Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23237
標題: 台灣五種藥用植物增殖系統之建立
Establishment of multiplication protocol on five medicinal plants in Taiwan
作者: 陳信任
Chen, Shinn-Ren
關鍵字: medicinal plant
藥用植物
micropropagation
tissue culture
微體繁植
組織培養
出版社: 生命科學院碩士在職專班
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摘要: 台灣有許多植物具有潛在藥用價值,近來漸受重視,本實驗利用微體繁殖方法建立五種藥用植物之增殖系統,其為青葙、台地黃及紫花地丁等三種草本植物以及食茱萸與茄苳等兩種木本植物,方法過程含括建立無菌培植體、芽體誘導與快速增殖及發根與馴化。試驗結果顯示青葙頂芽培養於MS培養基添加NAA0.03 mg/L及BA0.3 mg/L,可獲得最多芽數,而腋芽增殖之最適培養基為MS添加NAA0.01 mg/L及BA0.1 mg/L,BA濃度若達0.5 mg/L,植株外觀產生明顯變異,BA的添加亦抑制青葙植物生長,植株高度較矮,增殖之植株培養於MS中巨量元素減半加IBA0.2 mg/L之培養基,培養3週後發根率達91.07%,培養5週,植株發根率與培養於MS培養基者皆達100%。台地黃腋芽及葉皆可增殖,且葉片為較佳之培植體,培植於MS培養基中,可增殖大量芽體,添加生長調節劑會抑制腋芽增生,發根培養基MS中巨量元素減半之培養基,培養2週即可全數發根。紫花地丁增殖之培養基為MS添加NAA0.04 mg/L及BA0.4 mg/L,發根培養基為MS中巨量元素減半之培養基,培養2週可全數發根;食茱萸增殖之培養基為MS添加NAA0.01 mg/L及BA0.2 mg/L,可得到最多芽數,但培養12週後,植株開始變黑、死亡,而在WPM培養基中生長較矮壯健康,食茱萸為較不易發根的植物,發根培養基為WPM中巨量元素減半加IBA1.0 mg/L之培養基經暗處理2週再移至WPM中巨量元素減半的液體培養基加蛭石發根,培養12週後,發根率可達88.89%;茄苳增殖之培養基為WPM添加NAA0.1 mg/L及BA1.0 mg/L,發根培養基為WPM中巨量元素減半,培養2週可全數發根。
Many plants in Taiwan are can be used as medicinal herb. The aims of this study were to establish in vitro multiplication protocol of Celosia argentea L., Titanotrichum oldhami (Hemsl.) Solereder, Viola yedoensis Makino, Zanthoxylum ailanthoides Sieb. & Zucc. and Bischofia polycarpa (Levl.) Airy-Shaw. In vitro micropropagation of C. argentea L. was established to get maximum shoot multiplication rate by cultivating terminal buds on Murashige and Skoog(MS) medium supplemented with NAA0.03 mg/L and BA0.3 mg/L. Maximum shoot multiplication rate of axillary buds was achieved by cultivating on MS medium supplemented with NAA 0.01 mg/L and BA0.1 mg/L. Addition of high concentration of BA 0.5 mg/L were caused the obvious variance and inhibition of average length on explants. Rooting rate achieved 91.07% by cultivating on MS medium with half strength of macroelements and supplemented with IBA0.2 mg/L for 2 weeks. After 5 weeks, all of explants rooted on MS medium as same as cultivating on MS medium with half strength of macroelements and supplemented with IBA0.2 mg/L. In vitro micropropagation of Titanotrichum oldhami (Hemsl.) Solereder was established to get maximum shoot multiplication rate by cultivating axillary buds and leaves on MS medium without any plant growth regulator. Leaves cultivated on MS medium get more shoot multiplication rate than axillary buds. Addition of plant growth regulator were inhibited multiplication of shoot of explant. Rooting rate achieved 100% by cultivating on MS medium with half strength of macroelements in 2 weeks. In vitro micropropagation of Viola yedoensis Makino was established to get maximum shoot multiplication rate by cultivating axillary buds on MS medium supplemented with NAA 0.04 mg/L and BA0.4 mg/L. Rooting rate achieved 100% by cultivating on MS medium with half strength of macroelements in 2 weeks. In vitro micropropagation of Zanthoxylum ailanthoides Sieb. & Zucc. was established to get maximum shoot multiplication rate by cultivating axillary buds on MS medium supplemented with NAA0.01 mg/L and BA0.2 mg/L. In 12 weeks, plantlets began dying and appearing black on terminal buds. Explants grow shortly and healthily on WPM medium than MS medium. Plantlets are not easily to root on medims. Rooting rate achieved 88.89% in 12 weeks by cultivating on WPM medium with half strength of macroelements and supplemented with IBA1.0 mg/L in dark for 2 weeks, than transferred to liquid WPM medium with half strength of macroelements and vermiculites. In vitro micropropagation of Bischofia polycarpa (Levl.) Airy-Shaw was established to get maximum shoot multiplication rate by cultivating axillary buds on WPM medium supplemented with NAA0.1 mg/L and BA1.0 mg/L. Rooting rate achieved 100% in 2 weeks by cultivating on WPM medium with half strength of macroelements.
URI: http://hdl.handle.net/11455/23237
其他識別: U0005-2701200717070400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2701200717070400
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