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標題: Stenotrophomonas maltophilia 之 RpfF 的晶體結構與 RpfB 的結晶研究:透過集約淬息反應可發展新型抗生素的潛力目標蛋白
Crystal Structure of RpfF and crystallization of RpfB from Stenotrophomonous maltophilia : Potential Target Proteins for Novel Antibiotics Development via Quorum Quenching
作者: 林仙雅
Lin, Hsien-Ya
關鍵字: Stenotrophomonas maltophilia
Quorum Quenching
Stenotrophomonas maltophilia
出版社: 生物化學研究所
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摘要: Stenotrophomonas maltophilia藉由釋放訊息分子 DSF ( diffusible signal factor, cis-11-methyl-2-dodecenoic acid ) 以調控其主要的致病機制。該訊息分子的合成係賴rpf ( regulation of pathogenicity factors ) gene cluster 中所編譯的蛋白 RpfF 及RpfB 來完成。有鑒於 S. maltophilia 具高度多重抗藥性,對於免疫抑制或身體抵抗力差的病人常造成致命的嚴重後果,因此若能抑制 DSF 合成,或阻斷其訊息傳遞路徑,應可為對抗細菌感染提供一新的醫療途徑。我的研究工作係針對此二重要酵素蛋白做其結構與功能分析。 利用生物資訊分析推測 RpfB為 long chain fatty acid CoA ligase ,而 RpfF 則屬於 enoyl CoA hydrotase 。目前 RpfB 已利用 Escherichia coli 為表現載體,可大量獲得蛋白並純化,現正多方嘗試其結晶條件。另外,RpfF 則已獲得解析度為 2.25 Å 的 RpfF蛋白結構,可看到晶體中 RpfF 以三聚體的形式組成一個不對稱單元,此為典型 enoyl CoA hydratase 的多聚體型態。然與其他已知結構者相比,其 CoA 結合區域卻被一
Stenotrophomonas maltophilia has emerged as a critical noncosomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combating this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia, with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF (SmRpfF) function via small molecule interference may constitute a new way of treating S. maltophilia infection. SmRpfF has been overexpressed in Escherichia coli, purified and crystallized using hanging-drop vapour-diffusion method. The crystals belong to the tetragonal space group P41212 containing three protein molecules per asymmetric unit, with unit-cell parameters a = b = 148.51, c = 122.82 Å, and were diffracted to a resolution of 2.25 Å. Our results indicate that SmRpfF belongs to the enoyl-CoA hydratase / enoyl-CoA isomerase family, but with some distinct features; the acyl-CoA substrate entrance is blocked to a great extent by re-orientated C-terminal
其他識別: U0005-2107201014181000
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