Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/24259
標題: Cav3.2 T型鈣離子通道基因剔除鼠右腦基因晶片資料集之生物資訊研究
A Bioinformatic Study on The Right-Hippocampal Microarray Datasets of Cav3.2 T-type Calcium Channel Knockout Mouse Strain
作者: 王莉雅
Wang, Li-Ya
關鍵字: 微陣列
microarray
鈣離子通道
學習
記憶
calcium channels
learning
memory
出版社: 資訊管理學系所
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摘要: 本研究以微陣列基因晶片資料,獲得Cav3.2 T型鈣離子通道剔除小鼠在接受痕基恐懼實驗前後右側海馬內的基因表現數據,以統計學方法確認小鼠在訓練前後右側海馬內基因表現量是否具有顯著差異。針對右腦的基因表現量來做探討,了解右腦基因表現是否與T型鈣離子通道的缺失相關,篩選出野生型與基因剔除小鼠的差異表達基因,希望在大量的數據中可找到與學習、記憶相關的標的。在此論文先將資料篩選後使用成對樣本T檢定(Paired-Samples T-Test)的結果,再利用迴歸分析(Regression Analysis)將表現具有顯著差異的基因列出。研究結果顯示,其基因為Igf1r、Abcd2、Wdr33、Ptprs、Pds5b、Rfxap…六項,並應用NCBI、nextprot、GO(Gene Ontology groups)等資料庫進行比對,未來這些篩選出的基因功能希望能被腦生物科學所應用與驗證。
The purpose of this study was to compare differential gene expression for the right hemisphere of Cav3.2 gene knockout mouse and the wild type based on genome wide microarray datasets after contextual trace fear conditioning. First, Paired-samples t-test was conducted to confirm whether there were significant differences of gene expression prior to and after experimental training. Secondly, linear regression analysis was applied to select target genes which have statistical significant differences in gene expression. After that, the target genes were then put into the web-based biological databases for validation and comparison of their association with the learning and memory for brain. The results showed that there are six genes which were selected by this method, Igf1r、Abcd2、Wdr33、Ptprs、Pds5b、Rfxap are somewhat related to brain learning, memory and other functions as indicted by some biological references. In the future, these selected genes could be used for further functional investigation in brain science study.
URI: http://hdl.handle.net/11455/24259
其他識別: U0005-2707201201231300
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2707201201231300
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