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標題: 台灣土雞肝臟差異表現基因GST-CL3、Otrf、FASN、INSIG1、apoVLDLⅡ及apo B基因單一核苷酸多態性之研究
Single Nucleotide Polymorphisms of GST CL3, Otrf, FASN, INSIG1, apoVLDL II , and apoB Genes Differentially Expressed in Liver of Taiwan Country Chickens
作者: 陳子翰
Chen, Tzu-Han
關鍵字: Liver
Taiwan country chickens
出版社: 動物科學系所
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摘要: 本試驗之目的為利用土雞肝臟組織差異表現基因來探討差異表現基因之基因多態性。以PCR單股構形多態性分析法(single-strand conformation polymorphisms, PCR-SSCP)找出差異表現基因之基因態樣。麩胱苷肽轉移酶CL3次單元(glutathion S-transferase CL3 subunit, GST CL3)、卵運鐵蛋白酶(ovotransferrin, Otrf)、脂肪酸合成酶(fatty acid synthase, FASN)、胰島素誘導基因1(insulin induced gene 1, INSIG1)、脫輔基蛋白Ⅱ(apolipoprotein VLDLⅡ, apoVLDLⅡ)及脫輔基蛋白B(apoprotein B, apoB)等基因於聚丙烯醯胺膠片中皆呈現基因多態性。利用序列分析法偵測差異表現基因之SNP點。以PCR-RFLP及微序列分析法(minisequencing method)進行SNP之確認,所得結果一致。利用微序列法分析6個差異表現基因之SNP基因型並計算基因型頻率。發現Otrf基因在L2品系(G70C)、(T106C)、(C161T)及(A269G)變異皆為GG/TT/CC/GG之純合型。FASN基因(C41T)及apoVLDLⅡ基因(C93T)SNP點在L2與B品系皆以CC型最多。apoB1基因(T42C)在L2品系II型較ID及DD型為多,顯示這些結果為產蛋優勢之基因型。進一步以基因型與性狀資料進行統計分析之結果顯示,L2品系土雞之GST CL3 基因(T221C)之TT型雞之總產蛋數顯著高於TC及CC兩型者(P < 0.05)。apoVLDLⅡ基因(C93T),其CC型雞在2及4周齡體重高於CT型者(P < 0.01)。apoB1基因II型對之平均初產日高於ID及DD型者(P < 0.05)。以上試驗結果可作為土雞育種上候選基因之參考。
The aim of this study was to inverstigate the single nucleotide polymorphisms of the different expression genes in Taiwan country chicken livers. PCR-SSCP was used to find the gene polymorphisms and mutations. Glutathione S-transferase CL3 subunit (GST-CL3), ovotransferrin (Otrf), fatty acid synthase (FASN), insulin induced gene 1 (INSIG1), apolipoprotein VLDLⅡ (apoVLDLⅡ), and apoprotein B (apoB) genes were all found polymorphisms by PCR-SSCP. Sequencing was performed to find the SNPs. The genotypes obtained from PCR-RFLP were the same as minisequencing. The SNPs genotyping and frequency analysis of six differentially expressed genes in livers were carried out. The genotypes of Otrf (G70C), (T106C), (C161T) and (A269G) gene in L2 line were all GG/TT/CC/GG homozygotes. Most CC genotype of FASN (C41T) and apoVLDLⅡ (C93T) can be detected in both L2 and B lines. The frequency of genotype II of apoB1 (T42C) gene was more than the genotype ID and DD in the L2 line. It revealed that the genotype II of apoB1 (T42C) was more advantage for the reproductive traits especially significant on the 1st egg day. The relationship between genotypes and traits were investigated. The results showed that the total number eggs of TT type of GST CL3 (T221C) gene were higher than the TC and CC types in L2 line significantly (p < 0.05). The body weight at 2 and 4 wks of CC type of apoVLDLⅡ (C93T) gene was heavier than the type CT (p < 0.01). The age at 1st egg of apoB1 type II was significant early than the type ID and DD (p < 0.05). These results could provide the information for Taiwan country chicken selection in Taiwan.
其他識別: U0005-3107200814245800
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