Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/25082
標題: 探討飼料原料之醱酵速率及其對白肉雞之影響
Study on fermentation rate of feedstuffs and its effects in broiler
作者: 鄭筑梵
Cheng, Chu-Fan
關鍵字: Chicken

Automated pressure evaluation system
Fermentation rate
體外氣體生成系統
醱酵速率
出版社: 動物科學系所
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摘要: 本研究以不同飼料原料為基質,新鮮之雞隻排泄物作為接種物,使用in vitro體外氣體生成系統,進行體外醱酵模擬試驗,比較不同飼料原料醱酵後產生之氣體生成量、動力學參數、終產物(揮發性脂肪酸及氨)之生成量及有機質消失率,進一步評估不同醱酵速率之穀物飼糧對白肉雞之影響。試驗以18種飼料原料,包括穀物(玉米、脫殼大麥、米、小麥及樹薯)、加工副產物(小麥麩皮及粉頭)、加工原料(乾爆玉米、擠壓玉米及烘烤馬鈴薯)、蛋白質來源 (大豆粕、菜籽粕、醱酵豆粕、玉米筋粉、魚粉及肉骨粉)及可溶物乾燥酒糟(玉米及高粱)進行體外氣體生成試驗。結果顯示,加工原料及米之醱酵速率較穀物及加工副產物為快,而蛋白質來源及可溶物乾燥酒糟最慢;供試各原料之總氣體生成量、乾物質消失率與其揮發性脂肪酸產生量三者呈一致之趨勢,氨濃度則與揮發性脂肪酸及氣體生成量呈現相反之趨勢,蛋白質原料顯著比其他原料產生較高量之氨。原料體外消化後之殘渣,其氣體生成量較原料為低,幾乎所有原料之氣體生成量皆降低,只有樹薯(Cassava)呈現與消化水解前相同之產氣量,加工原料經體外消化水解後之產氣量比穀物原料低,與未經消化水解之原料的結果相反,而蛋白質原料經消化水解後之氣體生成量較原料更低。根據體外氣體生成試驗之結果,選擇兩種不同醱酵速率之穀物原料(脫殼大麥及小麥),配製等蛋白、等能量之試驗飼糧,並以玉米飼糧為對照飼糧,進行雞隻飼養試驗,試驗結果顯示,醱酵速率快之脫殼大麥飼糧組之飼料轉換率與玉米飼糧無差異,但顯著優於醱酵速率慢之小麥飼糧組 (p<0.05);微生物組成方面,小麥組在21日齡降低盲腸厭氧菌及結直腸好氧菌之菌數(p<0.05) ;小麥飼糧組之盲腸pH值及氨含量,顯著低於脫殼大麥飼糧組與玉米飼糧組 (p<0.05)。綜上所述,以體外氣體生成系統配合動力學參數及終產物之分析,可評估不同飼料原料於雞隻之體外氣體生成模式,而脫殼大麥及小麥於in vitro醱酵過程雖有差異,且確實改變雞隻腸道環境,但雞隻本身生長會受到諸多因素之影響,因此無法確定原料之醱酵速率是否為造成生長試驗差異之主要因素,故仍需更深入之試驗以進行探討。
The purpose of this study was to investigate the in vitro fermentation patterns of different feedstuffs by automated pressure evaluation system (APES) using chicken fecal inocula, kinetic parameters, end-productions and dry matter loss(%) to evaluate the fermentation characteristics of feed ingredients effects in broiler. Eighteen feedstuffs included cereals (corn, barley, rice, wheat, and cassava), cereal byproducts (wheat bran, and wheat middling), processed feedstuffs (popping corn, extrusion corn, and roasted potato), protein sources (SBM, rapeseed meal, fermented soybean meal, corn gluten meal, fish meal, and meat bone meal,) and distiller's dried grains soluble (DDGS) (corn and milo) were used to evaluate the fermentation characteristics. The results indicated the processed feedstuffs and rice were fermented more rapidly than cereals and cereal byproducts , the protein sources and DDGS are the least. The total gas volume, dry matter losses and SCFA showed similar trend in test samples. But the ammonia concentration trend was opposite. After in vitro digestion, the gas productions of all feedstuffs decreased except for cassava. The gas production of processed feedstuffs was lower than cereals and protein sources lower than without in vitro digestion. According to the in vitro results, we chose two feedstuffs with different fermentation rate, dehulled barley (fast) and wheat (slowly), and the corn diet as the control for in vivo feeding experiment. The data suggested the feed conversion ratio (feed / gain, FCR) of dehulled barley and corn diet were better than wheat diet (1.73, 1.70 verse 1.81). The wheat diet decreased the anaerobic bacteria count in caecal and aerobic bacteria count in colon at 21day. In ammonia concentration and pH value, the wheat diet was lowest. In conclusion, the APES is to go with kinetic parameters and end-productions analysis could predict in vitro fermentation patterns of different feedstuffs. Although in vitro gas fermentation were different between dehulled barley and wheat and the intestine condition accurately changed, the growth of broiler is involved in many factors. We are not sure that fermentation rate is a major factor in broiler growth. More experiments are necessary for confirmation of this hypothesis.
URI: http://hdl.handle.net/11455/25082
其他識別: U0005-2508200813552900
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