Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/25324
標題: 產蛋褐色菜鴨受飼糧T-2 toxin毒性效應之影響
Influence of Dietary T-2 Toxin Toxicity on Laying Brown Tsaiya
作者: 左克華
Tso, Ko-Hua
關鍵字: T-2 toxin
T-2 toxin
antioxidant
Brown Tsaiya
抗氧化劑
褐色菜鴨
出版社: 動物科學系所
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摘要: 本研究由三方面探討產蛋褐色菜鴨受飼糧中T-2 toxin (T-2毒素,T-2)毒性效應之影響。試驗一為建立高效液相層析儀檢測飼料中T-2和HT-2 toxins (HT-2) 含量之分析方法。結果為使用超純水/甲醇 (10:90, v/v)加氯化鈉之萃取液,添加1-氰酸悤 (1-AN)和4-二甲基胺基吡啶 (4-DMAP)於50℃進行衍生化反應,搭配參照Visconti et al. (2003)法修訂之梯度沖提,定量T-2和HT-2可得較高之回收率及解析度,且縮短每一樣品分析時間2分鐘。試驗二為獲致T-2,將商業蛋鴨料於15℃,相對溼度86%下進行培養21天經萃取得到T-2。試驗三使用試驗二之純化T-2進行動物試驗,試驗期間共6週,第一階段為毒素添加期,3種不同之飼糧添加量(0、0.5及5 mg T-2/kg)為試驗之主效應,將之逢機分配於每列6籠且每籠1鴨隻之6列產蛋鴨,無及有添加綜合抗氧化劑為副效應,逢機分配至以列為區集之6籠鴨隻,為期4週;第二階段為恢復期,停止添加T-2於飼糧但綜合抗氧化劑持續添加,為期2週。結果顯示,T-2添加量相同下餵飼添加綜合抗氧化劑飼糧之產蛋鴨,其體重和每日體增重顯著高於餵飼未添加綜合抗氧化劑者 (P < 0.05)。羽被評分方面,餵飼添加T-2飼糧之產蛋鴨於第4週出現脫羽,且羽被評分受飼糧之T-2添加量影響而劣化 (P < 0.001),餵飼添加綜合抗氧化劑飼糧者可減緩產蛋鴨之脫羽程度。第2週起餵飼添加5 mg/kg T-2飼糧之產蛋鴨,其產蛋率顯著低於餵飼未添加T-2飼糧者 (P < 0.05)。蛋重、蛋白高度和豪氏單位分別於2、5和6週受飼糧之T-2添加量影響而增加 (P < 0.05)。產蛋鴨血漿白蛋白和鹼性磷酸酶之含量於第5週受飼糧之T-2添加量影響而減少 (P < 0.05),顯示餵飼添加T-2飼糧之產蛋鴨其腎臟功能受損,而飼糧添加綜合抗氧化劑者減緩該損害。產蛋鴨體內T-2和HT-2蓄積率隨餵飼之飼糧T-2添加量增加而增加 (P < 0.05),添加綜合抗氧化劑對其無影響。產蛋鴨餵飼添加T-2之飼糧,其血液所測得之毒素以T-2為主,其排泄物及蛋則以HT-2為主,又2週之恢復期無法使其體內T-2和 HT-2代謝殆盡。綜上所述,餵飼添加T-2飼糧之菜鴨會造成體重減少、脫羽、產蛋率下降、蛋品質改變及腎功能受損等影響,而飼糧添加綜合抗氧化劑者可部分抵消這些症狀。綜合抗氧化劑可中和T-2和HT-2所產生之自由基以抵銷其毒性,卻對T-2和HT-2於鴨隻體內之代謝並無影響。欲使所產鴨蛋之T-2和HT-2殘留量低至無法驗出,產蛋鴨停止餵飼添加T-2飼糧之恢復期須長於2週。
This study included three experiments to investigate influence of dietary T-2 toxicity on laying Brown Tsaiya (LBT). In experiment 1, a method using high performance liquid chromatography (HPLC) to determine contents of T-2 and HT-2 in feed was developed. The method with the best recovery and resolution might be obtained following procedures in order as extraction with 90% methanol plus NaCl, derivatization reaction with 1-AN and 4-DMAP at 50℃ and determination by HPLC with the modified gradient originated by Visconti et al. (2003). In experiment 2, T-2 was obtained by culturing commercial feeds for laying duck at 15℃ and relative humidity 86% for 21 days followed with extraction and purification. Experiment 3 consisted of toxin administration stage lasting for the first 4 wk and recovery stage lasting for the last 2 wk in a 6-wk experimental period. During toxin administration stage, the dietary supplementation levels (0, 0.5 and 5 mg/kg) of T-2 obtained in experiment 2 regarded as main effects were randomly allotted to 6 rows. Each row including 6 individual cages, each with one LBT, was futher split into two halves, one fed with diet supplemented with complex antioxidants and the other fed without, which were regarded as sub effects. The results showed that body weight and average daily gain of the ducks fed diets supplemented with complex antioxidants were greater than those of the ducks fed diets without under the same T-2 supplementation level (P < 0.05). Ducks fed diets supplemented with T-2 appeared of feather lost in 4th wk, and their feathering score got worse as dietary supplementation of T-2 increased (P < 0.001). Ducks fed the diets supplemented with complex antioxidants were improveed in feathering. Ducks fed diets supplemented with 5 mg T-2/kg were lower in egg production than were those fed diets not supplemented with T-2 (P < 0.05). Weight, white height and Huagh unit of the eggs produced were increased by feeding the ducks with diets supplemented of T-2 measured at 2, 5, and 6 wk, respectively (P < 0.05). Albumin and alkaline phosphatase in blood plasma measured at 5 wk were reduced by feeding the ducks with diets supplemented of T-2 (P < 0.05) implying that kindney function was damaged by dietary supplementation of T-2 and supplementation of complex antioxidants could correct the damage. Retention of T-2 and HT-2 in the laying ducks increased as dietary supplementation of T-2 increased (P < 0.05) whereas no influence on retention of the toxin was observed with dietary supplementation of complex antioxidants. T-2 was the main toxin appeared in blood, whereas HT-2 was in egg and excreta. Ducks could not metabolize T-2 and HT-2 entirely within 2-wk recovery period. In conclusion, ducks are decreased of body weight and egg production, lost of feathers and changed of egg equality by dietary supplementation of T-2. Supplementation of complex antioxidants may reduce, at least partially, the adverse effects of T-2 toxin. Complex antioxidants do counteract adverse effects by free redicals which are believed of the toxicities generated by T-2 and HT-2 and do not influence the metabolism of T-2 and HT-2 in ducks. T-2 is mainly metalobized to HT-2 in duck. Should T-2 and HT-2 contents in eggs be as low as undetectable, laying ducks have to be ceased of feeding T-2 toxin containing diets for more than 2 weeks.
URI: http://hdl.handle.net/11455/25324
其他識別: U0005-2108201019382300
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2108201019382300
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