Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23345
標題: 茄邊鹼對膀胱癌細胞促進凋亡機制之探討
The Pro-apoptotic Activity of Solamargine on Bladder Cancer Cells
作者: 張淑玲
Chang, Shu-Ling
關鍵字: solamargine
茄邊鹼
bladder cancer
J82
膀胱癌細胞株
出版社: 生命科學院碩士在職專班
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摘要: 癌症是目前侵襲人類健康導致死亡一個主要因素,研發各種抗癌藥物是眾多學者努力研究的方向。許多的抗癌藥物,都具備誘發腫瘤細胞-自然凋亡的作用。而中草藥是發展抗癌藥物的一個重要的來源,如黃水茄萃取液對於人類肺癌、乳癌及肝癌細胞株有極佳的抑制效果。 本研究是利用J82癌細胞株為研究題材,來探討黃水茄(Solamargine),對於抑制-膀胱癌的毒殺效果與機制。由MTT實驗結果顯示,黃水茄對J82細胞株有劑量上依賴性的抑制生長情況,其半毒殺劑量為14.77ppm。膀胱癌細胞株J82以黃水茄作用後與細胞膜上TRADD、FADD結合後,誘發產生細胞內一連串Caspase 8、Caspase 3的增加;導致細胞凋亡,另外黃水茄又促使抑制細胞進行有絲分裂之細胞核內p27、p21等蛋白的表現量。由此結果推論黃水茄藉由使膀胱癌細胞進行細胞凋亡及抑制細胞分裂而達到抗癌的效果。
Solamargine is a major steroidal alkaloid glycoside that was purified from Solanum incanum plant. Solamargine displayed a superior cytotoxicity in human lung, breast and liver cancer cells. In this study, we characterized its mechanism and showed that Solamargine induces apoptosis of the bladder cancer cells. The half-inhibitory concentration (IC50) of the cell viability assay for bladder tumor cell line, J82 cells, was 14.77 ppm. Solamargine induced apoptosis was dose-dependent and increased the sub-G1 fraction when J82 cells were examined by flow cytometry. Solamargine up-regulated the expressions of external death receptors, such as TNFR-I-associated death domain (TRADD), and Fas-associated death domain (FADD). These effects resulted in the release of mitochondrial cytochrome c and activation of caspase-8 and -3 in the cells. Solamargine also increased protein expression of p27 and p21, which mediated cell cycle arrest, in the nucleus . These results indicated that Solamargine treatment inhibited cell growth by triggering extrinsic apoptotic pathway and cell cycle arrest of bladder cancer cells.
URI: http://hdl.handle.net/11455/23345
其他識別: U0005-0808200809551700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0808200809551700
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