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標題: 與竹嵌紋病毒三重疊基因區第二轉譯蛋白相互作用之宿主蛋白搜尋
The Screening of Host Factor(s) Interacting with the Triple-Gene-Block Protein 2 of Bamboo Mosaic Virus
作者: 林宗旻
Lin, Tzung-Min
關鍵字: Bamboo Mosaic Virus
出版社: 生物化學研究所
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摘要: 竹嵌紋病毒 (Bamboo mosaic virus, BaMV) 是一種由感染的單子葉植物中所發現的病毒。它需要利用本身基因體三重疊基因區所轉譯出來的蛋白(Triple-Gene-Block proteins, TGBps),協助它在植物細胞中進行移動。本研究乃利用 BN-PAGE(Blue Native Polyacrylamide Gel Electrophoresis) 及 Tricine SDS-PAGE 尋找在 Nicotiana benthamiana 細胞之膜狀構造中,能夠和 BaMV 三重疊基因區第二轉譯蛋白 (TGBp2) 結合,並協助病毒蛋白核酸複合體在細胞與細胞間移動的宿主蛋白。首先,我利用差異離心方式取得健康及感染BaMV的N. benthamiana葉片中的膜狀成份 (P30),並以西方墨點分析比較健康及BaMV感染菸草植株中,TGBp2蛋白的型態,發現感病菸草中之TGBp2主要以單體、雙體及三體之型態存在。接著,我以非離子性清潔劑 (non-ionic detergent) 將可能含有 TGBp2 之蛋白複合體萃取出來,並進一步將其分成 P100 及 S100 兩部份。可能含有 TGBp2 蛋白之複合體先以 BN-PAGE 進行大小聚合體的分離,再以 Tricine SDS-PAGE 將單一複合體中的各個蛋白質分開。實驗結果顯示, P100 中含有四個蛋白複合體,其中分子量約 127 kDa 及 83 kDa 的蛋白聚合體含有 BaMV TGBp2;S100 則有五個蛋白複合體,其中分子量約 200 kDa 的蛋白複合體含有 BaMV TGBp2。利用氧化劑Cu(OP)2 處理 P100,也發現 TGBp2 單體會隨著 Cu(OP)2 的濃度增加而減少。由於 TGBp2 蛋白胺基酸序列上含有二個高度保留的 Cys,推測 TGBp2 可能藉由雙硫鍵與自身或其它蛋白形成聚合體。
Bamboo mosaic virus (BaMV) is a positive-stranded RNA virus that can infect monocotyledons. It needs Triple-Gene-Block proteins (TGBps) to move in the plant cell. Triple-Gene-Block protein 2 (TGBp2) is one of the TGBps. It helps the viral ribonucleoprotein complexes (RNP) to move between the host cells. In this study, Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) and Tricine SDS-PAGE were adopted to look for host factors in Nicotiana benthamiana, which can interact with TGBp2 and assist the movement of BaMV. To start with, differential centrifugation was used to isolate proteins in the membrane fraction (P30) of both healthy and BaMV-infected N. benthamiana. Western blot analyses of both healthy and BaMV-infected tobacco revealed that at least monomeric, dimeric and trimeric forms of TGBp2 exist in the BaMV-infected tissues.Furthermore, non-ionic detergent was used to extract membrane protein complexes possibly containing TGBp2. Following extraction, the protein complexes were separated into P100 and S100. The membrane protein complexes, possibly containing TGBp2, were examined with BN-PAGE and Tricine SDS-PAGE followed by Western blotting to identify the TGBp2-containing complexes. Four protein complexes were observed in P100 as examined by BN-PAGE. Two of them, with molecular masses of 127 and 83 kDa, respectively, were TGBp2-associated. Five protein complexes were detected in S100 and only the one with a molecular mass of 200-kDa had TGBp2. The decrease in the content of monomeric TGBp2 in P100 in response to the treatment of the oxidizing agent, Cu(OP)2, suggested that TGBp2 may use disulfide bridge to bond with itself or other host factors。
其他識別: U0005-1907200616032000
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