Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23438
標題: A型流感病毒HA抗原區的生物資訊分析
Bioinformatics analysis of HA antigenic areas in influenza A viruses
作者: 魏竹君
Wei, Ju-Jiun
關鍵字: influenza A virus
A型流感病毒
Hemagglutinin
HA
Bioinformatics
血球凝集素
生物資訊
出版社: 生命科學院碩士在職專班
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摘要: A型流行性感冒是一種傳染性甚高的呼吸道感染疾病,在過去一世紀曾經發生3次的全球大流行,以1918年A型流感病毒引起的「西班牙流感」最為嚴重,估計至少有5000萬人死亡。一般認為,每年流感流行可引起約有300萬到500萬的嚴重病例,導致30到50萬人死亡。流感病毒的表面抗原非常容易產生變異,每隔一到二年就會出現新流行病毒株,能夠躲避人體的免疫系統,導致人體不具對抗新病毒的免疫力。疫苗是最有效預防流感的方法,能夠有效降低致病率與死亡率。世界衛生組織必須每年事先預測流感病毒株,才能開始製備疫苗,如預測的流感病毒株無效將會對全球人民的健康及經濟造成重大衝擊,因此通用的流感疫苗(universal vaccine)研製更顯得重要。 HA是最容易產生變異的流感病毒表面抗原之ㄧ。我們以多序列排比方式分析H1~H6共6個亞型的HA蛋白序列,試圖找出高度保留的序列片段。藉由3D晶體結構觀察這些序列的結構,以ProtScale工具分析HA蛋白序列的親水與疏水特性。再將高度保留的序列片段在UniProtKB資料庫中進行Blast,確認是否有類似的序列。 在H1~H6亞型的病毒株中都有11個胺基酸GXFGAIAGFIE((X: I or L))序列,即使有差異也僅有一個胺基酸不同,且胺基酸特性相當類似。此序列在HA蛋白序列中屬於較親水性的區域。除了流感病毒外,Blast分析結果僅Archaea有3個序列類似,Identity: 69-81,而其他物種,甚至是人類都沒有類似的序列。我們推測A型流感病毒的HA蛋白之GLFGAIAGFIE序列或許可以作為A型流感病毒通用疫苗的抗原。
Influenza A is a serious respiratory infectious disease. In the past century, three times of serious influenza pandemics occurred. “Spanish influenza” caused by influenza A virus in 1918 was the most serious one and killed at least 50 million people. Generally, influenza epidemics cause severe illness in 3000000-5000000 people and kill 30000-50000 people worldwide every year. New epidemic influenza A strains arise every 1 to 2 years by the introduction of variation within surface antigen. The new variants are able to elude human host immune system and there is therefore no lasting immunity against the virus. Flu vaccine is the most effective prevention method. It can reduce effectively the morbidity and the mortality. Every year, WHO must predict influenza virus strains beforehand, and then flu vaccine can be made in advance. If the decided influenza virus strains were not correct, there should be significant impact on the people''s health and the economy globally. To develop and make universal vaccine is then very important. One of influenza virus surface antigen that easiest occurs variants is HA. We analyzed HA protein sequences of H1~H6 six subtypes by multiple sequence alignments and attempted to find conserved segments. We observed structures of these conserved segments by 3D crystal structure, and used ProtScale tool to analysze the hydrophilic and hydrophobic scale for HA protein sequences. The conserved segments were compared using UniProtKB database via the Blast to confirm the similar sequences. The 11 amino acid sequence “GXFGAIAGFIE” (X: I or L) is present in all H1~H6 subtype virus strains. The sequence is in hydrophilic region in HA protein sequence. Besides the influenza viruses, Blast analysis result only in Archaea having 3 sequences to be similar, Identities ranged from 69% to 81%. We do not find similar sequence as to “GLFGAIAGFIE” in other species and in human. We presume that “GLFGAIAGFIE” sequence may be used as the antigen for universal influenza vaccine.
URI: http://hdl.handle.net/11455/23438
其他識別: U0005-2008200917095700
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200917095700
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