounds on growth and effective ingredients in St. John's Wort (Hypericum perforatum)
作者: 張家綸
Jhang, Jia-Luen
關鍵字: H. perforatum
聖約翰草
nano-scale ZnO
microwave equipment
Hyp1
PAL
奈米氧化鋅
微波裝置
出版社: 生命科學院碩士在職專班
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摘要: 中文摘要 本研究以聖約翰草為材料,施加奈米氧化鋅和不同鋅化合物來探討對聖約翰草生長發育及有效成份的影響。結果發現,在試驗處理條件下,三星期後皆可達到近60%以上的發芽率。當光強度在350 (µmole m-2s-1)以上或光週期超過6小時的處理,可增加hypericin的產生,且光強度與植物鮮重的大小有著明顯的關係存在。以最適生長條件施加各種鋅化合物,濃度在5mM以上皆會造成減產甚至植物死亡。Hyp1活性方面,濃度以2.5mM、5mM處理者皆可誘導酵素活性的表現;而PAL活性方面,發現水份逆境或不同鋅化合物處理皆明顯誘導PAL的活性表現,結果顯示利用Hyp1能夠調控hypericin成份的產生,而PAL酵素因為有許多機制相互影響,其與成份的生合成關係不易觀察。微波萃取方面:使用甲醇溶劑在750W的功率下,50分鐘內能快速且有效的萃取各種指標成份,萃取溶劑選擇方面,hypericin以甲醇萃取約10分鐘就達到不錯的萃取效果,而hyperforin來說,以ethyl acetate萃取約5分鐘就可達到不錯的效果,最後使用一種HPLC分析條件可在60分鐘內成功地分析並定量6種指標成分。綜合以上結果,使用5mM濃度的鋅化合物能有效來調控植物的生長。但除了hypericin外,對其他有效成分之產量尚未找出其關連性。因此思考未來如何更有效利用鋅化合物來提高聖約翰草活性成分的產率就有其重要性,或許也可以評估作為土壤復育植物的潛力,藉此開發更多聖約翰草的經濟應用價值。 關鍵字:聖約翰草、奈米氧化鋅、微波裝置、Hyp1、PAL、Hypericin、Hyperforin、Pseudohypericin、Quercetin、Quercitrin、Rutin
Abstract In this study, the effects of nano-scale Zinc Oxide and different Zinc-compounds on effective ingredients, growth and development of St. John’s Wort have been investigated.The results show that the germination rate of Hypericum perforatum seeds can reach over 65% after 3 weeks under experimental conditions. The content of hypericin in H. perforatum increases under the treatment of light intensity at 350(µmole m-2s-1) and the photoperiod over 6h. In addition, the relationship between light intensity and plant fresh weight correlates obviously. The experiment reveals that considered by appropriate conditions for H. perforatum, all of Zinc-compounds (>5mM) will reduce yield and even lead to death of H. perforatum. For the activity of Hyp1, treatment with Zinc-compounds(both at 2.5mM and 5mM) can induce Hyp1 activity. The enzyme activity of PAL can be obviously induced under water stress or with different Zinc-compounds. The effect of study shows that Hyp1 was used to control the production of ingredients of hypericin. However, the relationship between PAL and the ingredient flavonols are not easily observed because they interact each other by many mechanisms. It has been shown that the rapid and effective microwave extraction of indicator ingredients of H. perforatum can be conducted at 750W in 50 minates through the use of methanol. For hypericin, methanol shows the satisfied extraction efficacy can be completed about 10 minates. Regarding hyperforin, satisfied extraction efficacy can be completed about 5 minates through the use of ethyl acetate. For HPLC analysis, by this analytic method, 6 indicator ingredients of H. perforatum can be successfully analysed and quantified respectively. Taken together,zinc-compounds at 5mM can be regulate the plant growth effectively. But it seems to have no correlation with the productivity of effective ingredients inbetween except hypericin.Therefore,it’s important to consider to use zinc-compounds more effectively to elevate production of active ingredients of H. perforatum in the future. It will be also able to evaluate St. John’s Wort as a phytoremedy of soil for its more diverse economic applications. Key words:H. perforatum、nano-scale ZnO、microwave equipment、Hyp1、PAL、 Hypericin、Hyperforin、Pseudohypericin、Quercetin、Quercitrin、Rutin.
URI: http://hdl.handle.net/11455/23194
其他識別: U0005-0502200722142700
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