Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23318
標題: 台灣四種藥用植物增殖系統之建立
Establishment of multiplication protocol on four medicinal plants in Taiwan
作者: 郭明科
Kuo, Ming-Ko
關鍵字: Limnophila aromaticoides
組織培養
Laggera alata
Limnophila rugosa
Mosla scabra
鹿耳苓
擬紫蘇草
石薺寧
大葉石龍尾
出版社: 生命科學院碩士在職專班
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摘要: 摘要 台灣有許多植物具有潛在藥用價值,近來漸受重視,本實驗利用微體繁殖方法建立四種藥用植物之增殖系統,其為擬紫蘇草、鹿耳苓、大葉石龍尾及石薺寧等四種草本植物。方法過程包括建立無菌培植體、芽體誘導、快速增殖及發根。試驗結果顯示擬紫蘇草在快速增殖芽體方面,當MS培養基添加NAA 0.02 mg/L加BA 0.2 mg/L,可獲得最多芽數。在發根方面,當MS培養基中巨量元素減半並添加IBA 0.5 mg/L,培養三週後發根率達100%,其他培養基在五週後發根率皆達100%。鹿耳苓增殖之培養基為MS添加NAA 0.01 mg/L至0.08 mg/L加BA 0.1 mg/L至0.8 mg/L,芽數皆明顯多於無添加BA者,然而,在未使用生長調節劑的植株葉片,變異性最小,而添加BA的植株葉片變異性隨濃度增加而變化。發根培養基MS中巨量元素減半時,發根率皆可超過MS培養基。大葉石龍尾在快速增殖芽體方面,當MS培養基添加NAA 0.04 mg/L加BA 0.4 mg/L,可獲得最多芽數。在發根方面,當MS培養基中,巨量元素減半與否,以及添加IBA與否,培養六週後發根率皆達100%。石薺寧在快速增殖芽體方面,當MS培養基添加NAA 0.01 mg/L加BA 0.1 mg/L,可獲得最多芽數。在發根方面,在MS培養基中,巨量元素減半並添加IBA 0.2 mg/L或0.5 mg/L,發根率明顯優於無添加者。
Abstract Many plants in Taiwan could be used for medicine. The aims of this study were to establish in vitro multiplication cultural protocol of Limnophila aromaticoides, Laggera alata, Limnophila rugosa and Mosla scabra. In vitro micropropagation of Limnophila aromaticoides was established to get maximum shoots multiplication rate by cultivating terminal buds on Murashige and Skoog(MS) medium supplemented with NAA 0.02 mg/L and BA 0.2 mg/L. Rooting rate achieved 100% by cultivating on MS medium with half strength of macroelements and supplemented with IBA 0.5 mg/L for 3 weeks. After 5 weeks, all of explants rooted on MS medium as same as cultivating on MS medium with half strength of macroelements and supplemented with IBA 0.2 mg/L. In vitro micropropagation of Laggera alata was established to get more shoots multiplication rate by cultivating terminal buds on MS medium supplemented with NAA 0.01 to 0.08 mg/L and BA 0.1 to 0.8 mg/L. However, addition of plant growth regulators were caused the obvious variance on leaves. Rooting rate by cultivating on MS medium with half strength of macroelements were more than cultivating on MS medium. In vitro micropropagation of Limnophila rugosa was established to get maximum shoots multiplication rate by cultivating terminal buds on MS medium supplemented with NAA 0.04 mg/L and BA 0.4 mg/L. Rooting rate were achieved 100% by cultivating on MS medium, MS medium with half strength of macroelements, adding IBA or not into media in 6 weeks. In vitro micropropagation of Mosla scabra was established to get maximum shoots multiplication rate by cultivating terminal buds on MS medium supplemented with NAA 0.01 mg/L and BA 0.1 mg/L. Rooting rate by cultivating on the media with IBA 0.2 mg/L or 0.5 mg/L were more than cultivating on the media without IBA.
URI: http://hdl.handle.net/11455/23318
其他識別: U0005-2801200814381900
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2801200814381900
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