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標題: 台灣五種藥用植物增殖系統之建立
Establishment of multiplication protocol on five medicinal plants in Taiwan
作者: 陳信任
Chen, Shinn-Ren
關鍵字: medicinal plant
tissue culture
出版社: 生命科學院碩士在職專班
引用: 何陽修。1993。火鶴花、彩葉芋及非洲菊微體繁殖苗之馴化。國立中興大學園藝研究所碩士論文。台中。pp 74。 何曉明、王鳴、王喆之。1996。辣椒葉片離體培養與植株再生。西北農業大學學報24(1):93-96。中國。 林怡如、葉德銘。2004。出瓶馴化光度與培植期對‘綠巨人’白鶴芋組培苗出瓶生長之影響。中國園藝。50:21-30。 林宜信。2003。台灣藥用植物源名錄。行政院衛生署中醫藥委員會。pp188、278、418。 邱德文、吳家榮、夏同珩。2001。本草綱目彩色藥圖(上卷、中卷)。台北:薪傳出版社。pp233、319、664。 高木村。1988。台灣藥用植物手冊。台北:南天書局。pp150、244、251。 高景輝。1998。植物荷爾蒙生理。台北:華香園出版社。pp25-43。 張淑華、何政坤、蔡錦瑩。2004。台灣紅豆杉之細胞培養與紫杉烷類生產。台灣林業科學19: 43-52。 許鴻源。1972。台灣地區出產中藥藥材圖鑑。行政院衛生署中醫藥委員會。pp 53。 葉豐次、陳忠川、黃雯雯、那琦、蔡新聲。1994。恒春薯蕷之組織培養(二)細胞懸浮培養之建立及Diosgenin含量之測定。中華農藝4:257-268。 劉新裕、林俊義、張成國。2002。2002年藥用植物專輯。行政院農業委員會農業試所。pp51。 鄭武燦。2000。台灣植物圖鑑。台北。茂昌。pp1735、2205、2685。 Ahroni A., Zuker A., Rozen Y., Shejtman H. and Vainstein A. 1997. An efficient method for advertitious shoot regeneration from stem-segment explants of Gypsophila. Plant Cell Tiss. Org. Cult. 49: 101-106. Al-Bahrany A.M. 2002. Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing. Sci.Hort. 95:285-295. Alfermann A.W. and Petersen M. 1995. Natural products formation by plant cell biotechnology. Plant Cell Tissue Org. Cult. 43:199–205. Alvarez A., Pomar F., Sevilla M.A. and Montero M.I. 1999. Gastric antisecretory and antiulcer activities of an ethanolic extract of Bidens pilosa L. var. radiata Schult. Bip.J. Ethnopharmacol. 322: 333-340. Arnold S. V. and Eriksson T. 1984. Effects of agar concentration on growth and anatomy of adventitious of Picea abies L. Plant Cell Tiss. Org. Cult. 3:257-264. Arora R. and Bhojwani S.S.1989. In vitro propagation and low temperature storage of Saussurea lappa C.B. Clarke: an endangered medicinal plant. Plant Cell Rep. 8:44–7. Arumugam N. and Bhojwani S.S. 1990. Somatic embryogenesis in tissue cultures of Podophyllum hexandrum. Can. J. Bot. 68:487–91. Bajaj Y.P.S.1988. Regeneration of plants from frozen protoplasts of Atropa belladonna, Datura innoxia and Nicotiana tabacum. Ind. J. Exp. Biol. 26:289–92. Barna K.S. and Wakhlu A.K. 1988. Axillary shoot induction and plant regeneration in Plantago ovata Forssk. Plant Cell Tiss. Org. Cult. 15:169–73. Basu P. and Chand S. 1998. Regeneration of plantlets from root derived callus of egg plant henbane. Cell and Chromosome Res. 19(1):31–4. Beck M. J. and Camper N. D. 1991. Shoot regeneration from petunia leaf discs as a function of explant size, configuration and benzyladenine exposure. Plant Cell Tiss. Org. Cult. 26:101-106. Bellini C., Chupeau M-C, Gervais M., Vastra G. and Chupeau Y. 1990. Importance of myo-inositol, calcium, and ammonium for the viability and division of tomato (Lycopersicon esculentum) protoplast. Plant Cell Tiss. Org. Cult. 23: 27–37. Benjamin B.D., Roja P.C., Heble M.R. and Chadha M.S. 1987. Multiple shoot cultures of Atropa belladona: effect of physicochemical factors on growth and alkaloid formation. J. Plant Nutr. 129:129–35. Bhagyalakshmi N. and Singh N.S. 1988. Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of Oleoresin. J. Hort. Sci. 63(2):321–7. Bhagyalakshmi N. and Singh N.S. 1995. Role of liquid versus agar-gelled media in mass propagation and ex vitro survival in bananas. Plant Cell Tiss. Org. Cult. 41:71-73. Bhakuni D.S., Dhar M.L., Dhar M.M., Dhawan B.N. and Mehrotra B.N. 1969. Screening of Indian plants for biological activity: Part II. Indian Journal of Experimental Biology. 7:250–262. Brevoort P. 1998. The blooming U.S. botanical market: a new overview. Herbalgram. 44:33–46. Briskin D. and Hanson J.B. 1992. How does the plant plasma membrane HC-ATPase pump protons? J. Exp. Bot. 43: 269–289. Brown S., Wetherell D.F. and Dougall D.K. 1976. The potassium requirement for growth and embryogenesis in wild carrot suspension cultures. Physiol. Plant. 37: 73–79. Bush D.S. 1995. Calcium regulation in plant cells and its role in signalling. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46: 95–122. Cacho M., Moran M., Herrera M.T., Fernandez-Tarrago J. and Corchete M.P. 1991. Morphogenesis in leaf, hypocotyls and explants of Digitalis thapsi L. cultured in vitro. Plant Cell Tiss. Org. Cult. 25:117-123. Calamar A. and de Klerk G.J. 2002. Effect of sucrose on advertitious rot regeneration in apple. Plant Cell Tiss. Org. Cult. 70:207-212. Chen L.J., Hu T.W. and Huang L.C.1995. A protocol toward multiplication of the medicinal tree, Eucommia ulmoides Oliver. In vitro Cell Dev. Biol. Plant. 31: 193–8. Cox P.A. and Balick M.J. 1994. The ethanobotanical approach to drug discovery. Sci. Am. 82–87. Dornenburg H. and Knorr D. 1996. Production of the phenolic flavour compounds with cultured cells and tissues of Vanilla planifolia species. Food Biotechnol. 10:75–92. Dornenburg H. and Knorr D. 1997. Challenges and opportunities for metabolite production from plant cell and tissue cultures. Food Technol. 51:47- 54. Dunstan D., Turner K.E. and Lazaroff W.R. 1985. Propagation in vitro of the apple rootstock M4: Effect of phytohormones on shoot quality. Plant Cell Tiss. Org. Cult. 4:55-60. Economou A.S. 1987. Light treatment to improve efficiency of in vitro propagation systems. J. Japan. Soc. Hort. Sci. 22:751-754. Etienne H., Lartaud M.P., Carron M.P. and Michaux-Ferrière N. 1997. Use of calcium to optimize long-term proliferation of friable embryogenic calluses and plant regeneration in Hevea brasiliensis (Mull. Arg.). J. Exp. Bot. 48: 129–137. Faria R.T. and Illg R.D. 1995. Micropropagation of Zingiber spectabile Griff. Scientia Horticulturae. 62:135–7. Feron G., Bonnarme P., Durand A. 1996. Prospects for the microbial production of food flavours. Trends Food Sci. Technol. 7:285–293. Fowler M.W. and Scragg A.H. 1988. Natural products from higher plants and plant cell culture. In: Plant cell biotechnology. NATO ASI Series, vol. 18., eds. Pais M.S.S, Mavituna F. and Novais J.M., pp.165–177. Berlin: Springer-Verlag. Fukui H., Tani M. and Tabata M. 1990. Induction of shikonin biosynthesis by endogenous polysaccharides in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 9:73-76. Gamborg O.L., Miller R.A. and Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cell. Exp. Cell. Rep. 50:151-158. George E.F. and Sherrington P.D. 1984. Plant Propagation by Tissue Culture. In: Handbook and Directiory of Commercial Laboratories., pp.125-300. England: Eastern Press. Ghosh B. and Sen S. 1996. Suspension culture, somatic embryogenesis and stable regeneration in Asparagus cooperi Baker. Cytobios. 87:189–200. Glaser V. 1999. Billion-dollar market blossoms as botanicals take root. Nat. Biotechnol. 17:17–18. Grout B.W.W. and Donkin M.E. 1987. Photosynthetic activity of cauliflower meristem culture in vitro and at transplanting into soil. Acta. Hort. 212:323-327. Gupta D.R., Dhiman R.P., Naithani S. and Ahmed B. 1988. Chemical investigation of Bischofia javanica Blume. Pharmazie. 43:222. Harborne J.B.1999. Classes and functions of secondary products. In: Chemicals from Plants, Perspectives on Secondary Plant Products., eds. Walton N.J. and Brown D.E., pp. 1–25. Imperial College Press. Hartmann H.T. and Kester D.E. 1983. Plant propagation: principles and practices 4th, ed. Prentice H. Englewood Cliffs, NJ. Hasegawa A., Ohashi H. and Goi M. 1985. Effects of BA, rhizome length, mechanical treatment and liquid shaking culture on the shoot formation from rhizome in Cymbidium faberi Rolfe. Acta. Hortic. 166:25-40. Hasegawa K., Mizutani J., Kosemura S. and Yamamura S. 1992. Isolation and identification of lepidimoide, a new allelopathic substance from mucilage of germinated cress seeds. Plant Physiol. 100:1059-1061. Hayashi M., Kozai T. and Watanabe K. 1990. Effedts of CO2 enrichment and high solar radiation on the growth of potato plantlets in direct ex-vitro rooting method. Environ. Control Biol. 28(4):147-154. Heberle-Bors E. 1985. In vitro haploid formation from pollen: a critical review. Theor. Appl. Genet. 71: 361-374. Heinstein P.F. 1986. Plant cell suspension cultures as a source of drugs. Pharm. Int. 7:38-41. Hossain M., Karim M.R., Islam R. and Joarder O.I. 1993. Plant regeneration from nucellar tissues of Aegle marmelos through organogenesis. Plant Cell Tiss. Org. Cult. 34:199–203. Hsia C.N. and Korban S. S. 1998. Effect of growth regulators, dark treatment, and light intensity on shoot organogenesis from leaf tissue of evergreen azalea. J. Hortic. Sci. Biotechnol. 73:53-60. Hughes K.W. 1981. In vitro ecology: exogenous factors affecting growth and morphogenesis in plant tissue cultures. Environ. Exp. Bot. 21: 281–288. Ikeda T., Matsumoto T. and Noguchi M. 1977. Effects of inorganic nitrogen source and physical factors on the formation of ubiquinone by tobacco plant cells in suspension culture. Agric. Biol. Chem. 41:1197–1201. Jeong B.R., Yang C.S. and Lee E.J. 1996. Photoautotrophic growth of Dianthus caryophyllus in vitro as affected by photosynthetic photon flux and CO2 concentration. Acta Hortic. 440:611-615. Johansson L. 1983. Effects of activated charcoal in anther culture. Physiol. Plant. 59:397-403. Kassanis B. 1957. The use of tissue cultures to produce virus-free clones from infected potato varieties. Ann. Appl. Biol. 45:422-427. Knobloch K.H.and Berlin J. 1980. Influence of medium composition on the formation of secondary compounds in cell suspension cultures of Catharanthus roseus L.G. Naturforsch. 35C:551–556. Krings U. and Berger R.G. 1998. Biotechnological production of flavours and fragrances. Appl. Microbiol. Biotechnol. 49:1–8. Kumar V., Radha A. and Chitta S.K. 1998. In vitro plant regeneration of Ficus carica L. cv. Gular using apical buds from mature trees. Plant Cell Rep. 17:717-720. Lal N., Ahuja P.S., Kukreja A.K. and Pandey B. 1988. Clonal propagation of Picrorhiza kurroa Royle ex Benth. By shoot tip culture. Plant Cell Rep. 7:202–205. Lane W.D. 1979. In vitro propagation of Spirea bumalda and Prunus oistena from apices. Can. J. Plant Sci. 50:1025-1029. Lee M. and Wetzstein M. 1990. In vitro propagation of Muscadine grape by axillary shoot proliferation J. Amer. Soc. Hort. Sci. 115(2):324-329. Li J., Ou-Lee T.M., Raba R., Amundson R.G. and Last R. L. 1993. Arabidopsis mutants are hypersensitive to UV-B radiation. Plant Cell. 5:171–179. Linsmaier E.F. Skoog F. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant 15: 100-127. Lloyd G. and McCown B. 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Com. Proc. Int. Plant Prop. Soc. 30: 421-427. Mantell S.H. and Smith H. 1984. Cultural factors that influence secondary metabolite accumulation in plant cell and tissue cultures. In: Plant biotechnology., eds. Mantell S.H. and Smith H., pp. 75–108. Cambridge: Cambridge Univ. Press. Mao A.H., Wetten A., Fay M. and Caligari P.D.S. 1995. In vitro propagation of Clerodendrum colebrookianum Walp: a potential natural anti-hypertension medicinal plant. Plant Cell Rep.14:493–496. Marino C. and Bertazza G. 1990. Micropropagation of Actinidia deliciosa cvs Hayvad and Tomuri. Sci. Hortc. 45:65-74. Mathur J. and Ahuja P.S.1991. Plant regeneration from callus cultures of Valeriana wallichii. D.C. Plant Cell Rep. 9:523–526. McDonald K.A. and Jackman A.P. 1989. Bioreactor studies of growth and nutrient utilization in Alfalfa suspension cultures. Plant Cell Rep. 8:455–458. Mencuccini M. 2003. Effect of medium darkening on in vitro rooting capability and rooting seasonality of olive(Olea europaea L.) cultivars. Sci. Hort. 97:129-139. Mok M.C., Mok D.W.S, Armstrong D.J., Shudo K., Isogai Y. and Okamoto T. 1982. Cytokinin activity of N-phenyl-N’-1,2,3-thiadiazol-5ylurea(thidiazuron). Phytochemistry. 21:1509-1511. Mukherjee S.K., Sabapathi R.B. and Gupta N. 1991. Low sugar and osmotic requirements for shoot regeneration from leaf pieces of Solanum melongena L. Plant Cell Tiss. Org. Cult. 25:13–16. Murch S.J., Krishna-Raj S. and Saxena P.K. 2000. Phytomaceuticals: mass production, standardization, and conservation. Sci. Rev. Alternative Med. 4:39–43. Murashige T. 1977. Manipulation of organ culture in plant tissue cultures. Botanical Bull Acad. Sinica. 18:1–24. Murashige T. 1974. Plant propagation through tissue cultures. Ann. Rev. Plant. Physiol. 25:135-166. Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497. Nakagawa K., Konagai A., Fukui H. and Tabata H. 1984. Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep. 3:254–257. Nemth G. 1986. Induction of rooting. In Biotechnology in agriculture and forestry trees, Vol.1, ed. Bajaj Y.P.S., pp.49-64. Spring Verlag, Berlin Heidelberg. Nguyen Q.T., Kozai T., Niu G. and Nguyen U.V. 1999a. Photosynthetic characteristics of coffee (Coffea arabusta) plantlets in vitro in response to different CO2 concentration and light intensities. Plant Cell Tiss. Org. Cult. 55:133-139. Pâques M. 1991. Vitrification and micropropagation: causes, remedies and prospects. Acta. Hortic. 289: 283-290. Pasqualetto R.H., Zimmerman R.H. and Fordham I. 1988. The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tiss. Org. Cult. 14: 31–40. Patra A., Rai B., Rout G.R. and Das P. 1998. Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regulation. 24: 13–16. Payne G.F., Bringi V., Prince C. and Shuler M.L. 1991. Plant cell and tissue culture in liquid systems. Munich: Hanser Publ. pp. 1–10. Taiz L. and Zeiger E. 1998. Plant Physiology, 3rd ed. In Growth and Development. Sinauer Associates Inc., Publishers,Massachusetts. Prince R.C., Gunson D.E. 1994. Just plain vanilla? Trends Biochem. Sci. pp. 521. Principe P.P. 1989. The economic significance of plants and their constituents as drugs. In: Economic and Medicinal Plant Research, 3., eds. Wagner H., Hikino H. and Farnsworth N.R., pp. 1-17. London: Academic Press. Pryce S., Lumsden P.J., Berger F. and Leifert C. 1993. Effect of plant density and macronutrient on Delphinium shoot cultures. J. Hort. Sci. 68: 807–813. Ramachandra Rao S. and Ravishankar G.A. 2002. Plant cell cultures:Chemical factories of secondary metabolites. Biotechnology Advances. 20:101–153 Rasia S., Kantharajah A.S. and Dodd W.A. 1994. The effect of growth regulators, source of explants and irradiance on in vitro regeneration of atemoya. Aust. J. Bot. 42:333-340. Rerry P., Ueno K. and Shetty K. 1999. Reversion to hyperhydration by addition of antibiotics to remove Pseudomonas in unhyperhydrated oregano tissue cultures. Process Biochemistry. 34:717-723. Rout G.R. and Das P. 1997a. In vitro organogenesis in ginger (Zingiber officinale Rosc.). J. Herbs Spices Medicinal Plants. 4(4):41–51. Rout G.R., Mallick U.C. and Das P. 1992. In vitro plant regeneration from leaf callus of Cephaelis ipecacuanha A. Richard. Adv. Plant Sci. 5(2):608–613. Rout G.R., Samantaray S. and Das P. 2000. In vitro manipulation and propagation of medicinal plants. Biotechnol. Adv. 18:91-120. Rumary C. and Thorpe T.A. 1984. Plantlet formation in black and white spruce. I: In vitro techniques. Can. J. For. Res. 14: 10–16. Sakamoto K., Iida K., Sawamura K., Hajiro K., Asada Y., Yoshikawa T. and Furuya T. 1993. Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata. Phytochemicals. 33:357–360. Sasse F., Heckenberg U. and Berlin J. 1982. Accumulation of β-carbolin alkaloid and serotonin by cell culture of Peganum harmala L.I. Correlation between plants and cell cultures and influence of medium constituents. Plant Physiol. 69:400-404. Saxena C., Rout G.R. and Das P.1998. Micropropagation of Psoralea corylifolia Linn. J. Med. Aromatic Plant Sci. 20:15–18. Sharma N., Chandel K.P.S. and Paul. A. 1993. In vitro propagation of Gentiana kurroo: an indigenous threatened plant of medicinal importance. Plant Cell Tiss. Org. Cult. 34:307–309. Shetty K. and McKersie B.D. 1993. Proline, tioproline and potassium mediated stimulation of somatic embryogenesis in alfalfa (Medigaco sativa L.). Plant Sci. 88:185–193. Silva P. and Ricardo C.P.P. 1992. Beta-fructosidases and in vitro dedifferentiation -redifferentiation of carrot cells. Phytochemistry. 31: 1507–1511. Singh S., Ray B.K., Bhattacharyya S. and Deka P.C. 1994. In vitro propagation of Citrus reticulate Blanco and Citrus limon Burm.f. Hort. Science. 29:214-216. Skirvin R.M., Chu M.C. and Young H.J. 1990. Rose. In: Handbook of Plant Cell Cultures, Vol. 5., eds. Ammirato P.V., Evans D.R., Sharp W.R. and Bajaj Y.P.S., pp. 716–743. New York: MacMillan. Stafford A., Morris P. and Fowler M.W.1986. Plant cell biotechnology: a perspective. Enzyme Microb. Technol. 8: 19–23. Stockigt J., Obitz P., Flakenhagen H., Lutterbach R. and Endress R. 1995. Natural products and enzymes from plant cell cultures. Plant Cell Tiss. Org. Cult. 43: 914–920. Takayama S. and Misawa M. 1980. Differentiation in Lilium bulb scales grown in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiol. Plant. 48(1):121-125. Thomas T.H. and Blakesley D. 1987. Practical and potential uses of cytokinin in agriculture and horticulture. British. Plant Growth Reg. Group Monograph. 14: 69-83. Thorpe T.A. 1994. Morphogenesis and regeneration. In: Plant Cell and Tissue Culture., eds. Vasil I.K. and Thorpe T.A., pp.17–36. Kluwer Academic Publishers, Dordrecht, The Netherlands. Upadhyay R., Arumugam N. and Bhojwani S.S. 1989. In vitro propagation of Picrorhiza kurroa Royle Ex. Benth.: an endangered species of medicinal importance. Phytomorphology. 39(2,3):235–242. Van Huylenbroeck J.M. and De Riek J. 1995. Sugar and starch metabolish during ex vitro rooting and acclimatization of micropropagated Spathiphyllum “Petite”. Plant Science. 111:19-25. Vasil I.K. 1990. The realities and challenges of plant biotechnology. Bio. Technology. 6:296–301. Webster T.M. 1995. New perspectives on vanilla. Cereals Food World. 40:198–200. Webb K.J. and Street H.E. 1977. Morphogenesis in vitro of Pinus and Picea. Acta. Hort. 78: 259–269. Wiart C. 2002. Medicinal plants of Southeast Asia. 2nd ed. Prentice Hall Asia. Willibald S., Yizhong C., Thomas D., Jürgen S. and Harold C. 2001. Betalains of Celosia argentea. Phytochemistry. 58: 159–165. Wong K.Y. 1994. Chinese Herbal Medicine. Wokman Press, Hong Kong. Zenk M.H. 1991. Chasing the enzymes of secondary metabolism: plant cultures as a pot of gold. Phytochemistry. 30:3861–3863. Zhang B., Stoltz L. P. and Snyder J. C. 1987. In vitro propagation of Euphorbia fulgens. Hort. Sci. 26:1084. Ziv M. 1991. Vitrification: Morphological and physiological disorders of in vitro plants. In: Micropropagation: technology and application., eds. Debergh P.C. and Zimmerman R.H., pp. 45–69. Dordrecht, the Netherlands. Kluwer Academic Press.
摘要: 台灣有許多植物具有潛在藥用價值,近來漸受重視,本實驗利用微體繁殖方法建立五種藥用植物之增殖系統,其為青葙、台地黃及紫花地丁等三種草本植物以及食茱萸與茄苳等兩種木本植物,方法過程含括建立無菌培植體、芽體誘導與快速增殖及發根與馴化。試驗結果顯示青葙頂芽培養於MS培養基添加NAA0.03 mg/L及BA0.3 mg/L,可獲得最多芽數,而腋芽增殖之最適培養基為MS添加NAA0.01 mg/L及BA0.1 mg/L,BA濃度若達0.5 mg/L,植株外觀產生明顯變異,BA的添加亦抑制青葙植物生長,植株高度較矮,增殖之植株培養於MS中巨量元素減半加IBA0.2 mg/L之培養基,培養3週後發根率達91.07%,培養5週,植株發根率與培養於MS培養基者皆達100%。台地黃腋芽及葉皆可增殖,且葉片為較佳之培植體,培植於MS培養基中,可增殖大量芽體,添加生長調節劑會抑制腋芽增生,發根培養基MS中巨量元素減半之培養基,培養2週即可全數發根。紫花地丁增殖之培養基為MS添加NAA0.04 mg/L及BA0.4 mg/L,發根培養基為MS中巨量元素減半之培養基,培養2週可全數發根;食茱萸增殖之培養基為MS添加NAA0.01 mg/L及BA0.2 mg/L,可得到最多芽數,但培養12週後,植株開始變黑、死亡,而在WPM培養基中生長較矮壯健康,食茱萸為較不易發根的植物,發根培養基為WPM中巨量元素減半加IBA1.0 mg/L之培養基經暗處理2週再移至WPM中巨量元素減半的液體培養基加蛭石發根,培養12週後,發根率可達88.89%;茄苳增殖之培養基為WPM添加NAA0.1 mg/L及BA1.0 mg/L,發根培養基為WPM中巨量元素減半,培養2週可全數發根。
Many plants in Taiwan are can be used as medicinal herb. The aims of this study were to establish in vitro multiplication protocol of Celosia argentea L., Titanotrichum oldhami (Hemsl.) Solereder, Viola yedoensis Makino, Zanthoxylum ailanthoides Sieb. & Zucc. and Bischofia polycarpa (Levl.) Airy-Shaw. In vitro micropropagation of C. argentea L. was established to get maximum shoot multiplication rate by cultivating terminal buds on Murashige and Skoog(MS) medium supplemented with NAA0.03 mg/L and BA0.3 mg/L. Maximum shoot multiplication rate of axillary buds was achieved by cultivating on MS medium supplemented with NAA 0.01 mg/L and BA0.1 mg/L. Addition of high concentration of BA 0.5 mg/L were caused the obvious variance and inhibition of average length on explants. Rooting rate achieved 91.07% by cultivating on MS medium with half strength of macroelements and supplemented with IBA0.2 mg/L for 2 weeks. After 5 weeks, all of explants rooted on MS medium as same as cultivating on MS medium with half strength of macroelements and supplemented with IBA0.2 mg/L. In vitro micropropagation of Titanotrichum oldhami (Hemsl.) Solereder was established to get maximum shoot multiplication rate by cultivating axillary buds and leaves on MS medium without any plant growth regulator. Leaves cultivated on MS medium get more shoot multiplication rate than axillary buds. Addition of plant growth regulator were inhibited multiplication of shoot of explant. Rooting rate achieved 100% by cultivating on MS medium with half strength of macroelements in 2 weeks. In vitro micropropagation of Viola yedoensis Makino was established to get maximum shoot multiplication rate by cultivating axillary buds on MS medium supplemented with NAA 0.04 mg/L and BA0.4 mg/L. Rooting rate achieved 100% by cultivating on MS medium with half strength of macroelements in 2 weeks. In vitro micropropagation of Zanthoxylum ailanthoides Sieb. & Zucc. was established to get maximum shoot multiplication rate by cultivating axillary buds on MS medium supplemented with NAA0.01 mg/L and BA0.2 mg/L. In 12 weeks, plantlets began dying and appearing black on terminal buds. Explants grow shortly and healthily on WPM medium than MS medium. Plantlets are not easily to root on medims. Rooting rate achieved 88.89% in 12 weeks by cultivating on WPM medium with half strength of macroelements and supplemented with IBA1.0 mg/L in dark for 2 weeks, than transferred to liquid WPM medium with half strength of macroelements and vermiculites. In vitro micropropagation of Bischofia polycarpa (Levl.) Airy-Shaw was established to get maximum shoot multiplication rate by cultivating axillary buds on WPM medium supplemented with NAA0.1 mg/L and BA1.0 mg/L. Rooting rate achieved 100% in 2 weeks by cultivating on WPM medium with half strength of macroelements.
其他識別: U0005-2701200717070400
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