Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23584
標題: 擬南芥中與SERRATE交互作用之蛋白的功能性分析
Functional characterizations of SERRATE-interacting proteins in Arabidopsis
作者: 黃恆毅
Huang, Heng-Yi
關鍵字: 表觀遺傳學
SE
缺水反應
ATX1
GTE12
dehydration
出版社: 生物化學研究所
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摘要: 表觀遺傳的現象包括DNA甲基化、染色質重組和組蛋白修飾等。現有的證據顯示,驅動植物對環境壓力所產生的應變,和特定的組蛋白修飾有關。已知ATX1 (ARABIDOPSIS HOMOLOG of TRITHORAX 1)以及GTE12(General Transcription Factor Group E 12)能夠辨識組蛋白尾端以調控基因的表現。ATX1蛋白具有甲基轉移酶特性的SET domain,能夠進行H3K4me3。有文獻指出,調控離層酸(Abscisic Acid, ABA)生合成的NCED3可以經ATX1調控,當植物在缺水環境當中,ATX1能調節ABA的生合成量,促使氣孔關閉。實驗室利用酵母菌雙雜合篩選以及螢光融合蛋白進行細胞核中聚集觀察,得知ATX1與調控miRNA生合成的SE(SERRATE)有交互作用,因此SE可能與ATX1結合,共同調控NCED3基因的表現。GTE12具有Bromodomain,能辨識乙醯基以調控基因的表現。由於GTE12並無明顯外表型,推測GTE12參與在逆境環境的訊息調控。實驗室利用酵母菌雙雜合篩選和螢光融合蛋白進行細胞核中聚集觀察,發現SE與GTE12蛋白的C端有交互作用。透過Illumina的次世代定序技術分析gte12-3突變株,發現許多差異性的基因,其中值得注意的是CYP707A3基因,其蛋白產物為8''-hydroxylase,參與ABA的代謝。綜合以上結果,暗示SE可能藉由與ATX1和GTE12的結合,參與ABA的表觀遺傳調控。
Epigenetics is the study of changes in gene expression or cellular phenotype caused by mechanisms such as DNA methylation, chromatin remodeling and histone modifications, etc. Studies showed that specific histone modifications promote plant response to environmental stress. In Arabidopsis, ATX1 (ARABIDOPSIS HOMOLOG of TRITHORAX 1) and GTE12 (General Transcription Factor Group E 12) are able to recognize histone tail and then regulate gene expression. ATX1 has a histone methyltransferase activity, which is able to enhance gene expresion by H3K4me3 modification. It has been reported that ATX1 can regulate NCED3 to increase abscisic Acid (ABA) biosynthesis. ABA accumulation could promote stomatal closure to decrease transpiration during dehydration. SE (SERRATE) is a key regulator in miRNA processing. Using yeast two-hybrid screening and co-localization analysis, we showed interaction between ATX1 and SE. GTE12 has a bromodomain protein which is able to recognize acetylation site on histone tail and regulate genes expression. Previously, we found that the C-terminal domain of GTE12 can interact with SE by yeast two-hybrid screening. Here, RNA-seq analysis show that gte12-3 mutant can repress the gene expression of CYP707A3, which encoded a 8''-hydroxylase for ABA degradation during dehydration and rehydration. Taken togather, these results suggest that the interaction between SE and ATX1 or SE and GTE12 maybe required for epigenetic regulation of ABA response.
URI: http://hdl.handle.net/11455/23584
其他識別: U0005-2208201316042400
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2208201316042400
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