Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23208
標題: 分析孕婦尿液,羊水及胎兒細胞中重金屬之濃度
The determination of heavy metal in concentrations of maternal urine, amniotic fluid and fetal cell
作者: 蔡麗華
Tasi, Li-Hua
關鍵字: 重金屬
Heavy metal
懷孕婦女
尿液
羊水
胎兒細胞
二手菸
染色體
母血唐氏症篩檢
超音波
pregnant women
urine
amniotic fluid
fetal cell
chromosome
Environmental tobacco smoke ( ETS )
Maternal serum screning for Down Syndrome ( MSS - DS )
ultrasound
出版社: 生命科學院碩士在職專班
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摘要: 環境因素是造成胎兒畸形的重要原因之一,其中以重金屬的暴露最為嚴重。重金屬以多種途徑進入人體,並可通過孕婦胎盤與臍帶進入胎兒體內,或長期蓄積在人體的器官中,影響核酸代謝及各種酶活性降低,導致氧化損傷危害人體健康及胎兒畸形、流產或死亡。環保署曾指出彰化縣境內農田遭重金屬暴露污染較嚴重,主要是受到銅、鉻、鎘、鉛、汞、砷、鎳、鋅等八種重金屬污染,然而此地區對懷孕婦女之重金屬暴露對健康影響的研究較少。故本研究之目的是測量懷孕婦女尿液、羊水、胎兒細胞中重金屬的含量,並探討各檢體重金屬之相關性及與胎兒染色體結果之相關性,總共收集中部某教學醫院婦產科門診,做羊膜穿刺術的孕婦,共33位,期間收集問卷並留其尿液、羊水及胎兒細胞。三種檢體均以感應耦合電漿質譜儀( ICP – MS )分析砷、鎘、鉻、銅、鎳、汞、鉛、鋅、硒等九種元素,所有檢體的測量均在嚴格的品質控制下執行。 結果顯示砷在各檢體中有統計上的相關性( r = 0.430 , r = 0.524 ),鉻、銅、汞在尿液與羊水間也具有統計相關性( r = 0.705 , r = 0.502 , r = 0.550 ),鎳則在尿液、胎兒細胞間具有統計相關性( r = 0.517 ),顯示母親體內的重金屬可能會透過胎盤和臍帶傳輸到胎兒體內。且孕婦在二手菸暴露組中發現尿液檢體中重金屬砷、鎘、汞、鉛、硒、Cu/Zn、Cu/Se,羊水檢體中重金屬濃度鎘、鋅、Cu/Zn、Se/Cd,胎兒細胞檢體中重金屬濃度鎘、鉻、鎳、硒皆具有顯著統計上的差異,尤其是胎兒細胞中重金屬濃度皆較高,顯示出二手菸暴露與孕婦之重金屬濃度有重要的相關性。染色體異常組中,年齡、母血唐氏症篩檢、超音波異常是其主要高危險群,但各檢體重金屬濃度差異不大,並無具有統計上顯著差異,此可能與研究樣本數不足或檢體中重金屬濃度較低有關。本研究已初步調查國內孕婦尿液、羊水、胎兒細胞之重金屬濃度,亦可有助益瞭解彰化縣地區暴露重金屬之污染,以降低對懷孕婦女及胎兒的危害,做為衛生及環保機關推展重金屬污染防治之參考。
The health effects of environmental pollutant are varied, heavy metal is one of pollutants through various exposure routes and cause embryonic developmental abnormalities. Body of pregnant woman''s in the toxins may be transported through placenta and the umbilical cord into uterus, and perhaps cause a miscarriage or embryonic effects. The Environmental Protection Administration (EPA) investigated the farmland pollution of heavy metal across the island in 2001. According to the report, heavy metal pollution was most serious in ChangHua county. Levels of copper, chromium, cadmium, lead, mercury, arsenic, nickel and zinc were commonly measured. Limited literatures were explored and assessed the health effects of heavy metal upon pregnant women in this area. The objective of this research is to measure the levels of heavy metal in urine and amniotic fluid as well as the fetal cells of the embryo among the pregnant women. 33 pregnant women were interviewed by a questionnaire and collected urine sample. Amniotic fluid and fetal cell samples were collected through amniocentesis in a hospital. Levels of arsenic, cadmium, chromium, copper, nickel, mercury, lead, zinc, and selenium in all specimens were analyzed using an inductively coupled plasma mass spectrometry ( ICP - MS ). Results showed urinary arsenic levels correlated with both in the amniotic fluid and the fetal cell sample, respectively ( r = 0.430, r = 0.524 ). The levels of chromium, copper, and mercury were found significant correlations between in the urine and the amniotic fluid ( r = 0.705, r = 0.502, r = 0.550 ), but not found in the fetal cell sample. Level of nickel in the fetal cell was significantly correlated with urinary nickel levels( r = 0.517 ). However, other heavy metals did not show any correlation in each specimen. Pregnant women exposed to environmental tobacco smoke (ETS) in their home or/and workplace were found the higher level of arsenic, chromium, mercury, lead, zinc, and selenium in specimens. Maternal serum screening for Down syndrome ( MSS - DS ) and reading results of ultrasound were not significantly different levels of metals in all specimens. The chromosomal abnormalities were significant correlated with age of pregnant women and abnormal finding by ultrasound. In conclusion, it is a significant issue to measure the levels of heavy metals in urine, amniotic fluid and fetal cell for the pregnant women from pollution area. Our finding can establish the background information of heavy metal in Taiwanese in order to understand the serious problems in heavy metals pollution of ChangHua county. Furthermore, this research will provide a reference to Taiwan EPA which can take action to reduce the pollution of heavy metal in environmental resources to alleviate the health effects in both the pregnant woman and embryo fetal.
URI: http://hdl.handle.net/11455/23208
其他識別: U0005-1801200717150500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1801200717150500
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